Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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Bacterial leakage and micro-computed tomography evaluation in round-shaped canals obturated with bioceramic cone and sealer using matched single cone technique

  • Received : 2018.01.21
  • Accepted : 2018.05.27
  • Published : 2018.08.31
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Abstract

Objectives: To evaluate sealing ability of root canals obturated with bioceramic-impregnated gutta percha cone (BCC) or gutta percha (GP), with bioceramic sealer (BCS) or AH Plus (AH; Dentsply-Maillefer), in roundly-prepared canals using matched single-cone technique, based on bacterial leakage test, and to analyze obturation quality using micro-computed tomography (CT) analysis. Materials and Methods: Ninety-two distobuccal roots of maxillary molars were prepared using nickel-titanium files to apical size 40/0.06. The roots were divided into 4 groups (n = 20) that were obturated with a master cone and sealer: GP/AH, BCC/AH, GP/BCS, and BCC/BCS. Bacterial leakage model using Enterococcus faecalis was used to evaluate sealing ability for 60-day period. Obturated samples from each group (n = 4) were analyzed using micro-CT. Results: All groups showed bacterial leakage at 20%-45% of samples with mean leakage times of 42-52 days. There were no significant differences in bacterial leakage among the groups. Micro-CT showed minimal gaps and voids in all groups at less than 1%. Conclusions: In roundly-prepared canals, the single cone obturation with BCC/BCS was comparable to GP/AH for bacterial leakage at 60 days.

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References

  1. Sundqvist G, Figdor D, Persson S, Sjogren U. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998;85:86-93. https://doi.org/10.1016/S1079-2104(98)90404-8
  2. Zhang W, Li Z, Peng B. Assessment of a new root canal sealer's apical sealing ability. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;107:e79-e82. https://doi.org/10.1016/j.tripleo.2009.02.024
  3. Zhang W, Li Z, Peng B. Ex vivo cytotoxicity of a new calcium silicate-based canal filling material. Int Endod J 2010;43:769-774. https://doi.org/10.1111/j.1365-2591.2010.01733.x
  4. Zoufan K, Jiang J, Komabayashi T, Wang YH, Safavi KE, Zhu Q. Cytotoxicity evaluation of Gutta Flow and Endo Sequence BC sealers. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011;112:657-661. https://doi.org/10.1016/j.tripleo.2011.03.050
  5. Willershausen I, Callaway A, Briseno B, Willershausen B. In vitro analysis of the cytotoxicity and the antimicrobial effect of four endodontic sealers. Head Face Med 2011;7:15. https://doi.org/10.1186/1746-160X-7-15
  6. Zhou HM, Shen Y, Zheng W, Li L, Zheng YF, Haapasalo M. Physical properties of 5 root canal sealers. J Endod 2013;39:1281-1286. https://doi.org/10.1016/j.joen.2013.06.012
  7. Zhang H, Shen Y, Ruse ND, Haapasalo M. Antibacterial activity of endodontic sealers by modified direct contact test against Enterococcus faecalis. J Endod 2009;35:1051-1055. https://doi.org/10.1016/j.joen.2009.04.022
  8. Chotvorrarak K, Yanpiset K, Banomyong D, Srisatjaluk R. In vitro antibacterial activity of oligomer-based and calcium silicate-based root canal sealers. Mahidol Dent J 2017;37:145-154.
  9. Adib V, Spratt D, Ng YL, Gulabivala K. Cultivable microbial flora associated with persistent periapical disease and coronal leakage after root canal treatment: a preliminary study. Int Endod J 2004;37:542-551. https://doi.org/10.1111/j.1365-2591.2004.00840.x
  10. Timpawat S, Amornchat C, Trisuwan WR. Bacterial coronal leakage after obturation with three root canal sealers. J Endod 2001;27:36-39.
  11. Shipper G, Trope M. In vitro microbial leakage of endodontically treated teeth using new and standard obturation techniques. J Endod 2004;30:154-158. https://doi.org/10.1097/00004770-200403000-00007
  12. Pinheiro CR, Guinesi AS, de Camargo EJ, Pizzolitto AC, Filho IB. Bacterial leakage evaluation of root canals filled with different endodontic sealers. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;108:e56-e60.
  13. Jafari F, Jafari S. Importance and methodologies of endodontic microleakage studies: a systematic review. J Clin Exp Dent 2017;9:e812-e819.
  14. Verissimo DM, do Vale MS. Methodologies for assessment of apical and coronal leakage of endodontic filling materials: a critical review. J Oral Sci 2006;48:93-98. https://doi.org/10.2334/josnusd.48.93
  15. Wu MK, Wesselink PR. Endodontic leakage studies reconsidered. Part I. Methodology, application and relevance. Int Endod J 1993;26:37-43. https://doi.org/10.1111/j.1365-2591.1993.tb00540.x
  16. Celikten B, Uzuntas CF, Orhan AI, Tufenkci P, Misirli M, Demiralp KO, Orhan K. Micro-CT assessment of the sealing ability of three root canal filling techniques. J Oral Sci 2015;57:361-366. https://doi.org/10.2334/josnusd.57.361
  17. Celikten B, Uzuntas CF, Orhan AI, Orhan K, Tufenkci P, Kursun S, Demiralp KO. Evaluation of root canal sealer filling quality using a single-cone technique in oval shaped canals: an in vitro Micro-CT study. Scanning 2016;38:133-140. https://doi.org/10.1002/sca.21249
  18. Ersahan S, Aydin C. Solubility and apical sealing characteristics of a new calcium silicate-based root canal sealer in comparison to calcium hydroxide-, methacrylate resin- and epoxy resin-based sealers. Acta Odontol Scand 2013;71:857-862. https://doi.org/10.3109/00016357.2012.734410
  19. Pawar SS, Pujar MA, Makandar SD. Evaluation of the apical sealing ability of bioceramic sealer, AH plus & epiphany: an in vitro study. J Conserv Dent 2014;17:579-582. https://doi.org/10.4103/0972-0707.144609
  20. Vertucci FJ. Root canal anatomy of the human permanent teeth. Oral Surg Oral Med Oral Pathol 1984;58:589-599. https://doi.org/10.1016/0030-4220(84)90085-9
  21. Torabinejad M, Ung B, Kettering JD. In vitro bacterial penetration of coronally unsealed endodontically treated teeth. J Endod 1990;16:566-569. https://doi.org/10.1016/S0099-2399(07)80198-1
  22. Chailertvanitkul P, Saunders WP, MacKenzie D, Weetman DA. An in vitro study of the coronal leakage of two root canal sealers using an obligate anaerobe microbial marker. Int Endod J 1996;29:249-255. https://doi.org/10.1111/j.1365-2591.1996.tb01377.x
  23. Tay FR, Loushine RJ, Lambrechts P, Weller RN, Pashley DH. Geometric factors affecting dentin bonding in root canals: a theoretical modeling approach. J Endod 2005;31:584-589. https://doi.org/10.1097/01.don.0000168891.23486.de
  24. Kontakiotis EG, Wu MK, Wesselink PR. Effect of sealer thickness on long-term sealing ability: a 2-year follow-up study. Int Endod J 1997;30:307-312. https://doi.org/10.1111/j.1365-2591.1997.tb00715.x
  25. Candeiro GT, Correia FC, Duarte MA, Ribeiro-Siqueira DC, Gavini G. Evaluation of radiopacity, pH, release of calcium ions, and flow of a bioceramic root canal sealer. J Endod 2012;38:842-845. https://doi.org/10.1016/j.joen.2012.02.029
  26. Yucel AC, Guler E, Guler AU, Ertas E. Bacterial penetration after obturation with four different root canal sealers. J Endod 2006;32:890-893. https://doi.org/10.1016/j.joen.2006.04.002
  27. Zmener O, Spielberg C, Lamberghini F, Rucci M. Sealing properties of a new epoxy resin-based root-canal sealer. Int Endod J 1997;30:332-334. https://doi.org/10.1111/j.1365-2591.1997.tb00719.x
  28. Eldeniz AU, Orstavik D. A laboratory assessment of coronal bacterial leakage in root canals filled with new and conventional sealers. Int Endod J 2009;42:303-312. https://doi.org/10.1111/j.1365-2591.2008.01509.x
  29. Loushine BA, Bryan TE, Looney SW, Gillen BM, Loushine RJ, Weller RN, Pashley DH, Tay FR. Setting properties and cytotoxicity evaluation of a premixed bioceramic root canal sealer. J Endod 2011;37:673-677. https://doi.org/10.1016/j.joen.2011.01.003
  30. De-Deus G. Research that matters - root canal filling and leakage studies. Int Endod J 2012;45:1063-1064. https://doi.org/10.1111/j.1365-2591.2012.02104.x
  31. Brosco VH, Bernardineli N, Torres SA, Consolaro A, Bramante CM, de Moraes IG, Ordinola-Zapata R, Garcia RB. Bacterial leakage in obturated root canals-part 2: a comparative histologic and microbiologic analyses. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;109:788-794. https://doi.org/10.1016/j.tripleo.2009.11.036
  32. Hammad M, Qualtrough A, Silikas N. Evaluation of root canal obturation: a three-dimensional in vitro study. J Endod 2009;35:541-544. https://doi.org/10.1016/j.joen.2008.12.021
  33. Epley SR, Fleischman J, Hartwell G, Cicalese C. Completeness of root canal obturations: epiphany techniques versus gutta-percha techniques. J Endod 2006;32:541-544. https://doi.org/10.1016/j.joen.2005.10.059
  34. James BL, Brown CE, Legan JJ, Moore BK, Vail MM. An in vitro evaluation of the contents of root canals obturated with gutta percha and AH-26 sealer or Resilon and Epiphany sealer. J Endod 2007;33:1359-1363. https://doi.org/10.1016/j.joen.2007.07.021
  35. Sevimay S, Dalat D. Evaluation of penetration and adaptation of three different sealers: a SEM study. J Oral Rehabil 2003;30:951-955. https://doi.org/10.1046/j.1365-2842.2003.01084.x
  36. Silva RV, Silveira FF, Horta MC, Duarte MA, Cavenago BC, Morais IG, Nunes E. Filling effectiveness and dentinal penetration of endodontic sealers: a stereo and confocal laser scanning microscopy study. Braz Dent J 2015;26:541-546. https://doi.org/10.1590/0103-6440201300138
  37. Zhejun W. Bioceramic materials in endodontics. Endod Topics 2015;32:86-96. https://doi.org/10.1111/etp.12074
  38. Ricucci D, Grondahl K, Bergenholtz G. Periapical status of root-filled teeth exposed to the oral environment by loss of restoration or caries. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2000;90:354-359. https://doi.org/10.1067/moe.2000.108802

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