The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Anti-Inflammatory Effects of Streamed Platycodon grandiflorum against UVB Radiation-Induced Oxidative Stress in Human Primary Dermal Fibroblast

  • Lee, Ji Yeon (Dept. of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Park, Jeong-Yong (Dept. of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Lee, Dae Young (Dept. of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Kim, Hyung Don (Dept. of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Kim, Geum-Soog (Dept. of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Lee, Seung Eun (Dept. of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Seo, Kyung Hye (Dept. of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
  • Received : 2018.05.18
  • Accepted : 2018.07.27
  • Published : 2018.08.31
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Abstract

Ultraviolet B (UVB) exposure is a risk factor for skin damage resulting in oxidative stress, inflammation, and cell death. The purpose of this study was to investigate the physicochemical properties of Platycodon grandiflorum (PG) to improve its biological activities using a three-step steaming process. We investigated the protective effects of PG and steamed PG extracts on human dermal fibroblasts (HDFs) against UVB radiation-induced oxidative stress and inflammation as well as the underlying mechanisms. The antioxidant potential of the PG extracts was evaluated by measuring the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) scavenging activity. ABTS and DPPH were shown by the 0, 30, and 70% ethanol extracts of 2S-PG and 3S-PG ($IC_{50}$, 28~45 and $27{\sim}30{\mu}g/mL$, respectively). Treatment of UVB-irradiated cells with steamed PG ($25{\sim}400{\mu}g/mL$) did not affect their viability. The streamed PG extract suppressed UVB-induced generation of reactive oxygen species (ROS). In addition, streamed PG extract reduced cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) protein expression in UVB-irradiated HDF, regulating nuclear factor $(NF)-{\kappa}B$ expression. These findings suggest that steamed PG extract may be potentially effective against inflammation associated with UVB-induced oxidation stress.

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References

  1. Akhalaya MY, Maksimov G, Rubin AB, Lademann J, Darvin ME. 2014. Molecular action mechanisms of solar infrared radiation and heat on human skin. Ageing Res Rev 16:1-11 https://doi.org/10.1016/j.arr.2014.03.006
  2. Berneburg M, Plettenberg H, Krutmann J. 2000. Photoaging of human skin. Photodermatol, Photoimmunol Photomed 16:239-244 https://doi.org/10.1034/j.1600-0781.2000.160601.x
  3. Brand-Williams W, Cuvelier ME, Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Science Technol 28:25-30 https://doi.org/10.1016/S0023-6438(95)80008-5
  4. Cho YS. 2011. Characteristics of seedling establishment and yield of Platycodon grandiflorus by ridge width and mulching materials. Korean J Med Crop Sci 19:233-237 https://doi.org/10.7783/KJMCS.2011.19.4.233
  5. Clydesdale GJ, Dandie GW, Muller HK. 2001. Ultraviolet light induced injury: Immunological and inflammatory effects. Immunol Cell Biol 79:547-568 https://doi.org/10.1046/j.1440-1711.2001.01047.x
  6. Ghosh M, Das J, Sil PC. 2012. D(+) galactosamine induced oxidative and nitrosative stress-mediated renal damage in rats via NF-${\kappa}$B and inducible nitric oxide synthase (iNOS) pathways is ameliorated by a polyphenol xanthone, mangiferin. Free Radical Res 46:116-132 https://doi.org/10.3109/10715762.2011.644240
  7. Jang MH, Kim CJ, Kim EH, Kim MG, Leem KH, Kim J. 2006. Effects of Platycodon grandiflorum on lipopolysaccharidestimulated production of prostaglandin E2, nitric oxide, and interleukin-8 in mouse microglial BV2 cells. J Med Food 9:169-174 https://doi.org/10.1089/jmf.2006.9.169
  8. Karas M, Baraniak B, Rybczynska K, Gminski J, Gawel-Beben K, Jakubczyk A. 2015. The influence of heat treatment of chickpea seeds on antioxidant and fibroblast growth-stimulating activity of peptide fractions obtained from proteins digested under simulated gastrointestinal conditions. Int J Food Sci Technol 50:2097-2103 https://doi.org/10.1111/ijfs.12872
  9. Kim GS, Lee DY, Lee SE, Noh HJ, Choi JH, Park CG, Choi SI, Hong SJ, Kim SY. 2013. Evaluation on extraction conditions and HPLC analysis method for bioactive compounds of Astragali radix. Korean J Med Crop Sci 21:486-492 https://doi.org/10.7783/KJMCS.2013.21.6.486
  10. Kim SY, Lee YJ, Park DS, Kim HR, Cho YS. 2015. Comparison of quality characteristics of Platycodon grandiflorum according to steaming and fermentation. Korean J Food Preserv 22:851-858 https://doi.org/10.11002/kjfp.2015.22.6.851
  11. Kuo YH, Wu PY, Chen CW, Lin P, Wen KC, Lin CY, Chiang HM. 2017. N-(4-bromophenethyl) caffeamide protects skin from UVB-induced inflammation through MAPK/IL-6/NF-${\kappa}$ B-dependent signaling in human skin fibroblasts and hairless mouse skin. Molecules 22:1639-1652 https://doi.org/10.3390/molecules22101639
  12. Lee BJ, Jeon SH, No IR, Kim YG, Cho YS. 2015. Effect of saponin content and antioxidant activities of Platycodon grandiflorum radix by cutting length. Korean J Med Crop Sci 23:363-369 https://doi.org/10.7783/KJMCS.2015.23.5.363
  13. Lee SH, Ha SE, Cho HK, Park JK. 2014. Inhibitory effects of ethanol extracts of some Korean plants on ultraviolet B-induced DNA damage. Korean J Food Nutr 27:845-850 https://doi.org/10.9799/ksfan.2014.27.5.845
  14. Lee JY, Hwang WI, Lim ST. 2004. Antioxidant and anticancer activities of organic extracts from Platycodon grandiflorum A. De Candolle roots. J Ethnopharmacology 93:409-415 https://doi.org/10.1016/j.jep.2004.04.017
  15. Lee KJ, Choi CY, Chung YC, Kim YS, Ryu SY, Roh SH, Jeong HG. 2004. Protective effect of saponins derived from roots of Platycodon grandiflorum on tert-butyl hydroperoxideinduced oxidative hepatotoxicity. Toxicol Lett 147:271-282 https://doi.org/10.1016/j.toxlet.2003.12.002
  16. Lee KJ, You HJ, Park SJ, Kim YS, Chung YC, Jeong TC, Jeong HG. 2001. Hepatoprotective effects of Platycodon grandiflorum on acetaminophen-induced liver damage in mice. Cancer Lett 174:73-81 https://doi.org/10.1016/S0304-3835(01)00678-4
  17. Lee SJ, Shin SR, Yoon KY. 2013. Physicochemical properties of black Doraji (Platycodon grandiflorum). Korean J Food Sci Technol 45:422-427 https://doi.org/10.9721/KJFST.2013.45.4.422
  18. Lim J, Tae I, Lee B, Kim H. 2016. Protective effect of Platycodon grandiflorum extract against in UVB-induced oxidative stress in keratinocytes. Toxicol Lett 258:298-299
  19. Oh YC, Kang OH, Choi JG, Lee YS, Brice OO, Jung HJ, Hong SH, Lee YM, Shin DW, Kim YS, Kwon DY. 2010. Anti-allergic activity of a platycodon root ethanol extract. Int J Mol Sci 11:2746-2758 https://doi.org/10.3390/ijms11072746
  20. Pillai S, Oresajo C, Hayward J. 2005. Ultraviolet radiation and skin aging: Roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation - A review. Int J Cosmet Sci 27:17-34 https://doi.org/10.1111/j.1467-2494.2004.00241.x
  21. Radhiga T, Agilan B, Muzaffer U, Karthikeyan R, Kanimozhi G, Paul V, Prasad NR. 2016. Phytochemicals as modulators of ultraviolet-b radiation induced cellular and molecular events - A review. J Radiat Cancer Res 7:2-12 https://doi.org/10.4103/0973-0168.184607
  22. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med 26:1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  23. Ryu HS. 2014. Effects of water extract from Platycodon grandiflorum on mouse immune cell activation ex vivo by oral administration. Korean J Food Nutr 27:99-104 https://doi.org/10.9799/ksfan.2014.27.1.099
  24. Serasanambati M, Chilakapati SR. 2016. Function of nuclear factor kappa B (NF-kB) in human diseases - A review. South Indian J Biol Sci 2:368-387 https://doi.org/10.22205/sijbs/2016/v2/i4/103443
  25. Tada A, Kaneiwa Y, Shoji J, Shibata S. 1975. Studies on the saponins of the root of Platycodon grandiflorum A. De Candolle. I. Isolation and the structure of platycodin-D. Chem Pharm Bull 23:2965-2972 https://doi.org/10.1248/cpb.23.2965

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