생명과학회지 (Journal of Life Science)

  • 제28권7호
  • /
  • Pages.841-848
  • /
  • 2018
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

초석잠 및 택란 추출물의 플라보노이드 함량 및 항산화 활성 비교

Comparison of Flavonoid Content and Antioxidant Effect of Extracts from Stachys sieboldii Miq. and Lycopus lucidus Turcz

  • 이정우 (한국해양대학교 해양생명과학부) ;
  • 임선영 (한국해양대학교 해양생명과학부)
  • Lee, Jung Woo (Division of Marine Bioscience, Korea Maritime and Ocean University) ;
  • Lim, Sun Young (Division of Marine Bioscience, Korea Maritime and Ocean University)
  • 투고 : 2018.03.02
  • 심사 : 2018.04.19
  • 발행 : 2018.07.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

초석잠 acetone+methylene chloride (A+M) 및 methanol (MeOH) 추출물들의 총 플라보노이드 함량은 $57.05{\pm}1.08$ 및 $37.42{\pm}0.12mg/g$으로 A+M 추출물의 총 플라보노이드 함량이 높았다. 택란 A+M 및 MeOH 추출물들의 총 플라보노이드 함량은 $233.22{\pm}2.60$ 및 $46.31{\pm}0.54mg/g$으로 A+M 추출물의 총 플라보노이드 함량이 높았다 이에 따라 총 플라보노이드 함량은 A+M 추출물에서 높게 나타남을 알 수 있었다. 각 용매별 추출물들의 1,1-Diphenyl-2-picryhydrazyl (DPPH) 라디칼 소거활성을 각각 0.05, 0.1, 0.25 및 0.5 mg/ml의 농도로 대조군 [L-ascorbic acid, Butylated hydroxyl toluene (BHT)]과 비교하였다. 먼저 추출물들과 비교했을 때 택란 A+M 추출물은 다른 추출물과 비교했을 때 활성산소 소거능이 우수하였다. 이는 앞서 택란 A+M 추출물의 높은 총 플라보노이드 및 총 페놀 함량과 연관성이 있는 것으로 여겨진다. 2.2'-Azino-bis (3-ethylbenothiazoline-6-sulfonic acid) diammonium salt radical cation (ABTS+) 라디칼 소거활성에서도 택란 A+M추출물과 초석잠 A+M추출물은 합성 항산화제인 BHT와 비교하여 0.25 및 0.5 mg/ml 농도에서 BHT 경우, 93.5 및 95.3%이며, 초석잠 A+M추출물은 89.0 및 89.8%의 소거능을 나타내었으며 택란 A+M추출물은 각각90.2% 및 88.0%로 대조군과 유사한 값을 나타내었다. 초석잠 및 택란 A+M 및 MeOH 추출물을 0.05 및 0.025 mg/ml의 농도로 인체 섬유육종세포(HT-1080)에 처리하여 세포 내 활성 산소종을 측정한 결과 두 추출물들 모두 측정시간 120분이 지남에 따라 높은 세포 내 활성산소종 억제효과를 나타내었다. A+M 추출물의 경우 MeOH 추출물과 비교 하였을 때 세포 내 활성산소종을 상대적으로 크게 억제하였으며 특히 택란 A+M 추출물 0.05 mg/ml 농도에서는 대조군과 비교하여 높은 억제효과를 나타내었다. 따라서 본 연구 결과는 택란 A+M 추출물에 의한 항산화 효과가 우수하였고 이는 택란 초석잠 A+M 추출물의 높은 함량의 플라보노이드와도 연관성이 있음을 나타낸다.

The flavonoid content and antioxidant effects of extracts from Stachys sieboldii Miq. and Lycopus lucidus Turcz were compared. The flavonoid content of the acetone + methylene chloride (A+M) extract of L. lucidus Turcz was 233.2 mg/g, suggesting that the extract was greater than that of S. sieboldii Miq. In the DPPH assay and the A+M and methanol (MeOH) extracts from L. lucidus Turcz had greater scavenging effects than those of S. sieboldii Miq. (p<0.05). The A+M extract from L. lucidus Turcz (0.5 mg/ml concentration) had an 82% scavenging effect in the DPPH assay. In the ABTS assay, A+M extracts from both S. sieboldii Miq. and L. lucidus Turcz (0.5 mg/ml concentration) had scavenging effects of 90% and 88%, respectively (p<0.05), suggesting that both A+M extracts had greater scavenging effects than those of both MeOH extracts. In a 120 min ROS production assay, all tested extracts dose-dependently decreased the cellular ROS production that was induced by $H_2O_2$, as compared to those produced by exposure to the extract-free control. The A+M extracts from both S. sieboldii Miq. and L. lucidus Turcz had greater inhibitory effects on cellular ROS production than those of both MeOH extracts at all concentrations tested. Treatment with the A+M extracts from S. sieboldii Miq. and L. lucidus Turcz (0.25 mg/ml concentration) inhibited the cellular ROS production by 60% and 86%, respectively. These results suggest that the A+M extracts of Stachys sieboldii Miq. and L. lucidus Turcz inhibit cellular oxidation and may contain valuable bioactive compounds, such as flavonoids.

키워드

참고문헌

  1. Bae, J. H., Jang, J. R. and Lim, S. Y. 2014. Production of glutathione and reactive oxygen species in cell line HT-1080 treated with chub mackerel (Scomber japonicus) extracts. Philipp. Agric. Sci. 97, 180-184.
  2. Baek, H. S., Song, S. K., Na, Y. S. and Ryu, B. H. 2003. Antioxidant activities of Stachys sieboldii MIQ. stalks. Kor. J. Biotechnol. Bioeng. 18, 266-271.
  3. Baek, H. S., Na, Y. S., Kim, D. H., Lee, C. H., Ryu, B. H. and Song, S. K. 2004. Antioxidant Activities of Stachys sieboldii MIQ Roots. J. Life Sci. 14, 1-7. https://doi.org/10.5352/JLS.2004.14.1.001
  4. Chae, S. K., Kang, G. S., Ma, S. J., Bang, K. W., Oh, M. M. and Oh, S. H. 2002. Standard food analysis. Jigu publishing Co Paju, Korea p 381-382.
  5. Chen, H. H., Muranmoto, K., Yamauchi, F., Fujimoto, K. and Nokihara, K. 1995. Antioxidative properties of histidine-containing peptides designed from peptide fragment found in the digests of a soybean protein. J. Agric. Food Chem. 46, 49-53.
  6. Chung, M. J. and Lee, C. Y. 1993. Antioxidative characteristics of plant phenolic compounds. J. Life Sci. 3, 9-17.
  7. Donida, B., Jacques, C. E. D., Mescka, C. P., Rodrigues, D. G. B., Marcheti, D. P., Ribas, G., Gliugliani, R. and Vargas, C. R. 2017. Oxidative damage and redox in lysosomal storage disorders: Biochemical markers. Clin. Chim. Acta 466, 46-53. https://doi.org/10.1016/j.cca.2017.01.007
  8. Feng, K. F., Chen, W., Sun, L., Liu, J., Zhao, Y., Li, L., Wang, Y. and Zhang, W. 2015. Optimization extraction, preliminary characterization and antioxidant activity in vitro of polysaccharides from Stachys sieboldii Miq. Tubers. Carbohyd. Polym. 125, 45-52. https://doi.org/10.1016/j.carbpol.2015.02.026
  9. Heim, K. E., Tagliaferro, A. R. and Bobiya, D. J. 2002. Flavonoid antioxidants: Chemistry, metabolism, and structure-activity relationships. J. Nutr. Biochem. 13, 572-584. https://doi.org/10.1016/S0955-2863(02)00208-5
  10. Hetog, M. G. L., Hollman, P. C. H. and Van de Putter, B. 1993. Content of potentially anticccarcinogeic flavonoids of tea infusions, wines and fruit juice. J. Agric. Food Chem. 41, 1242-1246. https://doi.org/10.1021/jf00032a015
  11. Jeon, K. S. and Park, S. I. 2015. Antioxidative Properties of Chinese Artichoke (Stachys sieboldii Miq) added white bread. Kor. J. Culinary Res. 21, 120-132.
  12. Kehrer, J. P. and Klotz, L. O. 2015. Free radicals and related reactive species as mediators of tissue injury and disease: implications for health. Crit. Rev. Toxicol. 45, 765-798. https://doi.org/10.3109/10408444.2015.1074159
  13. Kim, D. Y. and Ghil, S. H. 2009. Effect of Lycopus lucidus Trucz on cell growth of human breast cancer cells, MCF-7. J. Exo. Biomed. Sci. 15, 147-152.
  14. Kim, E. J., Choi, J. Y., Yu, M. R., Kim, M. Y., Lee, S. H. and Lee, B. H. 2012. Total polyphenols, total flavonoid contents, and antioxidant activity of Korean natural and medicinal plants. Kor. J. Soc. Food Sci. Technol. 44, 337-342. https://doi.org/10.9721/KJFST.2012.44.3.337
  15. Kim, J. W., Moon, J. S. and Choe, T. B. 2014. Comparison of antioxidant activity of Kenaf extract and its flavonoids. Kor. J. Aesthet. Cosmetol. 12, 203-210.
  16. Kong, C. S., Um, Y. R., Lee, J. I., Kim, Y. A., Lee, J. S. and Seo, Y. W. 2008. Inhibition effect of extracts and its solvent fractions isolated from Limonium tetragonum on the growth of human cancer cells. Kor. J. Biotechnol. Bioeng. 23, 177-182.
  17. LeBel, C. P., Ischiropoulos, H. and Bondy, S. C.1992. Evaluation of the probe 2', 7'-Dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress. Chem. Res. Toxicol. 5, 227-231. https://doi.org/10.1021/tx00026a012
  18. Lee, J. E., Jin, S. Y. and Han, Y. S. 2014. Antioxidant activities and quality characteristics of tufu supplemented with Chinese artichoke powder. Kor. J. Food Nutr. 27, 10-21. https://doi.org/10.9799/ksfan.2014.27.1.010
  19. Lee, Y. J., Kang, D. G., Kim, J. S. and Lee, H. S. 2008. Lycopus lucidus inhibits high glucose-induced vascular inflammation in human umbilical vein endothelial. Vascul. Pharmacol. 48, 38-46. https://doi.org/10.1016/j.vph.2007.11.004
  20. Lin, C. R., Zuo, S. Y., Xiong, W. and Chen, G. Y. 2012. Antioxidation effects of Lycopus lucidus polysaccharides on aged mice induced D-galactose. Medicinal Plants 3, 53-55.
  21. Lu, Y. H., Huang, J. H., Li, Y., Ma, T., Sang, P., Wang, W. and Gao, C. 2015. Variation in nutritional compositions, antioxidant activity and microstructure of Ly root at different harvest times. Food Chem. 183, 91-100. https://doi.org/10.1016/j.foodchem.2015.03.033
  22. Na, B. R. and Lee, J. H. 2017. Antioxidative capacities Stachys sieboldii Miq. of and Ginseng powders and their effects on quality characteristics of cookies. J. Kor. Soc. Food Sci. Nutr. 46, 68-76 https://doi.org/10.3746/jkfn.2017.46.1.068
  23. Park, H. J., Jin, S., Oh, Y. N., Yun, S. G., Lee, J. Y., Kwon, H. J. and Kim, B. W. 2013. Induction of G1 arrest by methanol extract of Lycopus lucidus in human lung adenocarcinoma A549 cells. J. Life Sci. 23, 1109-1117. https://doi.org/10.5352/JLS.2013.23.9.1109
  24. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26, 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  25. Ryu, B. H. and Kim, S. O. 2004. Effects of methanol extract of Stachys sieboldii Miq. on acetylcholine esterase and monoamine oxidase in rat brain. Kor. J. Food Nutr. 17, 347-355.
  26. Slusarczyk, S., Hajnos, M., Skalicka-Wozniak, K. and Matkowski, A. 2009. Antioxidant activity of polyphenols from Lycopus lucidus Turz. Food Chem. 113, 134-138. https://doi.org/10.1016/j.foodchem.2008.07.037
  27. Sohn, H. Y., Ryu, H. Y., Jang, H. S., Park, Y. M. and Kim, S. Y. 2008. Evaluation of antimicrobial, antithrombin, and antioxidant activity of aerial part of Saxifraga stolonifera. Kor. J. Microbiol. Biotrechnol. 36, 195-200.
  28. Song, Y. J., Chang, J. P. and Yoo, J. H. 2016. Antioxidant activitites of water extracts from different parts of Lycopus lucidus Turcz. ex Benth. Kor. J. Herbaology 31, 21-28.
  29. Takeda, Y., Fujita, T., Satoh, T. and Kakegawa, H. 1985. On the glycosidic constituents of Stachys sieboldi MIQ. and their effects on hyaluronidase activity. Yakugaku Zasshi: J. Pharmaceutical Soc. Jp. 105, 955-959. https://doi.org/10.1248/yakushi1947.105.10_955
  30. Williams, R. J., Spencer, J. P. and Rice-Evans, C. 2004. Flavonoids: Antioxidants or signaling molecules? Free Radic. Biol. Med. 36, 838-849. https://doi.org/10.1016/j.freeradbiomed.2004.01.001
  31. Woo, E. R. and Piao, M. S. 2004. Antioxidative constituents from Lycopus lucidus. Arch. Pharmacol. Res. 27, 173-176. https://doi.org/10.1007/BF02980102
  32. Yang, M. R. 2012. The analysis of bioactive materials in Stachys siebolidii Miq. And its application on functional ready-to-eat food. PhD Dissertation. Gyengnam National University, Chagwon, Korea.
  33. Yang, M. O. 2017. Antioxidant properties of hot water extract of Lycopus lucidus Turcz Tubers. Kor. J. Community Living Sci. 28, 103-113. https://doi.org/10.7856/kjcls.2017.28.1.103