Quantitative Bio-Science

Institute of Natural Science, College of Natural Sciences, Keimyung University (계명대학교 자연과학연구소)
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Stachys riederi var. japonica Extract Reduces Cytochrome C Release from Mitochondria in UVA-irradiated Human Dermal Fibroblasts

  • Hwang, Ji Yeon (Department of Research and Development, Research Center for Natural Ingredients & New Materials, Daepyung Co., Ltd.) ;
  • Lee, Jae Soon (Department of Cosmetic & Beauty Design, Gyongbuk Science College) ;
  • Kim, Young Chul (Major in Public Health, College of Natural Science, Keimyung University)
  • Received : 2018.10.21
  • Accepted : 2018.11.16
  • Published : 2018.11.30
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Abstract

This study was performed to investigate the cytoprotective effects of Stachys riederi var. japonica ethanol extract (SREE) to control oxidative stress induced by UVA-irradiation by examining antioxidant capacity and gene expression of cytochrome c using human dermal fibroblasts. The total polyphenolics and flavonoids in the SREE were 41.2 and 25.4 mg/g, respectively. At concentrations of 500 and $1000{\mu}g/mL$, the electron-donating ability of SREE was 48.6% and 82.0%, respectively, and the 2,2'-azino-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity was 62.3% and 78.8%, respectively. These findings showed that SREE has a fairly good antioxidant capacity. As determined by an MTT assay, the maximum permissible level for treating SREE to human dermal fibroblasts was shown to be over $200{\mu}g/mL$. SREE ($200{\mu}g/mL$) significantly decreased cytochrome c mRNA and protein expression by 31.1% (p<0.001) and 38.8% (p<0.01), respectively. These findings suggest that SREE may protect human skin cells against mitochondrial-dependent apoptosis. Therefore, SREE seems to be a natural antioxidant to protect cells against oxidative stress induced by UVA-irradiation.

Keywords

References

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