생명과학회지 (Journal of Life Science)

  • 제28권8호
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  • Pages.969-976
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  • 2018
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Aspergillus niger가 생산하는 transglucosidase의 최적 생산 조건 확립

Establishment of Optimal Production Conditions of Transglucosidase Produced by Aspergillus niger

  • 이준엽 (국립안동대학교 생명과학과) ;
  • 강성호 (국립안동대학교 생명과학과) ;
  • 김종식 (국립안동대학교 생명과학과) ;
  • 정정욱 (국립안동대학교 생명과학과)
  • Lee, Jun-Yeob (Department of Biological Sciences, Andong National University) ;
  • Gang, Seongho (Department of Biological Sciences, Andong National University) ;
  • Kim, Jong-Sik (Department of Biological Sciences, Andong National University) ;
  • Chung, Chungwook (Department of Biological Sciences, Andong National University)
  • 투고 : 2018.06.09
  • 심사 : 2018.08.02
  • 발행 : 2018.08.30
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초록

본 연구에서는 Aspergillus niger 유래 TG의 최적 생산 조건을 연구하기 위해, A. niger 6 종을 이용하여 감자전분 1.5%, 효모추출물 1.0%, $KH_2PO_4$ 0.1%, 40%(v/v) volume의 배지를 기본 조성으로 하는 배지를 사용하였다. 기본 조성의 배지의 초기 pH를 달리하여 $30^{\circ}C$, 150 rpm의 조건으로 20일 동안 5일 간격으로 배양을 하여 TG 활성을 측정한 결과 초기 pH 5.0, 배양 15일, 그리고 A. niger KCTC6913 균주에서 가장 높은 TG 활성을 확인하였다. TG 생산에 유리한 탄소원, 질소원을 확립하기 위해 5 종의 다른 탄소원(감자 전분, 고구마 전분, 옥수수 전분, 밀 전분, 덱스트린) 또는 3종의 다른 질소원(효모추출물, 맥아추출물 및 쇠고기추출물)을 이용하여 초기 pH 5.0, 15일 배양한 후 TG 활성을 측정한 결과, 기본 조성 배지인 감자 전분 및 효모추출물에서 가장 높은 TG 활성을 확인 하였다. 또한 A. niger KCTC6913 균주는 호기적 조건 및 탄소원:질소원의 비율을 조절함에 따라 TG 생산이 증가함을 확인하였다. 확립된 최적 배지 및 배양 조건에서 TG 활성을 평가하기 위해 기본배지 및 A. niger 배양에 널리 이용되는 PDB 배지를 대조군으로 하여 비교한 결과 기본 조성의 배지에 비해 1.3배, PDB 배지에 비해 4배 높은 TG 활성을 확인 하였다. 종합적으로 이러한 연구결과는 A. niger 유래의 TG를 산업적으로 이용하기 위한 최적 배양 배지 및 배양 조건을 성공적으로 수립하였음을 시사한다.

In this study, transglucosidase (TG), an enzyme produced by Aspergillus niger, synthesized isomaltooligosaccharide from ${\alpha}-(1{\rightarrow}4)$ linked substrates. The highest TG-producing A. niger KCTC6913 was selected from six kinds of species, and optimized TG producing conditions were established. Five different carbon sources (potato starch, sweet potato starch, corn starch, wheat starch, and dextrin) and three different nitrogen sources (yeast extract, malt extract, and beef extract) were tested to establish the carbon and nitrogen sources favorable for TG production. Measurements of TG activity after an initial culture at pH 5.0 for 15 days revealed that potato starch and yeast extract, which are basic culture media, resulted in the highest TG activity. In addition, A. niger KCTC6913 increased TG production under aerobic conditions and a controlled carbon/nitrogen ratio. In conclusion, to evaluate TG activity in the established optimal medium, it is confirmed that the basal and potato dextrose broth medium were used as a control, and the highest TG production was measured, which was highlighted in the established optimal medium.

키워드

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