Clinical and Experimental Pediatrics

The Korean Pediatric Society (대한소아청소년과학회)
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Long-term prenatal stress increases susceptibility of N-methyl-D-aspartic acid-induced spasms in infant rats

  • Kwon, Hyeok Hee (Department of Medical Science, College of Medicine, Chungnam National University) ;
  • Lee, Taekwan (Department of Pediatrics, Chungnam National University Hospital, Chungnam National University School of Medicine) ;
  • Hong, Jinpyo (Department of Anatomy, College of Medicine, Chungnam National University) ;
  • Kim, Dong Woon (Department of Medical Science, College of Medicine, Chungnam National University) ;
  • Kang, Joon Won (Department of Medical Science, College of Medicine, Chungnam National University)
  • Received : 2017.07.10
  • Accepted : 2017.11.09
  • Published : 2018.05.15
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Abstract

Purpose: Infantile spasms, also known as West syndrome, is an age-specific epileptic seizure. Most patients with this condition also exhibit delayed development. This study aimed to determine the effect of long-term prenatal stress on susceptibility to infantile spasms. Methods: We subjected pregnant rats to acute or chronic immobilization stress. Resulting offspring received N-methyl-D-aspartic acid (15 mg/kg, intraperitoneally) on postnatal day 15, and their behaviors were observed 75 minutes after injection. The expression of KCC2 and GAD67 was also determined using immunohistochemistry. Results: Exposure to long-term prenatal stress increased the frequency of spasms and decreased the latency to onset of spasms compared with offspring exposed to short-term prenatal stress. Expression of KCC2 and GAD67 also decreased in the group exposed to long-term prenatal stress compared with the group exposed to short-term prenatal stress. Conclusion: Our study suggests that exposure to long-term prenatal stress results in increased susceptibility to seizures.

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References

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