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Effects of Daegangwhal-Tang Hot Aqueous Extract on Anti-inflammation and Anti-oxidation in RAW 264.7 Macrophage

  • Lee, Jae Sung (Department of Acupuncture & Moxibustion Medicine, Semyung University Chungju Hospital of Korean Medicine) ;
  • Jo, Na Young (Department of Acupuncture & Moxibustion Medicine, Semyung University Jecheon Hospital of Korean Medicine) ;
  • Roh, Jeong Du (Department of Acupuncture & Moxibustion Medicine, Semyung University Jecheon Hospital of Korean Medicine) ;
  • Lee, Cham Kyul (Department of Acupuncture & Moxibustion Medicine, Semyung University Chungju Hospital of Korean Medicine) ;
  • Lee, Eun Yong (Department of Acupuncture & Moxibustion Medicine, Semyung University Chungju Hospital of Korean Medicine)
  • 투고 : 2018.05.21
  • 심사 : 2018.08.03
  • 발행 : 2018.08.30

초록

Background: The objective of this study was to determine the effects of Daegangwhal-Tang (DGHT) hot aqueous extract on production of inflammatory mediators and antioxidants in RAW 264.7 macrophage. Methods: DGHT was extracted with water, filtered, concentrated and freeze-dried to perform. Cytotoxicity of DGHT extract was performed by MTT assay. Activated macrophages were treated with varying concentrations of DGHT extract (10, 50, 100 and $200{\mu}g/mL$), and nitric oxide (NO) and prostaglandin E2 ($PGE_2$) concentrations were measured to detect anti-oxidative effects. Interleukin-6 (IL-6), interleukin-1 beta ($IL-1{\beta}$) and tumor necrosis factor-alpha($TNF-{\alpha}$) concentrations were also measured to detect inflammatory responses to DGHT Results: Cytotoxicity of DGHT extract at concentrations of 10, 50, 100 and $200{\mu}g/mL$ were not observed. NO production was significantly decreased in the DGHT hot aqueous extract $200{\mu}g/mL$ concentration group. $PGE_2$, IL-6, $IL-1{\beta}$ and $TNF-{\alpha}$ production was significantly decreased in the DGHT hot aqueous extract 100 and $200{\mu}g/mL$ concentration groups. DGHT hot aqueous extract appeared to have DPPH free radical scavenging capability at all of concentrations, but did not exceed 50%. Conclusion: These results suggest that DGHT hot aqueous extract has concentration-dependent anti-inflammatory and anti-oxidative effect.

키워드

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