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Enhancing Electricity Generation Using a Laccase-Based Microbial Fuel Cell with Yeast Galactomyces reessii on the Cathode

  • Chaijak, Pimprapa (Department of Biotechnology, Faculty of Science, Thaksin University, Phatthalung Campus) ;
  • Sukkasem, Chontisa (Department of Food Science and Technology, Faculty of Technology and Community Development, Thaksin University, Phatthalung Campus) ;
  • Lertworapreecha, Monthon (Department of Biology, Faculty of Science, Thaksin University, Phatthalung Campus) ;
  • Boonsawang, Piyarat (Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hatyai Campus) ;
  • Wijasika, Sutthida (Department of Biotechnology, Faculty of Technology and Community Development, Thaksin University, Phatthalung Campus) ;
  • Sato, Chikashi (Department of Civil and Environmental Engineering, College of Science and Engineering, Idaho State University)
  • Received : 2018.03.13
  • Accepted : 2018.06.11
  • Published : 2018.08.28

Abstract

The fungi associated with termites secrete enzymes such as laccase (multi-copper oxidase) that can degrade extracellular wood matrix. Laccase uses molecular oxygen as an electron acceptor to catalyze the degradation of organic compounds. Owing to its ability to transfer electrons from the cathodic electrode to molecular oxygen, laccase has the potential to be a biocatalyst on the surface of the cathodic electrode of a microbial fuel cell (MFC). In this study, a two-chamber MFC using the laccase-producing fungus Galactomyces reessii was investigated. The fungus cultured on coconut coir was placed in the cathode chamber, while an anaerobic microbial community was maintained in the anode chamber fed by industrial rubber wastewater and supplemented by sulfate and a pH buffer. The laccase-based biocathode MFC (lbMFC) produced the maximum open circuit voltage of 250 mV, output voltage of 145 mV (with a $1,000{\Omega}$ resistor), power density of $59mW/m^2$, and current density of $278mA/m^2$, and a 70% increase in half-cell potential. This study demonstrated the capability of laccase-producing yeast Galactomyces reessii as a biocatalyst on the cathode of the two-chamber lbMFC.

Keywords

References

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