한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제46권2호
  • /
  • Pages.114-119
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  • 2018
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Enhanced Production of Astaxanthin by Metabolically Engineered Non-mevalonate Pathway in Escherichia coli

  • Jeong, Tae Hyug (Department of Marine and Fisheries Resources, Mokpo National University) ;
  • Cho, Youn Su (Department of Fishery Biology, Pukyong National University) ;
  • Choi, Seong-Seok (Department of Microbiology, Pukyong National University) ;
  • Kim, Gun-Do (Department of Microbiology, Pukyong National University) ;
  • Lim, Han Kyu (Department of Marine and Fisheries Resources, Mokpo National University)
  • 투고 : 2018.01.11
  • 심사 : 2018.03.16
  • 발행 : 2018.06.28
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초록

Astaxanthin is one of the major carotenoids used in pigment has a great economical value in pharmaceutical markets, feeding, nutraceutical and food industries. This study was to increase the production of astaxanthin by co-expression with transformed Escherichia coli using six genes involved in the non-mevalonate pathway. Involved in the non-mevalonate biosynthetic pathway of the strain Kocuria gwangalliensis were cloned dxs, ispC, ispD, ispE, ispF, ispG, ispH and idi genes in order to increase astaxanthin production from the transformed E. coli. And co-expression with the genes to compared the amount of astaxanthin production. This engineered E. coli, containing both the non-mevalonate pathway gene and the astaxanthin biosynthesis gene cluster, produced astaxanthin at $1,100{\mu}g/g$ DCW (dry cell weight), resulting in approximately three times the production of astaxanthin.

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참고문헌

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