The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
권호 표지
DOI QR코드

DOI QR Code

The influences of Phaseolus radiatus L.'s Ethanol Extracts and Fractions on Skin Whitening and Anti-inflammatory Effects

녹두(綠豆) 에탄올 추출물 및 분획물이 피부의 미백, 항염증 효과에 미치는 영향

  • Lee, Yu-Lim (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Choi, Jung-Hwa (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Park, Soo-Yeon (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Jeong, Min-Yeong (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Lee, Ho-Chan (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University) ;
  • Song, Jie-Hye (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Dongshin University)
  • 이유림 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 최정화 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 박수연 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 정민영 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 이호찬 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 송지혜 (동신대학교 한의과대학 안이비인후피부과학교실)
  • Received : 2018.07.07
  • Accepted : 2018.08.11
  • Published : 2018.08.25
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : This study is designed to clarify whitening, anti-inflammatory effect of fractions extracted from the mixture of Phaseolus radiatus L. and ethanol. Methods : In this experiment, we were intended to reveal whitening, anti-inflammatory effect of fractions extracted from the mixture of Phaseolus radiatus L. and ethanol. The whitening activity was confirmed by UV blocking activity, tyrosinase inhibiting activity, and melanin formation inhibiting activity. Anti-inflammatory activity is confirmed by measurement of cytotoxicity level by MTT assay and measurement of Cytokine expression, which is the main mediator of inflammation reaction. Results : As a results, overall activity was high in the ethyl acetate fraction. Tyrosinase inhibitory activity was less than 20% at all concentrations, but the activity to inhibit melanin self-production was higher than that of ethyl acetate fraction at $32.19{\pm}2.79%$ at $100{\mu}g/m{\ell}$. And ethyl acetate fraction had a relatively high UV blocking activity. In the anti-inflammatory test, the concentration-dependent activity was shown, and the chloroform and ethyl acetate fractions showed significant NO production inhibitory activity. Cytokine expression was superior to that of the final stage of B cell differentiation, and cell viability was over 80% except for the chloroform fraction at the concentration of $200{\mu}g/m{\ell}$. Conclusions : The results of this experiment confirmed the whitening effect and anti-inflammatory effect of Phaseolus radiatus L.'s extracts and fractions and report the possibility of application as external medicine.

Keywords

References

  1. Kang JH, Song CH, Ock SM, Park HJ. Particulate Matter and Skin. Korean J Fam Pract. 2014;4:116-21.
  2. Yoon YM, Bae SH, An SK, Choe YB, Ahn KJ, An IS. Effects of Ultraviolet Radiation on the Skin and Skin Cell Signaling Pathways. Kor J Aesthet Cosmetol. 2013;11(3):417-26.
  3. Oh MH, Park HJ, Lee SH, Park SY. The effect of Well-being Lifestyle on Perceived Value and Purchase Intention of Naturalistic Cosmetics. Asian Journal of Beauty and Cosmetology. 2010;8(3):175-90.
  4. Son DS. Depigmentation activity of Kakamseosiokyong-san. 2002.
  5. Nam OY. Study on Effect of Seosiokyongsan in the Signaling Pathway of Melanogenesis. 1999.
  6. Heo J. Dong-ui-bo-gam, yoe-hyeong-pyeon.
  7. Committe of Oriental Medicine book. Herbal medicine. Seoul:Yeong-lim-sa. 2004;262-3.
  8. Wi HR, Choi MJ, Choi SL, Kim AJ, Lee MS. Effects of Vitexin from Mung Bean on 3T3-L1 Adipocyte Differentiation and Regulation According to Adipocytokine Secretion. J Korean Soc Food Sci Nutr. 2012;41(8):1079-85. https://doi.org/10.3746/jkfn.2012.41.8.1079
  9. Oh HS, Kim JH, Lee MH. Isoflavone Contents, Antioxidative and Fibrinolytic Activities of Red Bean and Mung Bean. Korean J Soc Food Cookery Sci. 2003;19(3):263-70.
  10. Im JY, Kim SJ. Anti-cancer and Anti-inflammatory Effects of Mung Bean and Soybean Extracts. Korean J Food Sci Technol. 2010;42(6):755-61.
  11. Seo IA. Studies on cosmeceutical of Phaseolus radiatus. 2017.
  12. Lee NR. Anti-irritation Properties of Germinated Mung Bean(Phaseolus radiatus L..) Extract as a Cosmetic Ingredient. 2017.
  13. Jung SW, Lee NK, Kim SJ, Han DS. Screening of tyrosinase inhibitor from plants. Korean J Food Sci Technol. 1995;27:891-96.
  14. Hosoi J, Abe E, Suda T, Kuroki T. Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res. 1985;45:1474-8.
  15. Ding A. H. Nathan, C. F. and Stuehr, D. J. Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages. Comparison of activating cytokines and evidence for independent production. J. Immunol. 1988;141:2407-12.
  16. Shin KM, Park YM, Kim IT, Hong SP, Hong JP, Lee KT. In vitro antiinflammatory activity of amygdalin in murine macrophage Raw 264.7 cells. Korean Journal of Pharmacognosy. 2003;34:223-7.
  17. Bae YJ, Lee YH. A Study of the correlation between realization and image for cosmetics-Focusing on natural cosmetics. Journal of Beauty art management. 2013:7(3):273-82.
  18. Seo YL. A Study on the Development of Functional Beverage Using Mungbean. 2015.
  19. Jin YM. Tyrosinase Inhibitory Flavone and Flavonol from Stachys sieboldii Miq. 2007.
  20. Chun HJ, Choi EY, Yoon SC, Nam HW, Baek SH, Woo WH. Inhibitory Effects of Ethanol Extract of Atractylodis Rhizoma alba on Melanin Biosynthesis. YAKHAK HOEJI. 2001;45(3):269-75.
  21. Kim MJ, Ryu MJ. Inhibition of Melanogenesis and Anti-UV properties 'Reynoutria elliptica. Kor.J.Aesthet.Cosmetol. 2012;10(4):961-68.
  22. Kim J, Kwon IH, Im HJ, Im KS, Hwang CY. Inhibitory Effect on Melanogenesis of Radix Glycyrrhizae Water Extract. Korean J Oriental Physiology&Pathology. 2002;16(6):1230-35.
  23. Development of functional dairy products containing novel anti-inflammation components and active saponins from mung bean.MAFRA. 2012.
  24. Choi JE, Park SJ, Kim CW, Lee HS, Mun SY, Hwang YW, et al. Characterization of Anti-inflammation Compounds Isolated from Natural Products for Skin Health Improvement.WISET Junior Science & Technology Research Reports Vol1. 2014.
  25. Kwon ES, Kim H, Kwon H. Inhibitory effects on the enzymes involved in the inflammation by the ethanol extracts of plant foodstuffs. Korean J Food Sci Technol. 2007;39:348-52.