Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Anti-obesity Effect of Ethanol Extracts from Silkworm (Bombyx mori) Pupae Powder Fermented with Cordyceps militaris in the Primary Adipocytes and High Fat Diet-induced Obesity Model Mice

일차 지방세포와 고지방식이로 유발한 비만모델동물에서 Cordyceps militaris로 발효시킨 누에 (Bombyx mori) 가루 에탄올 추출물의 항비만 효과

  • Kim, Ji Eun (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Lee, Mi Rim (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Choi, Jun Young (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Park, Jin Ju (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Kim, Hye Ryeong (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Song, Bo Ram (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Choi, Young Whan (Department of Horticultural Bioscience, College of Natural Resources & Life Science, Pusan National University) ;
  • Kim, Kyung Mi (Novarex Co.) ;
  • Hwang, Dae Youn (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University)
  • 김지은 (부산대학교 생명자원과학대학 바이오소재과학과 & 생명융합연구센터) ;
  • 이미림 (부산대학교 생명자원과학대학 바이오소재과학과 & 생명융합연구센터) ;
  • 최준영 (부산대학교 생명자원과학대학 바이오소재과학과 & 생명융합연구센터) ;
  • 박진주 (부산대학교 생명자원과학대학 바이오소재과학과 & 생명융합연구센터) ;
  • 김혜령 (부산대학교 생명자원과학대학 바이오소재과학과 & 생명융합연구센터) ;
  • 송보람 (부산대학교 생명자원과학대학 바이오소재과학과 & 생명융합연구센터) ;
  • 최영환 (부산대학교 생명자원과학대학 원예생명과학과) ;
  • 김경미 ((주)노바렉스) ;
  • 황대연 (부산대학교 생명자원과학대학 바이오소재과학과 & 생명융합연구센터)
  • Received : 2018.03.20
  • Accepted : 2018.05.27
  • Published : 2018.07.30
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Abstract

Silkworm (Bombyx mori) pupae have been widely worked in wound dressing, hepatoprotective activity, antigenotoxicity, control of glucose level and anti-cancer activity. To investigate the anti-obesity activity of ethanol extract of silkworm pupae powder fermented with Cordyceps militaris (ESfC), the free glycerol release and cAMP concentration as well as fat accumulation were measured in the primary adipocytes of SD (Sprague Dawley) rats and high fat diet (HFD)-treated C57BL/6 mice treated with 12 weeks. Firstly, the presence of the cordycepin with lipid lowering effect was confirmed in ESfC using HPLC analysis. The level of free glycerol and cAMP concentration was significantly increased in the primary adipocytes treated with high dose of ESfC ($400{\mu}g/ml$) although these levels were consistently maintained in other dose ESfC treated groups. In HFD-induced obesity model, the increased fat weight and size of adipocytes in HFD+Vehicle treated group was recovered in HFD+ESfC treated group. Also, the liver weight and the number of lipid droplets were higher in HFD+Vehicle treated group than No treated group. But, this level was significantly decreased in HFD+ESfC treated group compared with HFD+Vehicle treated group. Furthermore, a similar recovery was detected on the phosphorylation of periliphin and HSL, and ATGL expression. Overall, the results of the present study provide some scientific evidences that ESfC can stimulate lipolysis in primary adipocytes and prevent fat accumulation in HFD-treated obesity model, and therefore have the potential for use as anti-obesity agents to treat obese patient.

누에(Bombyx mori)번데기는 창상치료용 드레싱, 간보호활성, 항유전독성, 포도당농도 조절, 항암활성 등에 다양하게 적용되고 있다. 본 연구에서는 붉은동충하초(Cordyceps militaris)를 이용하여 발효시킨 누에번데기분말의 에탄올 추출물(Ethanol extract of silkworm pupae fermented with Cordyceps militaris, ESfC)의 항비만효능을 분석하기 위하여, SD랫드로부터 분리된 일차지방세포(Primary adipocytes)에서 농도별로 ESfC를 처리하여 free glycerol release, cAMP농도를 측정하였고, 고지방사료(High fat diet, HFD)가 처리된 C57BL/6N 비만마우스에 ESfC를 투여하여 지방조직과 간조직에서 지방축적(Fat accumulation)을 관찰하였다. 먼저, 지방감소효과를 나타내는 지표물질로서 cordycepin의 존재는 HPLC분석을 통하여 ESfC에서 확인하였다. 또한, free glycerol수준과 cAMP농도는 고농도 ESfC가 처리된 일차지방 세포에서 유의적으로 증가하였지만 이들 수준은 저농도와 중농도의 ESfC로처리세포에서는 유의적인 변화가 없었다. HFD-유도 비만모델에서, 고지방사료의 투여에 의해 유의적으로 증가된 지방무게와 지방세포의 크기는 ESfC처리그룹에서 감소되었다. 간 무게와 지방방울(Lipid droplet)의 수는 고지방사료의 투여에 의해 증가하였지만 ESfC투여에 의해 유의적으로 감소하였다. 또한, 유사한 회복효과는 perilipin과 HSL의 인산화 수준뿐만 아니라 ATGL의 발현수준에서도 관찰되었다. 따라서 이상의 결과는 ESfC가 지방일차세포에서 지방분해를 촉진하고, HFS유도 비만모델에서 지방 축적을 억제하는 효과를 나타냄을 보여주고 있으며, 향후 비만환자에 처리하기 위한 약물로서 사용가능성을 제시하고 있다.

Keywords

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