International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Differentiation of CD31-Positive Vascular Endothelial Cells from Organoid Culture of Dental Pulp Stem Cells

  • Seo, Eun Jin (Department of Oral Biochemistry and Molecular Biology, Pusan National University School of Dentistry) ;
  • Park, Jae Kyung (Department of Oral Biochemistry and Molecular Biology, Pusan National University School of Dentistry) ;
  • Jeong, Hoim (Department of Oral Biochemistry and Molecular Biology, Pusan National University School of Dentistry) ;
  • Kang, Jung Sook (Department of Oral Biochemistry and Molecular Biology, Pusan National University School of Dentistry) ;
  • Kim, Hyung-Ryong (DGIST) ;
  • Jang, Il Ho (Department of Oral Biochemistry and Molecular Biology, Pusan National University School of Dentistry)
  • Received : 2018.06.11
  • Accepted : 2018.06.22
  • Published : 2018.06.30
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Abstract

The mesenchymal stem cells (MSCs) that reside in dental tissues hold a great potential for future applications in regenerative dentistry. In this study, we used human dental pulp cells, isolated from the molars (DPCs), in order to establish the organoid culture. DPCs were established after growing pulp cells in an MSC expansion media (MSC-EM). DPCs were subjected to organoid growth media (OGM) in comparison with human dental pulp stem cells (DPSCs). Inside the extracellular matrix in the OGM, the DPCs and DPSCs readily formed vessel-like structures, which were not observed in the MSC-EM. Immunocytochemistry analysis and flow cytometry analysis showed the elevated expression of CD31 in the DPCs and DPSCs cultured in the OGM. These results suggest endothelial cell-prone differentiation of the DPCs and DPSCs in organoid culture condition.

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References

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