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사람 치주염 병소의 치은연하지면세균막에서 분리된 Fusobacterium animalis KCOM 1280의 유전체 염기서열 해독

Draft genome sequence of Fusobacterium animalis KCOM 1280 isolated from a human subgingival plaque of periodontitis lesion

  • 박순낭 (조선대학교 치과대학 구강생화학교실 및 한국구강미생물자원은행) ;
  • 임윤경 (조선대학교 치과대학 구강생화학교실 및 한국구강미생물자원은행) ;
  • 신자영 (마크로젠) ;
  • 노한성 (마크로젠) ;
  • 국중기 (조선대학교 치과대학 구강생화학교실 및 한국구강미생물자원은행)
  • Park, Soon-Nang (Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University) ;
  • Lim, Yun Kyong (Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University) ;
  • Shin, Ja Young (Macrogen Inc.) ;
  • Roh, Hanseong (Macrogen Inc.) ;
  • Kook, Joong-Ki (Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University)
  • 투고 : 2017.12.12
  • 심사 : 2018.02.06
  • 발행 : 2018.06.30

초록

Fusobacterium animalis (예전에 Fusobacterium nucleatum subsp. animalis으로 알려짐)는 그람 음성이면서, 혐기성 및 선형의 세균이다. F. animalis는 사람 구강 내 정상 세균총의 하나이며 치주질환원인균이라 여겨지고 있다. F. animalis KCOM 1280 (= ChDC F318) 균주는 사람 치주질환 병소에서 분리되었다. 본 논문에서 F. animalis KCOM 1280 균주 유전체 염기서열을 해독하여 보고하고자 한다.

Fusobacterium animalis (formerly Fusobacterium nucleatum subsp. animalis) is a Gram-negative, anaerobic, and filament-shaped bacterium. F. animalis may be a part of normal flora and a periodontopathogen of human oral cavity. F. animalis KCOM 1280 (= ChDC F318) was isolated from a human periodontitis lesion. In this report, we present the draft genome sequence of F. animalis KCOM 1280.

키워드

참고문헌

  1. Boetzer, M. and Pirovano, W. 2014. SSPACE-LongRead: scaffolding bacterial draft genomes using long read sequence information. BMC Bioinformatics 15, 211. https://doi.org/10.1186/1471-2105-15-211
  2. Cho, E., Park, S.N., Lim, Y.K., Shin, Y., Paek, J., Hwang, C.H., Chang, Y.H., and Kook, J.K. 2015. Fusobacterium hwasookii sp. nov., isolated from a human periodontitis lesion. Curr. Microbiol. 70, 169-175. https://doi.org/10.1007/s00284-014-0692-7
  3. Gharbia, S.E. and Shah, H.N. 1990. Heterogeneity within Fusobacterium nucleatum, proposal of four subspecies. Lett. Appl. Microbiol. 10, 105-108. https://doi.org/10.1111/j.1472-765X.1990.tb00276.x
  4. Gharbia, S.E. and Shah, H.N. 1992. Fusobacterium nucleatum subsp. fusiforme subsp. nov. and Fusobacterium nucleatum subsp. animalis subsp. nov. as additional subspecies within Fusobacterium nucleatum. Int. J. Syst. Bacteriol. 42, 296-298. https://doi.org/10.1099/00207713-42-2-296
  5. Gnerre, S., Maccallum, I., Przybylski, D., Ribeiro, F.J., Burton, J.N., Walker, B.J., Sharpe, T., Hall, G., Shea, T.P., Sykes, S., et al. 2011. High-quality draft assemblies of mammalian genomes from massively parallel sequence data. Proc. Natl. Acad. Sci. USA 108, 1513-1518. https://doi.org/10.1073/pnas.1017351108
  6. Kook, J.K., Park, S.N., Lim, Y.K., Cho, E., Jo, E., Roh, H., Shin, Y., Paek, J., Kim, H.S., Kim, H., et al. 2017. Genome-based reclassification of Fusobacterium nucleatum subspecies at the species level. Curr. Microbiol. 74, 1137-1147. https://doi.org/10.1007/s00284-017-1296-9
  7. Luo, R., Liu, B., Xie, Y., Li, Z., Huang, W., Yuan, J., He, G., Chen, Y., Pan, Q., Liu, Y., et al. 2012. SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler. Gigascience. 1, 18. Erratum in: Gigascience. 2015. 4, 30. https://doi.org/10.1186/2047-217X-1-18
  8. Otto, T.D., Sanders, M., Berriman, M., and Newbold, C. 2010. Iterative correction of reference nucleotides (iCORN) using second generation sequencing technology. Bioinformatics 26, 1704-1707. https://doi.org/10.1093/bioinformatics/btq269
  9. Park, S.N., Lim, Y.K., and Kook, J.K. 2013. Development of quantitative real-time PCR primers for detecting 42 oral bacterial species. Arch. Microbiol. 195, 473-482. https://doi.org/10.1007/s00203-013-0896-4