대한본초학회지 (The Korea Journal of Herbology)

  • 제33권4호
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  • Pages.101-108
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  • 2018
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
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진피(陳皮)의 항산화 활성 및 L6 근육세포에서 당대사에 미치는 영향

Antioxidant Activity of Citrus Peel and Effect on its Glucose Metabolism in L6 Rat Skeletal Muscle Cells

  • 김수현 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 박해진 (대구한의대학교 산학협력단 코스메디컬센터) ;
  • 김경조 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김민주 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이진아 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이아름 (호산대학교 뷰티디자인학과) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실)
  • Kim, Soo Hyun (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Park, Hae-Jin (Cosmedical Center, Academic Industry Cooperation, Daegu Haany University) ;
  • Kim, Kyeong Jo (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Min Ju (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Jin A (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Ah Reum (Department of beauty design, Hosan university) ;
  • Roh, Seong-Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
  • 투고 : 2018.06.22
  • 심사 : 2018.07.25
  • 발행 : 2018.07.30
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초록

Objectives : This study aimed to effects antioxidant activity of citrus peel extract (CPE) and effect on its glucose metabolism in L6 rat skeletal muscle cells. Methods : Antioxidative activities were evaluated by using 10 kinds of natural materials, and total polyphenol and flavonoid contents were examined. The L6 muscle cells toxicity of CPE was examined by MTT assay. Expression of glucose-related genes in L6 muscle cells by CPE treatment was analyzed by real-time PCR and western blotting. Results : The $IC_{50}$ values of DPPH and ABTS free radical scavenging activity of CPE were ($15.47{\pm}0.26{\mu}g/m{\ell}$ and $12.07{\pm}1.23{\mu}g/m{\ell}$, respectively), effectively clearing DPPH and ABTS. CPE showed total polyphenol and flavonoid contents ($20.30{\pm}0.38$ and $64.20{\pm}0.52$, respectively). The selected CPE were used in experiments using an effective concentration that is not toxic in L6 muscle cells. We investigated insulin receptor substrate-1 (IRS-1), phosphatidylinositol 3-kinase regulatory (PI3KR), Akt, and glucose transporter 4 (GLUT4). mRNA analysis by realtime PCR showed no significant difference, but CPE-treated cells showed a tendency to increase in concentration-dependent manner. However, analysis of protein expression of Akt and GLUT4 by western blotting showed that CPE treatment significantly increased concentration dependent (p<0.001). Conclusions : As a result, citrus peel extract with high antioxidant activity regulates glucose metabolism in L6 muscle cells. Therefore, CPE can be a potential treatment for the treatment of diabetes.

키워드

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