Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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The Assessment of Photochemical Index of Nursery Seedlings of Cucumber and Tomato under Drought Stress

건조스트레스에 의한 오이와 토마토 공정육묘의 광화학적 지표 해석

  • Ham, Hyun Don (Institute of Ecological Phytochemistry and Department of Plant Life and Environmental Science, Hankyong National University) ;
  • Kim, Tae Seong (Institute of Ecological Phytochemistry and Department of Plant Life and Environmental Science, Hankyong National University) ;
  • Lee, Mi Hyun (Institute of Ecological Phytochemistry and Department of Plant Life and Environmental Science, Hankyong National University) ;
  • Park, Ki Bae (Institute of Ecological Phytochemistry and Department of Plant Life and Environmental Science, Hankyong National University) ;
  • An, Jae-Ho (Department of Civil, Safety & Environmental Engineering, Hankyong National University) ;
  • Kang, Dong Hyeon (Department of Agricultural Engineering, National Academy of Agricultural Science, RDA) ;
  • Kim, Tae Wan (Institute of Ecological Phytochemistry and Department of Plant Life and Environmental Science, Hankyong National University)
  • 함현돈 (국립한경대학교 식물생명환경과학과 식물생태화학연구소) ;
  • 김태성 (국립한경대학교 식물생명환경과학과 식물생태화학연구소) ;
  • 이미현 (국립한경대학교 식물생명환경과학과 식물생태화학연구소) ;
  • 박기배 (국립한경대학교 식물생명환경과학과 식물생태화학연구소) ;
  • 안재호 (국립한경대학교 토목안전환경공학과) ;
  • 강동현 (국립농업과학원 농업공학부) ;
  • 김태완 (국립한경대학교 식물생명환경과학과 식물생태화학연구소)
  • Received : 2018.10.22
  • Accepted : 2018.11.08
  • Published : 2018.12.31
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Abstract

The purpose of this study is to analyze photochemical activity of nursery seedlings under drought stress, using chlorophyll fluorescence reaction analysis. Young nursery seedlings of tomato (Lycopersicon esculentum Mill.) and cucumber (Cucumis sativa L.), were grown under drought stress for 8 days. Analysis of chlorophyll fluorescence reaction (OJIP) and parameters, were performed to evaluate photochemical fluctuation in nursery seedlings under drought stress. Chlorophyll fluorescence reaction analysis showed maximal recorded fluorescence (P) decreased from the 5 day after treatment in tomato seedlings, while an amount of chlorophyll fluorescence increased at the J-I step. Thus, physiological activity was reduced. In cucumber seedlings, maximal recorded fluorescence (P) and maximal variable fluorescence ($F_V$) lowered from the 4 day after treatment, and chlorophyll fluorescence intensity of J-I step increased. Chlorophyll fluorescence parameter analysis showed electron transfer efficiency of PSII and PSI were significantly inhibited with decreasing $ET2_O/RC$ and $RE1_O/RC$ from the 5 day after treatment, in tomato seedlings and from the 4 day after treatment, in cucumber seedlings. $ET2_O/RC$ and $PI_{ABS}$ significantly changed. In conclusion, 6 indices such as $F_V/F_M$, $DI_O/RC$, $ET2_O/RC$, $RE1_O/RC$, $PI_{ABS}$ and $PI_{TOTAL}ABS$ were selected for determining drought stress in nursery seedlings. Drought stress factor index (DFI) was used to evaluate whether the crop was healthy or not, under drought stress. Cucumber seedlings were less resistant to drought stress than tomato seedlings, in the process of drought stress.

본 연구는 엽록소형광반응 분석을 이용하여 건조스트레스에 의한 공정육묘의 광화학적 활력을 분석하였다. 토마토와 오이 공정육묘를 8일 동안 건조스트레스 처리를 하였다. 엽록소형광반응(OJIP)과 매개변수 분석을 통해 건조스트레스로 인한 작물의 광화학적 변동을 평가하였다. 엽록소 형광반응(OJIP) 분석 결과, 토마토는 처리 후 5일부터 최대 형광량(P)이 감소한 반면 J-I 단계에서는 엽록소 형광량이 증가하였다. 따라서 생리적 활력이 감소한 것을 알 수 있었다. 오이의 경우 처리 후 4일부터 최대 형광(P) 및 변동 형광량($F_V$)이 낮아지고 J-I 단계의 엽록소 형광 수치가 증가하였다. 엽록소 형광 매개변수 분석한 결과 토마토는 처리 후 5일부터 특히 $ET2_O/RC$와 $RE1_O/RC$가 감소하면서 광계II와 광계I의 전자전달효율이 유의적으로 낮아진 것으로 보인 반면 오이는 처리 후 4일부터 $ET2_O/RC$와 $PI_{ABS}$가 상당히 변화하였다. 결론적으로 $F_V/F_M$, $DI_O/RC$, $ET2_O/RC$, $RE1_O/RC$, $PI_{ABS}$, $PI_{TOTAL}ABS$ 6개의 지표가 공정육묘의 건조스트레스를 판단하는 지표로 선정되었다. 건조스트레스지수(DFI)를 통해 건조스트레스로 인한 작물별 건전성 평가를 하였고 오이의 경우 토마토에 비해 건조 저항성이 낮은 것으로 판단되었다.

Keywords

References

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