생명과학회지 (Journal of Life Science)

  • 제28권7호
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  • Pages.802-810
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  • 2018
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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바나나 껍질 에탄올 추출물이 멜라닌 합성에 미치는 영향

Positive Effect of Musa paradisiaca Peel Ethanolic Extract on Antioxidant Activity and Melanin Synthesis

  • 투고 : 2018.02.23
  • 심사 : 2018.04.24
  • 발행 : 2018.07.30
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초록

노화란 나이가 들면서 생기는 몸의 변화로 검은 머리가 흰 머리로 변하는 백발화, 과산화 지질과 단백질로 구성되어 생성되는 검버섯, 주름 등으로 나타난다. 멜라닌은 tyrosine에서 DOPA를 거쳐 산화 중합반응에 의해 생성되는 고분자 물질로 인체 내에 존재하는 머리카락 및 피부 색을 결정한다. 이러한 멜라닌은 melanocyte에서 합성되며, 활성산소에 의해 과도하게 생성되면 노화를 일으킨다는 연구가 보고되고 있다. 본 연구의 목적은 항산화 효과 및 멜라닌 합성에 대한 바나나 껍질 추출물(MPEE)의 직접적인 효과를 밝히는 것이다. MPEE는 DPPH radical scavenging assay와 reducing power assay를 수행한 결과, 두 실험 모두 양성대조군인 vitamin C와 비슷한 항산화 활성을 나타내었다. 세포 실험에 앞서 세포 독성을 알아보기 위해 B16F1 세포에서 MTT assay를 수행하였다. MPEE는 $32{\mu}g/ml$ 이하의 농도에서 세포독성이 없는 것으로 나타났다. 또한, MPEE는 invitro에서 tyrosinase 활성과 DOPA-oxidation 뿐만 아니라 살아있는 세포에서 멜라닌 합성을 증가시켰다. 더욱이, $H_2O_2$로 세포를 노화시켜 L-DOPA 실험을 수행한 결과, MPEE는 멜라닌 합성을 증가시켰다. 단백질 수준의 발현을 위한 Western blot 분석을 수행한 결과, TRP-1, TPR-2와 SOD-2의 발현 수준은 MPEE의 존재 하에서 증가되었다. 이상의 결과는 MPEE가 항산화 소재로 멜라닌 합성을 촉진시킨다는 것을 암시하고 있다.

Aging is accompanied by changes in the body, such as graying hair, wrinkles, and black spots composed of lipid peroxides and proteins. Melanin is a polymer substance produced by an oxidation polymerization reaction from tyrosine, and it determines the color of hair and skin. It has been reported that melanin is synthesized by melanocyte, and its excessive production by reactive oxygen species is associated with aging. The purpose of this study was to determine the direct effects of Musa paradisiaca peel ethanolic extract (MPEE) on antioxidative activity and melanin synthesis. It was observed that the antioxidant activity of MPEE was similar to that of vitamin C, a positive control, in both DPPH radical scavenging assay and reducing power assay. In order to examine cytotoxicity prior to cell experimentation, 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed for B16F1 cells. MPEE was not cytotoxic at $32{\mu}g/ml$ or less. In addition, MPEE increased melanin synthesis in live cells in addition to tyrosinase activity and melanin synthesis in dihydroxyphenylalanine (DOPA)-oxidation assay in vitro. Moreover, MPEE increased melanin synthesis in cells aged by pretreatment with $H_2O_2$. The expression levels of tyrosinase-related protein (TRP)-1, TRP-2, and superoxide dismutase (SOD)-2 by western blot analysis were increased in the presence of MPEE. These results suggest that MPEE could promote the melanin synthesis as an antioxidative substance.

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