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A molecular investigation of Saccharina sessilis from the Aleutian Islands reveals a species complex, necessitating the new combination Saccharina subsessilis

  • Starko, Samuel (Department of Botany & Biodiversity Research Centre, The University of British Columbia) ;
  • Boo, Ga Hun (University Herbarium, University of California) ;
  • Martone, Patrick T. (Department of Botany & Biodiversity Research Centre, The University of British Columbia) ;
  • Lindstrom, Sandra C. (Department of Botany & Biodiversity Research Centre, The University of British Columbia)
  • 투고 : 2018.01.20
  • 심사 : 2018.04.02
  • 발행 : 2018.06.15

초록

Cryptic species complexes are increasingly recognized in phycological research, obscuring taxonomy and raising questions about factors influencing speciation. A recent exploration of kelp genetic diversity on Haida Gwaii, British Columbia revealed the existence of a new species, Saccharina druehlii, which is cryptic with Saccharina sessilis. This suggests that molecular investigations further north may be required to elucidate the taxonomy and evolutionary history of this lineage. Although, for several decades, S. sessilis was considered a single highly variable species, its taxonomy has been far from straightforward. In particular, Hedophyllum subsessile (Areschoug) Setchell is now recognized as a synonym of S. sessilis in North America, but as a growth form of Saccharina bongardiana in Far East Russia. To resolve this taxonomic confusion, we sequenced mitochondrial (CO1-5P) and nuclear (internal transcribed spacer) markers of S. sessilis populations from the Aleutian Islands, Alaska, USA. Interestingly, none of our sequences matched S. sessilis sensu stricto. Instead, CO1-5P sequences from populations in the central and eastern Aleutians matched exactly S. druehlii with increasing sequence divergence occurring westward. Samples from Attu, the western-most island, composed a genetic group that clearly represents Kjellman's concept of Hafgygia bongardiana f. subsessilis and is distinct enough from S. druehlii and S. sessilis to potentially constitute a distinct species. Therefore, Saccharina subsessilis comb. nov. is proposed for this entity. Our results suggest the existence of a species complex at the crown node of S. sessilis and thus further investigation of Saccharina in Alaskan waters should be conducted to reconstruct the evolutionary history of this fascinating lineage.

키워드

참고문헌

  1. Areschoug, J. E. 1883. Obscrvationes phycologicae. IV. Nova Acta Regiae Soc. Sci. Upsaliensis Ser. III 12:5.
  2. Augyte, S., Lewis, L., Lin, S., Neefus, C. D. & Yarish, C. 2018. Speciation in the exposed intertidal zone: the case of Saccharina angustissima comb. nov. & stat. nov. (Laminariales, Phaeophyceae). Phycologia 57:100-112. https://doi.org/10.2216/17-40.1
  3. Bartsch, I., Wiencke, C., Bischof, K., Buckholz, C. M., Buck, B. H., Eggert, A., Feuerpfeil, P., Hanelt, D., Jacobsen, S., Karez, R., Karsten, U., Molis, M., Roleda, M. Y., Schubert, H., Schumann, R., Valentin, K., Weinberger, R. & Wiese, J. 2008. The genus Laminaria sensu lato: recent insights and developments. Eur. J. Phycol. 43:1-86. https://doi.org/10.1080/09670260701711376
  4. Bolton, J. J. 2010. The biogeography of kelps (Laminariales, Phaeophyceae): a global analysis with new insights from recent advances in molecular phylogenetics. Helgol. Mar. Res. 64:263-279. https://doi.org/10.1007/s10152-010-0211-6
  5. Boo, G. H., Lindstrom, S. C., Klochkova, N. G., Yotsukura, N., Yang, E. C., Kim, H. G., Waaland, J. R., Cho, G. Y., Miller, K. A. & Boo, S. M. 2011. Taxonomy and biogeography of Agarum and Thalassiophyllum (Laminariales, Phaeophyceae) based on sequences of nuclear, mitochondrial, and plastid markers. Taxon 60:831-840.
  6. Bouckaert, R., Heled, J., Kuhnert, D., Vaughan, T., Wu, C. -H., Xie, D., Suchard, M. A., Rambaut, A. & Drummond, A. J. 2014. BEAST 2: a software platform for Bayesian evolutionary analysis. PLoS Comput. Biol. 10:e1003537. https://doi.org/10.1371/journal.pcbi.1003537
  7. Fujisawa, T. & Barraclough, T. G. 2013. Delimiting species using single-locus data and the Generalized Mixed Yule Coalescent approach: a revised method and evaluation on simulated data sets. Syst. Biol. 62:707-724. https://doi.org/10.1093/sysbio/syt033
  8. Joly, S., McLenachan, P. A. & Lockhart, P. J. 2009. A statistical approach for distinguishing hybridization and incomplete lineage sorting. Am. Nat. 174:E54-E70. https://doi.org/10.1086/600082
  9. Katoh, K., Misawa, K., Kuma, K. & Miyata, T. 2002. MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res. 30:3059-3066. https://doi.org/10.1093/nar/gkf436
  10. Kjellman, F. R. 1889. Om Beringhafvets Algflora. Kgl. Svenska Vetenskapsakad Handl. 23:1-55.
  11. Klochkova, N. G., Korolyova, T. N. & Kusidi, A. E. 2009. Marine algae of Kamchatka and surrounding areas. Vol. 1. KamchatNIRO Press, Petropavlovsk-Kamchatskii, 218pp. (in Russian).
  12. Lane, C. E., Lindstrom, S. C. & Saunders, G. W. 2007. A molecular assessment of northeast Pacific Alaria species (Laminariales, Phaeophyceae) with reference to the utility of DNA barcoding. Mol. Phylogenet. Evol. 44:634-648. https://doi.org/10.1016/j.ympev.2007.03.016
  13. Lee, K. M., Boo, G. H., Riosmena-Rodriguez, R., Shin, J. -A. & Boo, S. M. 2009. Classification of the genus Ishige (Ishigeales, Phaeophyceae) in the North Pacific Ocean with recognition of Ishige foliacea based on plastid rbcL and mitochondrial cox3 gene sequences. J. Phycol. 45:906-913. https://doi.org/10.1111/j.1529-8817.2009.00704.x
  14. Lindstrom, S. C. 2009. The biogeography of seaweeds in Southeast Alaska. J. Biogeogr. 36:401-409. https://doi.org/10.1111/j.1365-2699.2007.01855.x
  15. Lindstrom, S. C., Lindeberg, M. R. & Guthrie, D. A. 2015. Marine macroalgae of the Aleutian Islands: I. Bangiales. Algae 30:247-263. https://doi.org/10.4490/algae.2015.30.4.247
  16. Maddison, W. P. & Knowles, L. L. 2006. Inferring phylogeny despite incomplete lineage sorting. Syst. Biol. 55:21-30. https://doi.org/10.1080/10635150500354928
  17. McCarthy, C. 1998. Chromas, version 1.45. School of Health Science, Griffith University, Southport, Queensland, Australia.
  18. McDevit, D. C. & Saunders, G. W. 2010. A DNA barcode examination of the Laminariaceae (Phaeophyceae) in Canada reveals novel biogeographical and evolutionary insights. Phycologia 49:235-248. https://doi.org/10.2216/PH09-36.1
  19. McNeill, J., Barrie, F. R., Buck, W. R., Demoulin, V., Greuter, W., Hawksworth, D. L., Herendeen, P. S., Knapp, S., Marhold, K., Prado, J., Prud'homme van Reine, W. F., Smith, G. F., Wiersema, J. H. & Turland, N. J. 2012. International Code of Nomenclature for algae, fungi, and plants (Melbourne Code). Regnum Vegetabile 154. Koeltz Scientific Books, Konigstein, 208 pp.
  20. Miller, K. A., Olsen, J. L. & Stam, W. T. 2000. Genetic divergence correlates with morphological and ecological subdivision in the deep‐water elk kelp, Pelagophycus porra (Phaeophyceae). J. Phycol. 36:862-870. https://doi.org/10.1046/j.1529-8817.2000.99233.x
  21. Nielsen, M. M., Paulino, C., Neiva, J., Krause-Jensen, D., Bruhn, A. & Serrao, E. A. 2016. Genetic diversity of Saccharina latissima (Phaeophyceae) along a salinity gradient in the North Sea-Baltic Sea transition zone. J. Phycol. 52:523-531. https://doi.org/10.1111/jpy.12428
  22. Pollard, D. A., Iyer, V. N., Moses, A. M. & Eisen, M. B. 2006. Widespread discordance of gene trees with species tree in Drosophila: evidence for incomplete lineage sorting. PLoS Genet. 2:e173. https://doi.org/10.1371/journal.pgen.0020173
  23. Rai, H. S., O'Brien, H. E., Reeves, P. A., Olmstead, R. G. & Graham, S. W. 2003. Inference of higher-order relationships in the cycads from a large chloroplast data set. Mol. Phylogenet. Evol. 29:350-359. https://doi.org/10.1016/S1055-7903(03)00131-3
  24. Saunders, D. A. 1901. Papers from the Harriman Alaska Expedition. XXV. The algae. Proc. Wash. Acad. Sci. 3:391-486.
  25. Saunders, G. W. 2005. Applying DNA barcoding to red macroalgae: a preliminary appraisal holds promise for future applications. Philos. Trans. R. Soc. B Biol. Sci. 360:1879-1888. https://doi.org/10.1098/rstb.2005.1719
  26. Saunders, G. W. & McDevit, D. C. 2014. A DNA barcode survey of Haida Gwaii kelp (Laminariales, Phaeophyceae) reveals novel ecological and distributional observations and Saccharina druehlii sp. nov. Botany 92:821-826. https://doi.org/10.1139/cjb-2014-0119
  27. Selivanova, O. N., Zhigadlova, G. G. & Hansen, G. I. 2007. Revision of the systematics of algae in the order Laminariales (Phaeophyta) from the Far-Eastern seas of Russia on the basis of molecular-phylogenetic data. Russ. J. Mar. Biol. 33:278-289. https://doi.org/10.1134/S1063074007050021
  28. Setchell, W. A. 1901. Notes on algae, I. Zoe 5:121-129.
  29. Stabeno, P. J., Kachel, D. G., Kachel, N. B. & Sullivan, M. E. 2005. Observations from moorings in the Aleutian Passes: temperature, salinity and transport. Fish. Oceanogr. 14(Suppl. 1):39-54. https://doi.org/10.1111/j.1365-2419.2005.00362.x
  30. Stamatakis, A. 2014. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30:1312-1313. https://doi.org/10.1093/bioinformatics/btu033
  31. Tellier, F., Tapai, J., Faugero, S., Destombe, C. & Valero, M. 2011a. The Lessonia nigrescens species complex (Laminariales, Phaeophyceae) shows strict paraptry and complete reproductive isolation in a secondary contact zone. J. Phycol. 47:894-903. https://doi.org/10.1111/j.1529-8817.2011.01019.x
  32. Tellier, F., Vega, J. M. A., Broitman, B. R., Vasquez, J. A., Valero, M. & Faugeron, S. 2011b. The importance of having two species instead of one in kelp management: the Lessonia nigrescens species complex. Cah. Biol. Mar. 52:455-465.
  33. Widdowson, T. B. 1965. A taxonomic study of the genus Hedophyllum Setchell. Can. J. Bot. 43:1409-1420. https://doi.org/10.1139/b65-150
  34. Wynne, M. J. 2006. Portraits of marine algae: an historical perspective. University of Michigan Herbarium, Ann Arbor, MI, 180 pp.
  35. Yendo, K. 1903. Hedophyllum spirale sp. nov., and its relation to Thalassiophyllum and Arthrothamnus. Bot. Mag (Tokyo) 17:165-173. https://doi.org/10.15281/jplantres1887.17.201_165

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