한국자원식물학회지 (Korean Journal of Plant Resources)

  • 제31권4호
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  • Pages.355-362
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  • 2018
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  • 1226-3591(pISSN)
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  • 2287-8203(eISSN)
한국자원식물학회 (The Plant Resources Society of Korea)
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코끼리마늘(Allium ampeloprasum L.)의 기내 미숙총포 배양을 통한 다신초유도와 종구대량증식 시스템

Multiple Shoot Induction and Bulb Mass Proliferation System by in Vitro Immature Spathe Culture of Elephant Garlic (Allium ampeloprasum L.)

  • Kwon, Young Hee (Chungcheongbuk-do Agricultural Research & Extension Services) ;
  • Jeong, Jae Hyun (Chungcheongbuk-do Agricultural Research & Extension Services) ;
  • Lee, Jae Sun (Chungcheongbuk-do Agricultural Research & Extension Services) ;
  • Jeon, Jong Ok (Chungcheongbuk-do Agricultural Research & Extension Services) ;
  • Park, Young Uk (Chungcheongbuk-do Agricultural Research & Extension Services) ;
  • Min, Ji Hyun (Chungcheongbuk-do Agricultural Research & Extension Services) ;
  • Chang, Who Bong (Chungcheongbuk-do Agricultural Research & Extension Services) ;
  • Lee, Sang Young (Chungcheongbuk-do Agricultural Research & Extension Services) ;
  • Youn, Cheol Ku (Chungcheongbuk-do Agricultural Research & Extension Services) ;
  • Kim, Ki Hyun (Chungcheongbuk-do Agricultural Research & Extension Services)
  • 투고 : 2017.11.09
  • 심사 : 2018.03.13
  • 발행 : 2018.08.31
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초록

본 연구는 증식이 어려운 코끼리마늘(Allium ampeloprasum L.)의 종구 보급을 위해 기내배양 기술을 이용한 대량증식 시스템을 개발하기 위해 수행되었다. 코끼리마늘의 미숙총포를 수확 직후 길이별로 채취하여 MS배지에 NAA와 kinetin 생장조절제를 혼합 농도 처리하여 배양한 결과, 10 cm 길이로 채취하였을 때가 절편체당 신초수 14.9개로 가장 많이 얻어졌다. 생장조절제 농도에 따른 생장을 조사한 결과, kinetin 2 mg/L와 NAA 0.5 mg/L를 첨가한 MS배지에서 절편체당 신초수가 14.9개로 가장 많았고 초장은 11.3 cm였으며, 생체중은 2.5 g으로 가장 좋았다. 코끼리마늘 기내소구 형성에 적합한 최적 배지조성은 생장조절제 kinetin 2 mg/L, NAA 0.5 mg/L를 첨가한 MS 배지에 다신초 유기물질인 adenine 30 mg/L를 첨가하였을 때 구 형성률이 48%로 높았으며, 이 배지에 sucrose 7%를 첨가하였을 때 기내종구 형성률이 98.2%로 증가하였다. 다신초 유도를 위한 계대배양은 1차 배양 단계보다 2차 계대배양 단계에서 신초 증식배율이 좋았으며, 2차 계대배양에서 구 형성률은 80%로 높게 나타났다.

This study was performed to develop the mass propagation system using tissue culture technique to supply the seeds of Elephant garlic (Allium ampeloprasum L.) which has difficulty in propagation. Immature spathe of Elephant garlic was cultured on Murashige & Skoog (MS) medium supplemented with two plant growth regulators, naphthaleneacetic acid (NAA) and kinetin. After 6 weeks of culture, the highest number of shoot (14.9/explant) was obtained when the immature spathe with 10 cm length was cultured right after harvesting. In MS medium supplemented with 2 mg/L kinetin and 0.5 mg/L NAA, the most vigorous growth characteristics was observed, the shoot number was 14.9/explant, its length was 11.3 cm, and its fresh weight was 2.5 g. When the bulblets were cultured in MS medium with 2 mg/L kinetin and 0.5 mg/L NAA, the addition of 30 mg/L adenine improved their proliferation and growth significantly, the highest bulblet formation rate (48%) was obtained. The addition of 7% sucrose also increased the bulblet formation rate at the highest frequency of 98.2%. The shoots were shown be more vigorously proliferated at the secondary subculture stage rather than primary culture stage, their propagation rate was 80% after subculture.

키워드

참고문헌

  1. Ariga, T., H. Kumagai, M. Yoshikawa, H. Kawakami, T. Seki, H. Sakurai, I. Hasegawa, T. Etoh, H. Sumoyoshi, T. Tsuneyoshi, S. Sumi and K. Iwai. 2002. Garlic-like but odorless plant Allium ampeloprum 'Mushuu- ninniku'. J. Japan. Soc. Hort. Sci. 71:362-369.
  2. Choi, S.Y., K.Y. Paek and J.T. Jo. 1992. Micropropagation through apical meristem, flower stalk and flower bud cultures in Allium sativum L. Korean J. Plant Tiss. Cult. 19:337-342.
  3. Han, B.H., B.W. Yae, D.H. Goo and J.Y. Ko. 1999. Effects of inorganic salts in MS medium, sucrose and activated charcoal on bulblet formation from in vitro bulb scales in Lilium Oriental hybrid 'Casa Blanca'. J. Kor. Soc. Hort. Sci. 26: 103-107.
  4. Han, S.G., C.H. Kang, I.Y. Choi, S.R. Choi, J.J. Lee, H.C. Lim, N.K. Oh and J.S. Choi, 2008. Bulb propagation on Nerine by tissue culture. Korean J. Plant Res. 21(2):134-138.
  5. Haruki, K., K. Yamada, T. Hosoki and K. Ohta. 1996. Effects of sugar and temperature on the growth of miniature bulblets of Lilium japonicum Thunb cultured on a rotary shaker. J. Jap. Soc. Hort Sci. 65:363-371.
  6. Hwang, H.Y. and Y.B. Lee, 2008. Introduction of two-step culture method for multiple seed bulb development from shoot tip culture of garlic (Allium sativium). J. Plant Biotechnol. 35:75-80
  7. Jeong, E.A., H.M. Choi, K.B. Jeon, S.A. Moon and S.T. Kwon. 2017. Enhance efficiency production of virus free commercial ginger plants. Proceedings of Symposium. September 2017. Korean J. Plant Res. p. 300.
  8. Kageyama, C., T. Komatsuda and K. Nakajima. 1990. Effects of sucrose concentration on morphology of somatic embryos from immature soybean cotyledons. Korean J. Plant Tiss. Cult. Lett. 7(2):108-110.
  9. Kaska, A., F.C. Toprak and A.R. Alan. 2013. Gynogenesis induction in leek (Allium ampeloprasum L.) breeding materials. Curr. Opinion Biotechin. 24S:28-47.
  10. Kastner, U., A. Klahr, E.R.J. Keller and R. Kahane. 2001. Formation of onion bulblets in vitro and viability during medium-term storage. Plant Cell Rep. 20:137-142.
  11. Kim, K.S., Y.S. Song, Y.S. Jang, S.S. Nam, I.H. Choi and J.K. Bang. 2006. Production of virus-free bulblets of garlic (Allium sativum L.) by meristem-tip culture of immature vegetative spathes. Korean J. Hort. Sci. Technol. 24:441-446.
  12. Kovac, M. and M. Ravnikar. 1998. Sucrose and jasmonic acid interact in photosynthetic pigment metabolism and development of potato (Solanum tuberosum L. cv. Sante) grown in vitro. Plant Growth Reg. 24:101-107.
  13. Koo, W.L., J.H. Cho, C.C. Park, Y.S. Ahn and C.B. Park. 2011. Effect of plant growth regulators on in vitro cultured atractylodes hybrid 'Dachul' (A. macrocephala x A. japonica). Korean J. Plant Res. 24(5):591-598. https://doi.org/10.7732/kjpr.2011.24.5.591
  14. Kwon, Y.S.,C.W. Kim, J.H. Kwak, M.S. Choi, J.W. Lee and I.H. Choi. 2016. Virus infection and propagation efficiency from immature spathe culture in garlic (Allium sativum L.). Korean J. Hort. Sci. Technol. 34(Suppl II): 131-132.
  15. Lee, E.M. and Y.B. Lee. 1994a. Systemic propagation of high quality garilc (Allim sativum L.) through shoot apicial meristem culture. I. Organogenesis from in vitro cultured shoot-tips. Korean J. Plant Tiss. Cult. 21:161-166.
  16. Lee, E.M. and Y.B. Lee. 1994b. Systemic propagation of high quality garlic (Allim sativum L.) through shoot apical meristem culture. II. Effects of sucrose concentration and nitrogen source on in vitro formation of bulblets. Korean J. Plant Tiss. Cult. 21:193-199.
  17. Nagakubo, T., A. Nagasawa and H. Ahkawa. 1993. Micro propagation of garlic (Allim sativum L.) through in vitro bulblet formation. Plant Cell Tiss. Organ Cult. 32:175-183.
  18. McCollum, G.D. 1987. Onion and allies: In Simmonds, N.W. (ed.), Evolution of Crop Plants. Longman S.&T., England. pp. 186-190.
  19. Nam, S.I., J.H. Park, J.I. Choi, K.S. Kwon and J.S. Uhm. 2002. Commercial production of seed garlic (Allim sativum L.) by tissue culture technique. Korean J. Plant Biotech. 29:171-177.
  20. Paek, K.Y., M.O. Oh and J.K. Choi., 1985. In vitro masspropagation of cordyline and scindapsus. Korean J. Horti. Sci. 26:83-92.
  21. Song, W.S. 2004. Direct plant regeneration and bublet formation from apical meristems culture in Allium wakegi Araki. Korean J. Plant Res. 17(1):1-6.
  22. Suh, S.K. and H.G. Park. 1988. Somatic embryogenesis and plant regeneration from flower organ culture of garlic (Allium sativum L.). Korean J. Plant Tiss. Cult. 15:121-132.
  23. Suh, S.K. and H.G. Park. 1993. Rapid multilpication through immature bublil cultures of garlic (Allim sativum L.). Korean J. Soc. Hort. Sci. 34:173-178.
  24. Yang, S.G., H.S. Lee, W.J. Jeong, S.R. Min and J.R. Liu. 1993. Production of virus-free microbulbs of garlic (Allium sativum L.) by in vitro culture of vegetable and floral buds in immature involucre. Korean J. Soc. Hort. Sci. 34:179-183.