Annals of dermatology

Korean Dermatological Association (대한피부과학회)
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The Effect of Adiponectin on the Regulation of Filaggrin Expression in Normal Human Epidermal Keratinocytes

  • Choi, Sun Young (Department of Dermatology, Inje University Seoul Paik Hospital, Inje University College of Medicine) ;
  • Kim, Min Jeong (Department of Dermatology, Chung-Ang University Hospital, Chung-Ang University College of Medicine) ;
  • Ahn, Ga Ram (Department of Dermatology, Chung-Ang University Hospital, Chung-Ang University College of Medicine) ;
  • Park, Kui Young (Department of Dermatology, Chung-Ang University Hospital, Chung-Ang University College of Medicine) ;
  • Lee, Mi-Kyung (Department of Laboratory Medicine, Chung-Ang University Hospital, Chung-Ang University College of Medicine) ;
  • Seo, Seong Jun (Department of Dermatology, Chung-Ang University Hospital, Chung-Ang University College of Medicine)
  • Received : 2018.05.08
  • Accepted : 2018.06.15
  • Published : 2018.12.01
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Abstract

Background: Adiponectin, an adipokine secreted from adipocytes, affects energy metabolism and also shows anti-diabetic and anti-inflammatory properties. Recent studies have reported that adiponectin plays a role in regulating skin inflammation. Objective: This study aimed to investigate the effect of adiponectin on the expression of filaggrin (FLG) in normal human epidermal keratinocytes (NHEKs). Methods: NHEKs were serum-starved for 6h before being treated with adiponectin. Afterward, cell viability was assessed by MTT assay. We also treated with calcium, interleukin (IL)-4, and IL-13 to provide positive and negative comparative controls, respectively. Gene mRNA expression was quantified using real time reverse transcription polymerase chain reaction, and protein expression was evaluated using Western blot. To evaluate the relationship among mitogen-activated protein kinases (MAPKs), activator protein 1 (AP-1), and FLG, we also treated cells with inhibitors for MAPKs JNK, p38, and ERK1/2. Results: FLG and FLG-2 mRNA expression in NHEKs significantly increased after treatment with $10{\mu}g/ml$ adiponectin. Adiponectin also restored FLG and FLG-2 mRNA expression that was otherwise inhibited by treatment with IL-4 and IL-13. Adiponectin induced FLG expression via AP-1 and MAPK signaling. Conclusion: Adiponectin positively regulated the expression of FLG and could be useful as a therapeutic agent to control diseases related to disrupted skin barrier function.

Keywords

Acknowledgement

Supported by : Ministry of Health & Welfare

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