생명과학회지 (Journal of Life Science)

  • 제28권8호
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  • Pages.923-930
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  • 2018
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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유용 미생물을 이용한 발효갈색거저리 추출물의 이화학적 특성 및 생리활성 효과

Physicochemical Properties and Biological Activities of Tenebrio molitor Fermented by Several Kinds of Micro-organisms

  • 투고 : 2018.03.16
  • 심사 : 2018.04.11
  • 발행 : 2018.08.30
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초록

본 연구는 미생물을 이용하여 갈색거저리를 발효시킨 분말을 water, ethanol, methanol 용매별로 추출하여 다양한 실험을 행하였다. 균주는 3종의 유산균 Lactobacillus plantarum JBMI F3 (F3), Lactobacillus plantarum JBMI F5 (F5), Lactobacullus gasseri Ba9 (Ba9), 1종의 곰팡이 Aspergillus kawachii KCCM 32819 (Ak), 1종의 효모 Saccharomyces cerevisiae KACC 93023 (Sc), 1종의 바실러스 Bacillus subtilis KACC 91157 (Bs) 총 6종의 균주를 사용하였다. 각 균주를 이용한 발효 후 갈색거저리의 효능을 알아보기 위해 DPPH assay, 총 phenolic compound 및 Flavonoid 함량, Cu 환원력, Fibrinolytic activity, 효소-기질 활성 분석을 진행하였다. 그 결과, DPPH assay 결과, Water 추출물이 전반적으로 높은 항산화능을 보였고, 그 중 Bs 균주 발효 추출물이 우수한 라디컬 소거능을 보였다. 총 phenolic compound 및 Flavonoid의 경우, phenolic compound는 Ak발효군이 가장 함량이 많았고, Flavonoid는 Bs발효군이 가장 높았다. 환원력은 ethanol - Bs발효군에서 높은 수치를 확인하였고, Fibrinolytic activity 및 전분분해능은 water - Bs발효군에서 우수한 효과를 나타냈다. 위와 같은 결과를 토대로 발효 갈색거저리분말은, 기존 갈색거저리 분말보다 항산화능 및 생리활성 측면에서 효능이 상당부분 향상됨을 알 수 있었다. 그러므로 발효거저리분말은 식품산업전반에서 높은 이용가치를 증명하는 기초 연구자료로 활용될 것이다.

In this study, Tenebrio molitor (T. molitor) was fermented with Lactobacillus plantarum JBMI F3 (F3), Lactobacillus plantarum JBMI F5 (F5), Lactobacillus gasseri Ba9 (Ba9), Aspergillus kawachii KCCM 32819 (Ak), Saccharomyces cerevisiae KACC 93023 (Sc), and Bacillus subtilis KACC 91157 (Bs). After fermentation, the fermented products were extracted by water, ethanol, and methanol, and their physicochemical and biological properties were investigated. In a DPPH assay, the water extracts of the fermented products of T. molitor showed high antioxidant ability. Among the water extracts, the fermented product by Bs showed the highest DPPH radical scavenging activity. The total contents of phenolic compounds and flavonoids were highest in the fermented products by Ak and Bs, respectively. Reducing activity was detected the most high activity on ethanol extract of fermented product by Bs. The water extract of the fermented product by Bs exhibited strong enzymatic activity for fibrinogen and starch hydrolysis. Based on the observed physicochemical and biological properties, the fermented products of T. molitor by microorgansims can likely be applied as functional materials in various industries.

키워드

참고문헌

  1. An, S. I. 2005. Antioxidative and physiological activities of some phenolic compounds. Department of Food and Biotechnology Graduate School of Technology Hankyong National University.
  2. Astrup, T. and Mullertz, S. 1991. The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem. Biophys. 40, 346-351.
  3. Bijoy, M., Jayati, S. and Prabir, K. Sarkar. 2008. Antioxidant activities of soybean as affected by Bacillus-fermentation to kinema. Food Res. Int. 41, 586-593 https://doi.org/10.1016/j.foodres.2008.04.003
  4. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1204.
  5. Chang, H. G. and Park, Y. S. 2003. Lactic acid fermentation and biological activities of Rubus coreanus. J. Appl. Biol. Chem. 46, 367-375.
  6. Chung, S. J., Lee, Y. H., Chung, J. H., Lee, B. R. and Han, D. M. 1995. Antifungal Effect and activity spectrum of crude antifungal proteins from hemolymph of larvae of Tenebrio molitor in Korea. Hanguk Kyun Hakoe Chi 23, 232-237.
  7. Dibyangana, R., Tania, B., Suchita, M., Shrayan, K. D. and Suvroma, G. 2014. Production and partial purification of alpha amylase from Bacillus subtilis (MTCC 121) using solid state fermentation. Biochem. Res. Int. 2014, 568141.
  8. Duncan, D. B. 1955. Multiple range and multiple F test. Biometrics 1, 1-42.
  9. Ahn, H. Y., Choe, D. J., Kim, B. K., Lee, J. H. and Cho, Y. S. 2015. Bioactive materials and antioxidant properties of fermented rice-bran extract. Saengmyeong Gwahag Hoeji 25, 1014-1020.
  10. Huang, M. X., Ye, Y., Chen, Y. and Han, Y. L. 2012. Partial purification and characterization of fibrinolytic enzymes from yellow mealworm. Int. J. Pept. Res. Ther. 18, 153-161. https://doi.org/10.1007/s10989-012-9288-x
  11. Cha, J. Y., Kim, Y. S., Ahn, H. Y., Kang, M. J., Heo, S. J. and Cho, Y. S. 2011. Biological activity and biochemical properties of silkworm (Bombyx mori L.) powder fermented with Bacillus subtilis and Aspergillus kawachii. Saengmyeong Gwahag Hoeji 21, 81-88.
  12. Jeong, M. E., Kang, S. A. and Cheong, C. 2014. Physicochemical characteristics of the mash quality of Cheongju prepared using different Nuruks. JKAIS. 15, 5150-5158.
  13. Jia, Z., Tang, M. and Wu, J. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64, 555-559 https://doi.org/10.1016/S0308-8146(98)00102-2
  14. Lee, J. H., Um, Y. R., Shim, K. S., Jeon, W. K., Lee, J. H. and Ma, J. Y. 2009. Acute toxicity study on fermented Ssanghwa-tang extracts in mice. Kor. J. Orient. Int. Med. 30, 780-787.
  15. Kaur, C. and Kapoor, H. C. 2002. Anti-oxidant activity and total phenolic content of some Asian vegetables. Int. J. Food Sci. Technol. 37, 153-161. https://doi.org/10.1046/j.1365-2621.2002.00552.x
  16. Kim, H. A., Lee, S. H., Choi, Y. C., Park, K. H., Hwang, J. S., Kim, N. J. and Nam, S. H. 2013. Comparison of fibrinolytic activity from Korean indigenous insects. J. Seric. Entomol. Sci. 51, 147-152.
  17. Kim, S. Y., Son, Y. J., Kim, S. H., Kim, A. N., Lee, G. Y. and Hwang, I. K. 2015. Studies on oxidative stability of Tenebrio molitor Larvae during cold storage. Kor. J. Food Cook Sci. 31, 62-71 https://doi.org/10.9724/kfcs.2015.31.1.062
  18. Chae, K. S., Jung, J. H., Yoon, H. H. and Son, R. H. 2014. Antioxidant activity and main volatile flavor components of mulberry wine fermented with Saccharomyces cerevisiae B-8. J. Kor. Soc. Food Sci. Nutr. 43, 1017-1024. https://doi.org/10.3746/jkfn.2014.43.7.1017
  19. Lee, S. Y., Kim, J. H., Park, J. M., Lee, I. C. and Lee, J. Y. 2014. Antioxidant activity and inhibition activity against ${\alpha}$-amylase and ${\alpha}$-glucosidase of Smilax China L. Kor. J. Food Preserv. 21, 254-263. https://doi.org/10.11002/kjfp.2014.21.2.254
  20. Liu, S., Sun, J., Yu, L., Zhang, C., Bi, J., Zhu, F. and Yang, Q. 2012. Antioxidant activity and phenolic compounds of Holotrichia parallela Motschulsky extracts. Food Chem. 134, 1885-1891. https://doi.org/10.1016/j.foodchem.2012.03.091
  21. Baek, M. H., Seo, M. C., Kim, M. A., Yun, E. Y. and Hwang, J. S. 2017. The antioxidant activities and hair-growth promotion effects of Tenebrio molitor Larvae extracts (TMEs). Saengmyeong Gwahag Hoeji 27, 1269-1275.
  22. Kang, M. S., Kim, M. J., Han, J. S. and Kim, A. J. 2017. Fatty acid composition and anti-inflammatory effects of the freeze dried Tenebrio molitor Larva. Kor. J. Food Nutr. 30, 251-256. https://doi.org/10.9799/ksfan.2017.30.2.251
  23. Oomah, B. D., Cardador‐Martinez, A. and Loarca‐Pina, G. 2005. Phenolics and antioxidative activities in common beans (Phaseolus vulgaris L). J. Sci. Food Agric. 85, 935-942. https://doi.org/10.1002/jsfa.2019
  24. Phonesavanh, P., Kim, K. K., Lim, J. C., Lee, K. Y., Park, C. H. and Choe, M. 2015. Saccharification of Fagopyrum esculentum by amylase treatments increases phenolic compound content and antioxidant activity. J. East Asian Soc. Diet Life 25, 139-145. https://doi.org/10.17495/easdl.2015.2.25.1.139
  25. Hwang, S. Y., Bae, G. K. and Choi, S. K. 2015. Preferences and purchase intention of Tenebrio molitor (Mealworm) according to cooking method. Culi. Sci. Hos. Res. 21, 100-115.
  26. Swain, T. and Hillis, W. E. 1959. The phenolic constituents of Prunus domestica. I. The quantitative analysis of phenolic constituents. J. Sci. Food Agric. 10, 63-68. https://doi.org/10.1002/jsfa.2740100110
  27. Yoo, J. M., Hwang, J. S., Goo, T. W. and Yun, E. Y. 2013. Comparative analysis of nutritional and harmful components in Korean and Chinese mealworms (Tenebrio molitor). J. Kor. Soc. Food Sci. Nutr. 42, 249-254. https://doi.org/10.3746/jkfn.2013.42.2.249