생명과학회지 (Journal of Life Science)

  • 제28권8호
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  • Pages.938-944
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  • 2018
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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꼬리진달래 발효추출물의 이화학적 특성 및 생리활성 연구

Physicochemical Characterization of Fermented Rhododendron micranthum Turcz. Extract and Its Biological Activity

  • 김민진 (국립낙동강 생물자원관 담수생물특성연구실) ;
  • 유상미 (국립낙동강 생물자원관 담수생물특성연구실) ;
  • 김도연 ((재)금산국제인삼약초연구소 기술사업부) ;
  • 허태임 (국립백두대간수목원 산림식물산업부실) ;
  • 이준우 (국립백두대간수목원 산림식물산업부실) ;
  • 박지애 (국립백두대간수목원 산림식물산업부실) ;
  • 박창수 (대구가톨릭대학교 식품가공학전공) ;
  • 김영수 (국립백두대간수목원 산림식물산업부실)
  • Kim, Min-Jin (Freshwater Bioresources Utilization Division, Nakdonggang National Institute of Biological Resource) ;
  • Yu, Sang-Mi (Freshwater Bioresources Utilization Division, Nakdonggang National Institute of Biological Resource) ;
  • Kim, Do-Yeon (Department of Pharmaceutical Science and Technology, International Ginseng and Herb Research Institute) ;
  • Heo, Tae-Im (Forest Plant Industry Department, Baekdudaegan National Arboretum) ;
  • Lee, Jun Woo (Forest Plant Industry Department, Baekdudaegan National Arboretum) ;
  • Park, Ji-Ae (Forest Plant Industry Department, Baekdudaegan National Arboretum) ;
  • Park, Chang-Su (Department of Food Science and Technology, Daegu Catholic University) ;
  • Kim, Yeong-Su (Forest Plant Industry Department, Baekdudaegan National Arboretum)
  • 투고 : 2018.03.22
  • 심사 : 2018.04.03
  • 발행 : 2018.08.30
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초록

우리나라 특산, 희귀 식물로 알려져 있는 꼬리진달래(Rhododendron micranthum Turcz)를 기능성 유산균 Lactobacillus rhamnosus를 이용하여 발효 소재를 만들고 발효 소재의 항산화 활성 및 피부 개선 활성을 조사 하였다. 고형분 함량 3%의 꼬리진달래 추출물에 L. rhamnosus를 3%가 되게 접종하여 3일간 배양하여 $5{\times}10^9CFU/ml$의 높은 농도의 유산균이 배양 되었고 총페놀 함량은 배양 전 130 GAE mg/ml에서 157 GAE mg/ml로 증가 한 것으로 확인 되었다. DPPH, ABTS radical 소거능과 tyrosinase, elastase 저해 활성은 발효 전과 비교 하였을 때 각각 1.3, 1.5, 2.4, 5.6배 높게 나타났다. 이러한 결과는 꼬리진달래 추출물을 유산균을 이용 하여 발효하였을 때 새로운 발효소재로 식품 및 화장품 산업 분야에 다양한 용도로 사용 가능할 것으로 생각 된다. 하지만 발효에 의한 천연 성분의 변화 및 활성 성분에 대한 분석, 생리활성 관련 작용 기전에 대해 추가적인 연구가 진행되어야 할 것이다.

This study evaluated tyrosinase, elastase inhibitory, and antioxidant activities of fermented Rhododendron micranthum Turcz. extract using a lactic acid bacterium, Lactobacillus rhamnosus. The optimum conditions for fermentation of R. micranthum Turcz. extract were $37^{\circ}C$ and 3% R. micranthum Turcz. extract for 3 days based on the bacterial cell number, total phenolic compounds, DPPH radical scavenging activity, and tyrosinase and elastase inhibitory activity. After culture for 3 days using 3% R. micranthum Turcz. extract, the cell mass of L. rhamnosus reached $5.7{\times}10^9CFU/ml$. The results indicated that R. micranthum Turcz. extract can be used for industrial lactic acid bacteria culture. After fermentation under optimum conditions, the total content of phenolic compounds of the fermented R. micranthum Turcz. extract was 157 GAE mg/ml, and the $IC_{50s}$ of DPPH radical scavenging activity, ABTS radical scavenging activity, and tyrosinase and elastase inhibitory effects were 78.8, 79.8, 329.1, and $449.5{\mu}g/ml$, respectively. The fermented R. micranthum Turcz. extract exhibited 1.2-, 1.3-, 1.5-, 2.4-, and 5.6-fold higher total content of phenolic compounds, DPPH radical scavenging activity, ABTS radical scavenging activity, and tyrosinase and elastase inhibitory effects than the nonfermented R. micranthum Turcz. extract. These results indicated that fermented R. micranthum Turcz. extract using L. rhamnosus can be used for developing new natural functional ingredients for the health food or cosmetic industry.

키워드

참고문헌

  1. Blois, M. S. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181, 1199-1203. https://doi.org/10.1038/1811199a0
  2. Choi, M. O., Kim, B. J., Jo, S. K., Jung, H. K., Lee, J. T., Kim, H. Y. and Kweon, D. J. 2013. Anti-allergic activities of Castanea crenata inner shell extracts fermented by Lactobicillus bifermentans. Kor. J. Food Preserv. 20, 583-591. https://doi.org/10.11002/kjfp.2013.20.4.583
  3. Choi, W. S., Kwon, H. S., Lim, H. W., No, R. W. and Lee, H. Y. 2013. Whitening effects of Lactobacillus rhamnosus associated with its antioxidative activities. Kor. J. Microbiol. Biotechnol. 41, 183-189. https://doi.org/10.4014/kjmb.1302.02006
  4. Diep, D. B., Godager, L., Brede, D. and Nes, I. F. 2006. Datamining and characterization of a novel pediocin-like bacteriocin system from the genome of Pediococcus pentosaceus ATCC 25745. Microbiology 152, 1649-1659. https://doi.org/10.1099/mic.0.28794-0
  5. Doron, S., Snydman, D. R. and Gorbach, S. L. 2005. Lactobacillus GG: Bacteriology and clinical applications. Gastroenterol. Clin. North Am. 34, 483-498. https://doi.org/10.1016/j.gtc.2005.05.011
  6. Ha, Y. J., Yoo, S. K. and Kim, M. R. 2016. Process optimization of ginseng berry extract fermentation by Lactobacillus sp. strain KYH isolated from fermented Kimchi and product analysis. J. East Asian Soc. Diet. Life 26, 88-98. https://doi.org/10.17495/easdl.2016.2.26.1.88
  7. Hur, S. J., Lee, S. Y., Kim, Y. C. Choi, I. and Kim, G. B. 2014. Effect of fermentation on the antioxidant activity in plant based foods. Food Chem. 160, 346-356. https://doi.org/10.1016/j.foodchem.2014.03.112
  8. JI, G. E. 1994. Composition and distribution of intestinal microbial flora in Korean. Kor. J. Appl. Microbiol. Biotechnol. 22, 453-458.
  9. Kang, C. H., Kim, S. C., Jeong, S. C., Han, W., Lee, S. Y., Yu, S. M., Jin, H. M. and Kim, Y. S. 2016. Physicochemical characteristics of fermented Phragmites communis extract and its biological activity. Kor. J. Phamacogn. 47, 273-279.
  10. Kang, D. H., Kim, J. W. and Youn, K. S. 2011. Antioxidant activities of extract from fermented mulberry (Cudrania tricuspidata) fruit and inhibitory action on elastase and tyrosinase. Kor. J. Food Preserv. 18, 236-243. https://doi.org/10.11002/kjfp.2011.18.2.236
  11. Kim, N. Y., Bae, K. H., Kim, Y. S., Lee, H. B. and Park, W. G. 2013. Habitat environment and cutting, seed propagation of rare plant Rhododendron micranthum Turcz. J. For. Environ. Sci. 29, 165-172.
  12. Kim, N. Y., Kim, H. S., Kim, Y. S. and Park, W. G. 2006. Studies on morphological variation among provenances of a rare Rhododendron micranthum in Korea. J. Kor. For. Soc. 95, 55-59.
  13. Kim, N. Y., Park, D. S. and Lee, H. Y. 2015. Effect of antiskin wrinkle and antioxidant of Agastache rugosa Kentz through fermentation process of the lactic acid. Kor. J. Medicinal Crop Sci. 23, 37-42. https://doi.org/10.7783/KJMCS.2015.23.1.37
  14. Kim, Y. S., Lee, H., Kim, D. Y., Kim, S. Y., Lee, W. K., Lee, S. M., Park, J. D. and Shon, M. Y. 2013. Cultivation of lactic acid bacteria for the development of probiotic products using red ginseng starch. J. East Asian Soc. Diet. Life 23, 818-826.
  15. Lee, Y. W., Lee, J. E., Kim, J. H., Yeo, S. H., Back, S. Y., Park, S. Y. and Park, H. Y. 2013. Status of technology on fermented liquid. Food Sci. Ind. 46, 33-47.
  16. Mathara, J. M., Schillinger, U., Guigas, C., Franz, C. M. A. P., Kutima, P. M., Mbugua, S., Shin, H. K. and Holzapfel, W. H. 2008. Functional characteristics of Lactobacillus sp. from traditional maassai fermented milk products in Kenya. Int. J. Food Microbiol. 126, 57-64. https://doi.org/10.1016/j.ijfoodmicro.2008.04.027
  17. Muller, D. M., Carrasco, M. S., Tonarelli, G. G. and Simonetta, A. C. 2009. Characterization and purification of a new bacteriocin with a broad inhibitory spectrum produced by Lactobacillus plantarum Ip 31 strain isolated from dry-fermented sausage. J. Appl. Microbiol. 106, 2031-2040. https://doi.org/10.1111/j.1365-2672.2009.04173.x
  18. Park, K. Y. 2012. Increased health functional of fermented foods. Food Ind. Nutr. 17, 1-8.
  19. Park, S. H., Yang, S., Lee, J. H. and Kang, M. 2013. Selection of phytate-degrading lactic acid bacteria from Kimchi and reaction properties in brown rice. J. Kor. Soc. Food Sci. Nutr. 42, 627-632. https://doi.org/10.3746/jkfn.2013.42.4.627
  20. Park, Y. S. and Jang, H. G. 2003. Evaluation of physiological activity and lactic acid fermentation of Rubus Coreanus Miq. J. Kor. Soc. Agric. Chem. 46, 367-375.
  21. Pellegrini, N., Re, R., Yang, M. and Rice-Evans, V. 1998. Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis (3-ethylenebenzothiazoline-6-sulfonic acid) radical cationdecol orization assay. Meth. Enzymol. 299, 379-389.
  22. Prince, T., McBain, A. J. and O'Neill, C. A. 2012. Lactobacillus reuteri protects epidermal keratinocytes from Staphylococcus aureus-induced cell death by competitive exclusion. Appl. Environ. Microbiol. 78, 5119-5126. https://doi.org/10.1128/AEM.00595-12
  23. Rajan, K. N. and Rajendan, A. D. 2009. Effect of fermentation parameters on extra cellular tannase production by Lactobacillus plantarum MTCC 1407. Eur. J. Chem. 6, 979-984.
  24. Shin, J. H. and Yoo, S. K. 2012. Antioxidant properties in microbial fermentation products of Lonicera japonica Thunb. extract. J. East Asian Soc. Diet. Life 22, 95-102.
  25. Wee, Y. J., Kim, H. O., Yun, J. S. and Ryu, H. W. 2006. Pilot-scale lactic acid production via batch culturing of Lactobacillus sp. RKY2 using corn steep liquor as a nitrogen source. Food Technol. Biotechnol. 44, 293-298.