The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effects of hexane fraction of Dracocephalum palmatum Stephan leaf on human-derived prostate cancer cell death

Dracocephalum palmatum Stephan 잎 헥산 분획 추출물의 인간 유래 전립선 암세포 사멸에 대한 작용

  • Lee, Min Ji (Department of Korean Medical Science, School of Korean Medicine, Pusan National University) ;
  • Lee, Se-Eun (Department of Korean Medical Science, School of Korean Medicine, Pusan National University) ;
  • Choi, Na Ri (Department of Korean Medicine, School of Korean Medicine, Pusan National University) ;
  • Jo, Sung Hyeon (Department of Korean Medicine, School of Korean Medicine, Pusan National University) ;
  • Cho, Suin (Department of Korean Medicine, School of Korean Medicine, Pusan National University)
  • 이민지 (부산대학교 한의학전문대학원 한의과학과) ;
  • 이세은 (부산대학교 한의학전문대학원 한의과학과) ;
  • 최나리 (부산대학교 한의학전문대학원 한의학과) ;
  • 조성현 (부산대학교 한의학전문대학원 한의학과) ;
  • 조수인 (부산대학교 한의학전문대학원 한의학과)
  • Received : 2018.06.01
  • Accepted : 2018.07.25
  • Published : 2018.07.30
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Abstract

Objectives : Dracocephalum palmatum Stephan (Lamiaceae) is a medicinal plant used by the East-Russian nomads but there were few studies on this plant. This study was to evaluate anti-cancer effects of D. palmatum Stephan leaf hexane fraction on human derived prostate cancer cell death. Methods : The dried leaves of D. palmatum were dissolved in methanol, and hexane fraction (DpLH) was again obtained from lyophilized methanol extract (DpLM). DpLH was investigated by measuring by MTT assay and annexin V/PI staining to evaluate its effects on the cell viability and apoptosis of PC-3 cells. The ROS generations were detected by DCF-DA dye. The protein expressions were confirmed by p-AKT, Bcl-2, Bax, procaspase-3 activities. Results : After treatment of DpLH to PC-3 cells, the cell proliferation was significantly inhibited, and in addition, DpLH treatment also accelerated apoptosis of PC-3 cells. When DpLH was treated to the PC-3 cells, its ROS production significantly decreased. The proportion of all proteins (p-AKT/actin, Bcl-2/Bax and procaspase-3/actin ratios) showed decreasing tendency of expression compared with the control group. Conclusions : As shown in the above results, the extract from D. palmatum inhibits ROS production and promotes cell death, which is considered to be a relatively safe induction of cell death when administered to a living body. In conclusion, these results suggested that DpLH may have anti-cancer effect in human prostate cancer cell.

Keywords

References

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