Journal of Korean Neurosurgical Society

  • 제61권4호
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  • Pages.434-440
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  • 2018
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  • 2005-3711(pISSN)
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  • 1598-7876(eISSN)
대한신경외과학회 (The Korean Neurosurgical Society)
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In Vivo Spinal Distribution of Cy5.5 Fluorescent Dye after Injection via the Lateral Ventricle and Cisterna Magna in Rat Model

  • Lee, Kee-Hang (Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University) ;
  • Nam, Hyun (Single Cell Network Research Center, Sungkyunkwan University School of Medicine) ;
  • Won, Jeong-Seob (Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University) ;
  • Hwang, Ji-Yoon (Single Cell Network Research Center, Sungkyunkwan University School of Medicine) ;
  • Jang, Hye Won (Department of Medical Education, Sungkyunkwan University School of Medicine) ;
  • Lee, Sun-Ho (Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University) ;
  • Joo, Kyeung Min (Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University)
  • 투고 : 2017.09.18
  • 심사 : 2017.10.23
  • 발행 : 2018.07.01
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초록

Objective : The purpose of this study was to find an optimal delivery route for clinical trials of intrathecal cell therapy for spinal cord injury in preclinical stage. Methods : We compared in vivo distribution of Cy5.5 fluorescent dye in the spinal cord region at various time points utilizing in vivo optical imaging techniques, which was injected into the lateral ventricle (LV) or cisterna magna (CM) of rats. Results : Although CM locates nearer to the spinal cord than the LV, significantly higher signal of Cy5.5 was detected in the thoracic and lumbar spinal cord region at all time points tested when Cy5.5 was injected into the LV. In the LV injection Cy5.5 signal in the thoracic and lumbar spinal cord was observed within 12 hours after injection, which was maintained until 72 hours after injection. In contrast, Cy5.5 signal was concentrated at the injection site in the CM injection at all time points. Conclusion : These data suggested that the LV might be suitable for preclinical injection route of therapeutics targeting the spinal cord to test their treatment efficacy and biosafety for spinal cord diseases in small animal models.

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참고문헌

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피인용 문헌

  1. In vivo distribution of U87MG cells injected into the lateral ventricle of rats with spinal cord injury vol.13, pp.8, 2018, https://doi.org/10.1371/journal.pone.0202307