The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Protective effect of furosin isolated from Euphorbia helioscopia against glutamate-induced HT22 cell death

등대(燈臺)풀 유래 Furosin의 glutamate에 의한 HT22 세포 사멸 억제 효과

  • Baek, Ji Yun (Kyeongnam National University of Science and Technology) ;
  • Song, Ji Hoon (College of Korean Medicine, Gachon University) ;
  • Choi, Sung Youl (Department of Oriental Neuropsychiatry, College of Korean Medicine, Gachon University)
  • 백지윤 (경남과학기술대학교) ;
  • 송지훈 (가천대학교 한의과대학) ;
  • 최성열 (가천대학교 한의과대학 한방신경정신과)
  • Received : 2017.02.13
  • Accepted : 2018.03.14
  • Published : 2018.03.31
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Abstract

Objectives: In the brain, glutamate is the most important excitable neurotransmitter in physiological and pathological conditions. However, the high level of glutamate induces neuronal cell death due to exitotoxicity and oxidative stress. The present study investigated to evaluate a possible neuroprotective effect of furosin isolated from Euphorbia helioscopia against glutamate-induced HT22 cell death. Methods: Furosin was isolated from methanol extract of Euphorbia helioscopia and examined whether it protects glutamate-induced neuronal cell death. The cell viability was determined using Ez-Cytox assay. Anti-oxidative effect of furosin was determined by DPPH scavenging activities, and the levels of intracellular reactive oxygen species (ROS) were determined by the fluorescent intensity after staining the cells with $H_2DCFDA$. To evaluate apoptotic cell death, we performed nuclear staining and image-based cytometeric analysis. Results: The cell viability was significantly increased by treatement with furosin compared with the treatment with glutamate. Furosin showed a strong DPPH radical scavenging activity ($EC50=1.83{\mu}M$) and prevented the accumulation of intra cellular ROS. Finally, the presence of 50 and $100{\mu}M$ furosin significantly the percentage of apoptotic cells compared with glutamate treatment. Conclusion: The present study found that furosin is a potent neuroprotectant against glutamate-induced oxidative stress through inhibition of apoptotic cell death induced by glutamate. Therefore, the present study suggests that furosin as a bioactive compound of E. helioscopia can be a useful source to develop a drug for the treatment of neurodegenerative diseases and acute brain injuries.

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References

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