Restorative Dentistry and Endodontics

  • 제43권4호
  • /
  • Pages.46.1-46.10
  • /
  • 2018
  • /
  • 2234-7658(pISSN)
  • /
  • 2234-7666(eISSN)
대한치과보존학회 (The Korean Academy of Conservative Dentistry)
권호 표지
DOI QR코드

DOI QR Code

Improved dentin disinfection by combining different-geometry rotary nickel-titanium files in preparing root canals

  • Bedier, Marwa M. (Department of Endodontics, Faculty of Dentistry, Cairo University) ;
  • Hashem, Ahmed Abdel Rahman (Department of Endodontics, Faculty of Dentistry, Ain Shams University) ;
  • Hassan, Yosra M. (Department of Clinical Pathology, Cairo University)
  • 투고 : 2018.06.19
  • 심사 : 2018.09.11
  • 발행 : 2018.11.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives: This study was to evaluate the antibacterial effect of different instrumentation and irrigation techniques using confocal laser scanning microscopy (CLSM) after root canal inoculation with Enterococcus faecalis (E. faecalis). Materials and Methods: Mesiobuccal and mesiolingual canals of extracted mandibular molars were apically enlarged up to a size 25 hand K-file, then autoclaved and inoculated with E. faecalis. The samples were randomly divided into 4 main groups according to the system of instrumentation and irrigation: an XP-endo Shaper (XPS) combined with conventional irrigation (XPS/C) or an XP-endo Finisher (XPF) (XPS/XPF), and iRaCe combined with conventional irrigation (iRaCe/C) or combined with an XPF (iRaCe/XPF). A middle-third sample was taken from each group, and then the bacterial reduction was evaluated using CLSM at a depth of $50{\mu}m$ inside the dentinal tubules. The ratio of red fluorescence (dead cells) to green-and-red fluorescence (live and dead cells) represented the percentage of bacterial reduction. The data were then statistically analyzed using the Kruskal-Wallis test for comparisons across the groups and the Dunn test was used for pairwise comparisons. Results: The instrumentation and irrigation techniques had a significant effect on bacterial reduction (p < 0.05). The iRaCe/XPF group showed the strongest effect, followed by the XPS/XPF and XPS/C group, while the iRaCe/C group had the weakest effect. Conclusions: Combining iRaCe with XPF improved its bacterial reduction effect, while combining XPS with XPF did not yield a significant improvement in its ability to reduce bacteria at a depth of $50{\mu}m$ in the dentinal tubules.

키워드

참고문헌

  1. Sjogren U, Figdor D, Persson S, Sundqvist G. Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. Int Endod J 1997;30:297-306. https://doi.org/10.1111/j.1365-2591.1997.tb00714.x
  2. Siqueira JF Jr, Lima KC, Magalhaes FA, Lopes HP, de Uzeda M. Mechanical reduction of the bacterial population in the root canal by three instrumentation techniques. J Endod 1999;25:332-335. https://doi.org/10.1016/S0099-2399(06)81166-0
  3. Schafer E, Zapke K. A comparative scanning electron microscopic investigation of the efficacy of manual and automated instrumentation of root canals. J Endod 2000;26:660-664. https://doi.org/10.1097/00004770-200011000-00007
  4. Paque F, Ganahl D, Peters OA. Effects of root canal preparation on apical geometry assessed by micro-computed tomography. J Endod 2009;35:1056-1059. https://doi.org/10.1016/j.joen.2009.04.020
  5. Paque F, Balmer M, Attin T, Peters OA. Preparation of oval-shaped root canals in mandibular molars using nickel-titanium rotary instruments: a micro-computed tomography study. J Endod 2010;36:703-707. https://doi.org/10.1016/j.joen.2009.12.020
  6. Sundqvist G, Figdor D, Persson S, Sjogren U. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998;85:86-93. https://doi.org/10.1016/S1079-2104(98)90404-8
  7. Pinheiro ET, Gomes BP, Ferraz CC, Sousa EL, Teixeira FB, Souza-Filho FJ. Microorganisms from canals of root-filled teeth with periapical lesions. Int Endod J 2003;36:1-11. https://doi.org/10.1046/j.1365-2591.2003.00603.x
  8. Orstavik D, Haapasalo M. Disinfection by endodontic irrigants and dressings of experimentally infected dentinal tubules. Endod Dent Traumatol 1990;6:142-149. https://doi.org/10.1111/j.1600-9657.1990.tb00409.x
  9. Peters OA, Arias A, Paque F. A micro-computed tomographic assessment of root canal preparation with a novel instrument, TRUShape, in mesial roots of mandibular molars. J Endod 2015;41:1545-1550. https://doi.org/10.1016/j.joen.2015.06.007
  10. Versiani MA, Pecora JD, de Sousa-Neto MD. Flat-oval root canal preparation with self-adjusting file instrument: a micro-computed tomography study. J Endod 2011;37:1002-1007. https://doi.org/10.1016/j.joen.2011.03.017
  11. Bayram HM, Bayram E, Ocak M, Uygun AD, Celik HH. Effect of ProTaper Gold, self-adjusting File, and XP-endo Shaper instruments on dentinal microcrack formation: a micro-computed tomographic study. J Endod 2017;43:1166-1169. https://doi.org/10.1016/j.joen.2017.02.005
  12. Elnaghy AM, Elsaka SE. Torsional resistance of XP-endo Shaper at body temperature compared with several nickel-titanium rotary instruments. Int Endod J 2018;51:572-576. https://doi.org/10.1111/iej.12815
  13. FKG Dentaire SA. The XP-endo Finisher file brochure [Internet]. La Chaux-de-Fonds: FKG Dentaire SA; c2014 [cited 2018 Aug 5]. Available from: https://www.fkg.ch/products/endodontics/canal-shaping-and-cleaning/xp-endo-finisher. (updated 2018 Oct 29).
  14. Silva EJ, Vieira VT, Belladonna FG, Zuolo AS, Antunes HD, Cavalcante DM, Elias CN, De-Deus G. Cyclic and torsional fatigue resistance of XP-endo Shaper and TRUShape instruments. J Endod 2018;44:168-172. https://doi.org/10.1016/j.joen.2017.08.033
  15. Elnaghy A, Elsaka S. Cyclic fatigue resistance of XP-endo Shaper compared with different nickel-titanium alloy instruments. Clin Oral Investig 2018;22:1433-1437. https://doi.org/10.1007/s00784-017-2245-5
  16. Trope M, Debelian G. XP-3D $Finisher^{TM}$ file-the next step in restorative endodontics. Endod Pract US 2015;8:22-24.
  17. Bao P, Shen Y, Lin J, Haapasalo M. In vitro efficacy of XP-endo Finisher with 2 different protocols on biofilm removal from apical root canals. J Endod 2017;43:321-325. https://doi.org/10.1016/j.joen.2016.09.021
  18. Leoni GB, Versiani MA, Silva-Sousa YT, Bruniera JF, Pecora JD, Sousa-Neto MD. Ex vivo evaluation of four final irrigation protocols on the removal of hard-tissue debris from the mesial root canal system of mandibular first molars. Int Endod J 2017;50:398-406. https://doi.org/10.1111/iej.12630
  19. Ozdemir HO, Buzoglu HD, Calt S, Stabholz A, Steinberg D. Effect of ethylenediaminetetraacetic acid and sodium hypochlorite irrigation on Enterococcus faecalis biofilm colonization in young and old human root canal dentin: in vitro study. J Endod 2010;36:842-846. https://doi.org/10.1016/j.joen.2010.01.008
  20. Wong DT, Cheung GS. Extension of bactericidal effect of sodium hypochlorite into dentinal tubules. J Endod 2014;40:825-829. https://doi.org/10.1016/j.joen.2013.09.045
  21. Shen Y, Qian W, Chung C, Olsen I, Haapasalo M. Evaluation of the effect of two chlorhexidine preparations on biofilm bacteria in vitro: a three-dimensional quantitative analysis. J Endod 2009;35:981-985. https://doi.org/10.1016/j.joen.2009.04.030
  22. Ordinola-Zapata R, Bramante CM, de Moraes IG, Bernardineli N, Porto CV, Campanelli AP, Garcia RB, Duarte MH. The use of confocal laser scanning microscopy for the study of dentin infection. In: Mendez-Vilas A, Diaz J, editors. Microscopy: science, technology, applications and education. Badajoz: Formatex; 2010. p.583-589.
  23. Silva EJ, Belladonna FG, Zuolo AS, Rodrigues E, Ehrhardt IC, Souza EM, De-Deus G. Effectiveness of XP-endo Finisher and XP-endo Finisher R in removing root filling remnants: a micro-CT study. Int Endod J 2018;51:86-91. https://doi.org/10.1111/iej.12788
  24. Azim AA, Aksel H, Zhuang T, Mashtare T, Babu JP, Huang GT. Efficacy of 4 irrigation protocols in killing bacteria colonized in dentinal tubules examined by a novel confocal laser scanning microscope analysis. J Endod 2016;42:928-934. https://doi.org/10.1016/j.joen.2016.03.009
  25. Schneider SW. A comparison of canal preparations in straight and curved root canals. Oral Surg Oral Med Oral Pathol 1971;32:271-275. https://doi.org/10.1016/0030-4220(71)90230-1
  26. George S, Kishen A, Song KP. The role of environmental changes on monospecies biofilm formation on root canal wall by Enterococcus faecalis. J Endod 2005;31:867-872. https://doi.org/10.1097/01.don.0000164855.98346.fc
  27. Siqueira JF Jr. Endodontic infections: concepts, paradigms, and perspectives. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002;94:281-293. https://doi.org/10.1067/moe.2002.126163
  28. Nakamura VC, Candeiro GT, Cai S, Gavini G. Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals. Braz Oral Res 2015;29:1-7.
  29. Stewart PS. New ways to stop biofilm infections. Lancet 2003;361:97.
  30. Haapasalo M, Orstavik D. In vitro infection and disinfection of dentinal tubules. J Dent Res 1987;66:1375-1379. https://doi.org/10.1177/00220345870660081801
  31. Nair PN, Henry S, Cano V, Vera J. Microbial status of apical root canal system of human mandibular first molars with primary apical periodontitis after "one-visit" endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005;99:231-252. https://doi.org/10.1016/j.tripleo.2004.10.005
  32. Spratt DA, Pratten J, Wilson M, Gulabivala K. An in vitro evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates. Int Endod J 2001;34:300-307. https://doi.org/10.1046/j.1365-2591.2001.00392.x
  33. Ma J, Wang Z, Shen Y, Haapasalo M. A new noninvasive model to study the effectiveness of dentin disinfection by using confocal laser scanning microscopy. J Endod 2011;37:1380-1385. https://doi.org/10.1016/j.joen.2011.06.018
  34. Love RM. Regional variation in root dentinal tubule infection by Streptococcus gordonii. J Endod 1996;22:290-293. https://doi.org/10.1016/S0099-2399(96)80261-5
  35. Carrigan PJ, Morse DR, Furst ML, Sinai IH. A scanning electron microscopic evaluation of human dentinal tubules according to age and location. J Endod 1984;10:359-363. https://doi.org/10.1016/S0099-2399(84)80155-7
  36. Paque F, Musch U, Hulsmann M. Comparison of root canal preparation using RaCe and ProTaper rotary Ni-Ti instruments. Int Endod J 2005;38:8-16. https://doi.org/10.1111/j.1365-2591.2004.00889.x
  37. Azim AA, Piasecki L, da Silva Neto UX, Cruz AT, Azim KA. XP Shaper, a novel adaptive core rotary instrument: micro-computed tomographic analysis of its shaping abilities. J Endod 2017;43:1532-1538. https://doi.org/10.1016/j.joen.2017.04.022
  38. Lacerda MF, Marceliano-Alves MF, Perez AR, Provenzano JC, Neves MA, Pires FR, Goncalves LS, Rocas IN, Siqueira JF Jr. Cleaning and shaping oval canals with 3 instrumentation systems: a correlative microcomputed tomographic and histoligical study. J Endod 2017;43:1878-1884. https://doi.org/10.1016/j.joen.2017.06.032
  39. Zupanc J, Vahdat-Pajouh N, Schafer E. New thermomechanically treated NiTi alloys - a review. Int Endod J 2018;51:1088-1103. https://doi.org/10.1111/iej.12924
  40. FKG Dentaire SA. FKG XP-endo Shaper brochure [Internet]. La Chaux-de-Fonds: FKG Dentaire SA; c2014 [cited 2018 Aug 5]. Available from: https://www.fkg.ch/sites/default/files/201704_fkg_xp_endo_shaper_brochure_v4_fr_web.pdf. (updated 2018 Oct 29).
  41. Alves FR, Andrade-Junior CV, Marceliano-Alves MF, Perez AR, Rocas IN, Versiani MA, Sousa-Neto MD, Provenzano JC, Siqueira JF Jr. Adjunctive steps for disinfection of the mandibular molar root canal system: a correlative bacteriologic, micro-computed tomography, and cryopulverization approach. J Endod 2016;42:1667-1672. https://doi.org/10.1016/j.joen.2016.08.003
  42. Elnaghy AM, Mandorah A, Elsaka SE. Effectiveness of XP-endo Finisher, EndoActivator, and File agitation on debris and smear layer removal in curved root canals: a comparative study. Odontology 2017;105:178-183. https://doi.org/10.1007/s10266-016-0251-8
  43. Sanabria-Liviac D, Moldauer BI, Garcia-Godoy F, Antonio-Campos A, Casaretto M, Torres-Navarro J, Scalercio JM. Comparison of the XP-Endo Finisher file system and passive ultrasonic irrigation (PUI) on smear layer removal after root canal instrumentation. J Dent Oral Health 2017;4:1-7.

피인용 문헌

  1. Irrigation in Endodontics: a Review vol.6, pp.4, 2018, https://doi.org/10.1007/s40496-019-00241-6
  2. The Effects of Different Endodontic Access Cavity Design and Using XP-endo Finisher on the Reduction of Enterococcus faecalis in the Root Canal System vol.46, pp.3, 2020, https://doi.org/10.1016/j.joen.2019.11.011
  3. The effectiveness of the supplementary use of the XP-endo Finisher on bacteria content reduction: a systematic review and meta-analysis vol.46, pp.3, 2021, https://doi.org/10.5395/rde.2021.46.e37
  4. Combination of a new ultrasonic tip with rotary systems for the preparation of flattened root canals vol.46, pp.4, 2018, https://doi.org/10.5395/rde.2021.46.e56
  5. Complete Obturation-Cold Lateral Condensation vs. Thermoplastic Techniques: A Systematic Review of Micro-CT Studies vol.14, pp.14, 2021, https://doi.org/10.3390/ma14144013