Asian-Australasian Journal of Animal Sciences

  • 제31권8호
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  • Pages.1119-1126
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  • 2018
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Luteinizing hormone beta gene polymorphism and its effect on semen quality traits and luteinizing hormone concentrations in Murrah buffalo bulls

  • Reen, Jagish Kour (Genetics Laboratory, Dairy Production Section, ICAR - National Dairy Research Institute) ;
  • Kerekoppa, Ramesha (Genetics Laboratory, Dairy Production Section, ICAR - National Dairy Research Institute) ;
  • Deginal, Revanasiddu (Genetics Laboratory, Dairy Production Section, ICAR - National Dairy Research Institute) ;
  • Ahirwar, Maneesh Kumar (Livestock Research Centre, ICAR-National Dairy Research Institute) ;
  • Kannegundla, Uday (Genetics Laboratory, Dairy Production Section, ICAR - National Dairy Research Institute) ;
  • Chandra, Satish (Genetics Laboratory, Dairy Production Section, ICAR - National Dairy Research Institute) ;
  • Palat, Divya (Genetics Laboratory, Dairy Production Section, ICAR - National Dairy Research Institute) ;
  • Das, Dayal Nitai (Genetics Laboratory, Dairy Production Section, ICAR - National Dairy Research Institute) ;
  • Kataktalware, Mukund Amritrao (Livestock Research Centre, ICAR-National Dairy Research Institute) ;
  • Jeyakumar, Sakthivel (Livestock Research Centre, ICAR-National Dairy Research Institute) ;
  • Isloor, Shri krishna (Department of Microbiology, Veterinary College)
  • 투고 : 2017.09.10
  • 심사 : 2017.11.24
  • 발행 : 2018.08.01
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초록

Objective: Present investigation was aimed to study the Single Nucleotide Variants of the luteinizing hormone beta ($LH{\beta}$) gene and to analyze their association with the semen quality (fresh and post-thawed frozen semen) and luteinizing hormone (LH) concentrations in Murrah buffalo bulls. Methods: Polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) and Sanger sequencing method is used to study genetic variability in $LH{\beta}$ gene. LH assay was carried out using enzyme-linked immunosorbent assay method. A fixed general linear model was used to analyze association of single nucleotide polymorphism (SNP) of $LH{\beta}$ gene with semen quality in 109 and LH concentrations in 80 Murrah bulls. Results: $LH{\beta}$ gene was found to be polymorphic. Total six SNPs were identified in $LH{\beta}$ gene g C356090A, g C356113T, g A356701G, g G355869A, g G356330C, and g G356606T. Single Stranded Conformational Polymorphism variants of pattern 2 of exon 1+pattern 2 of exon 2+pattern 1 of exon 3 had highly significant (p<0.01) effect on sperm concentration (million/mL), percent mass motility, acrosome integrity and membrane integrity in fresh and frozen semen whereas significant (p<0.05) effect was observed on percent live spermatozoa. SSCP variants of pattern 2 of exon 1+pattern 2 of exon 2+pattern 1 of exon 3 had highly significant (p<0.01) effect on luteinizing hormone concentrations too. Conclusion: The observed association between SSCP variants of $LH{\beta}$ gene with semen quality parameters and LH concentrations indicated the possibilities of using $LH{\beta}$ as a candidate gene for identification of markers for semen quality traits and LH concentrations in Murrah buffaloes.

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참고문헌

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