Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Comparative Study of Anti-inflammatory and Immunological Activities by Different Gender and Parts of Yeonsan Ogye

연산오계의 성별과 부위별 항염증 및 면역 활성 비교 연구

  • Do, Young Min (Baedok saengki Korean Medicine) ;
  • Kim, Dong Hee (Department of Pathology, College of Korean Medicine, Daejeon University)
  • 도영민 (배독생기한의원) ;
  • 김동희 (대전대학교 한의과대학 병리학교실)
  • Received : 2018.01.08
  • Accepted : 2018.02.26
  • Published : 2018.04.25
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Abstract

The aim of this study is to compare the anti-inflammatory and immunological activity of different parts (bone, meat, and rind) of Yeonsan Ogye (YO). In order to evaluate cytotoxicity, MTT assay was performed. We investigated the production of nitric oxide (NO) and pro-inflammatory cytokines, such as IL-$1{\beta}$, IL-6, and TNF-${\alpha}$, in LPS-induced RAW264.7 cells. All parts of the YO showed no toxicity at concentrations of 1, 10, and $100{\mu}g/m{\ell}$. Rooster's bone, hen's bone, and rind decreased the production of NO. And rooster's bone, meat, and hen's bone also attenuated TNF-${\alpha}$ production in LPS-induced RAW 264.7 cells. In addition, all parts of the YO decreased IL-$1{\beta}$ and IL-6 production in LPS-induced RAW264.7 cells, whereas they all increased IL-$1{\beta}$, IL-6 and TNF-${\alpha}$ production in normal RAW264.7 cells. Rooster exhibited higher immune activation and inhibitory activity on inflammation than a hen, and among different parts of the YO, bone showed the highest activity. Our results demonstrated and compared the anti-inflammatory and immunological activity of different parts of the YO. These results suggest that YO may be developed as a raw material for new health supplement food and medicine to attenuate various symptoms related to inflammation and immunity.

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References

  1. Hibbs JB, Taintor RR, Vavrin Z, Rachlin EM. Nitric oxide: a cytotoxic activated macrophage effector molecule. Biochemical and biophysical research communications. 1988;157(1):87-94. https://doi.org/10.1016/S0006-291X(88)80015-9
  2. Nathan CF. Secretory products of macrophages. Journal of Clinical Investigation. 1987 Feb;79(2):319. https://doi.org/10.1172/JCI112815
  3. Kim HY, Hwang IG, Kim TM, Park DS, Kim JH, et al. Antioxidant and angiotensin converting enzyme I inhibitory activity on different parts of germinated rough rice. Journal of the Korean Society of Food Science and Nutrition. 2011;40(6).
  4. Kim JW, Sim BY, Choi HJ, Lee HJ, Kim DH. The Study on Biological Activities of Yeonsan Ogye listed on Dong-ui-bo-gam. The Korea Journal of Herbology. 2015;30(5):23-8. https://doi.org/10.6116/kjh.2015.30.5.23.
  5. Heo, J. DongEuiBoGam. DongEuiBoGamBooks. 2006;2025-6.
  6. Chae HS, Ahn CN, Yoo YM, Ham JS, Lee JM et al. The Effects of the High Pressure Boiled Extracts (HPBE) of the Ogol Chicken with Herbs on the Hormones, Cytokine, Specific Antibody of Serum in the Rat. Korean Journal for Food Science of Animal Resources. 2004;24(3):283-92.
  7. Choi HJ, Sim BY, Joo IH, Yoo SK, Kim DH. Study of Innate Immunity Suppression of Yeonsan Ogye listed on Dong-eui-bo-gam. Journal of Physiology & Pathology in Korean Medicine. 2015;30(4):236-41. https://doi.org/10.15188/kjopp.2016.08.30.4.236
  8. Kim JH, Shin HK. Analysis of Biological Experiment on Immunoactivity of Sipjeondabo-tang. Journal of Physiology & Pathology in Korean Medicine. 2012;26(5):641-9.
  9. Bosca L, Zeini M, Traves PG, Hortelano S. Nitric oxide and cell viability in inflammatory cells: a role for NO in macrophage function and fate. Toxicology. 2005;208(2):249-58. https://doi.org/10.1016/j.tox.2004.11.035
  10. Nathan C. Nitric oxide as a secretory product of mammalian cells. The FASEB journal. 1992;6(12):3051-64. https://doi.org/10.1096/fasebj.6.12.1381691
  11. Turini ME, DuBois RN. Cyclooxygenase-2: a therapeutic target. Annual review of medicine. 2002;53(1):35-57. https://doi.org/10.1146/annurev.med.53.082901.103952
  12. Nathan C. Natural resistance and nitric oxide. Cell. 1995;82(6):873-6. https://doi.org/10.1016/0092-8674(95)90019-5
  13. Fenton MJ, Golenbock DT. LPS-binding proteins and receptors. Journal of leukocyte biology. 1998;64(1):25-32. https://doi.org/10.1002/jlb.64.1.25
  14. Glauser MP. The inflammatory cytokines. Drugs. 1996;52(2):9-17.
  15. Kim JB, Kyung HS, Kang H. Anti-inflammatory Effect of Euphorbiae kansui Radix Extract in Lipopolysaccharide-stimulated Mouse Peritoneal Macrophages. Journal of Physiology & Pathology in Korean Medicine. 2014;28(6):593-600. https://doi.org/10.17208/kjopp.2014.12.28.6.593
  16. Grahames CB, Michel AD, Chessell IP, Humphrey PP. Pharmacological characterization of ATP‐and LPSinduced IL‐$1{\beta}$ release in human monocytes. British journal of pharmacology. 1999;127(8):1915-21. https://doi.org/10.1038/sj.bjp.0702732
  17. Van Snick J. Interleukin-6: an overview. Annual review of immunology. 1990;8(1):253-78. https://doi.org/10.1146/annurev.iy.08.040190.001345
  18. Wink DA, Mitchell JB. Chemical biology of nitric oxide: insights into regulatory, cytotoxic, and cytoprotective mechanisms of nitric oxide. Free Radical Biology and Medicine. 1998;25(4):434-56. https://doi.org/10.1016/S0891-5849(98)00092-6
  19. Kubes P. Inducible nitric oxide synthase: a little bit of good in all of us. Gut. 2000;47(1):6-9. https://doi.org/10.1136/gut.47.1.6
  20. Nakagawa T, Yokozawa T. Direct scavenging of nitric oxide and superoxide by green tea. Food and Chemical Toxicology. 2002;40(12):1745-50. https://doi.org/10.1016/S0278-6915(02)00169-2
  21. McCartney-Francis N, Allen JB, Mizel DE, Albina JE, Xie QW, et al. Suppression of arthritis by an inhibitor of nitric oxide synthase. Journal of Experimental Medicine. 1993;178(2):749-54. https://doi.org/10.1084/jem.178.2.749
  22. Moncada SR, Palmer RM, Higgs E. Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacological reviews. 1991;43(2):109-42.
  23. Kim DH, Park SJ, Jung JY, Kim SC, Byun SH. Anti-inflammatory Effects of the Aqueous Extract of Hwangnyeonhaedok-tang in LPS-activated Macrophage Cells. The Korea Journal of Herbology. 2009;24(4):39-47.
  24. Wang MT, Honn KV, Nie D. Cyclooxygenases, prostanoids, and tumor progression. Cancer and Metastasis Reviews. 2007;26(3-4):525. https://doi.org/10.1007/s10555-007-9096-5
  25. Delgado AV, McManus AT, Chambers JP. Production of tumor necrosis factor-alpha, interleukin 1-beta, interleukin 2, and interleukin 6 by rat leukocyte subpopulations after exposure to substance P. Neuropeptides. 2003;37(6):355-61. https://doi.org/10.1016/j.npep.2003.09.005
  26. Masters SL, Simon A, Aksentijevich I, Kastner DL. Horror autoinflammaticus: the molecular pathophysiology of autoinflammatory disease. Annual review of immunology. 2009;27:621-68. https://doi.org/10.1146/annurev.immunol.25.022106.141627
  27. Cha JH, Kim YS, Lee EM. Effects of Prunellae Spica water extract on immune response in macrophage cells. The Journal of Korean Obstetrics and Gynecology. 2010;23(3):91-100.
  28. Li L, Li H, Qian J, He Y, Zheng J, et al. Structural and immunological activity characterization of a polysaccharide isolated from Meretrix meretrix Linnaeus. Marine drugs. 2015 Dec 29;14(1):6. https://doi.org/10.3390/md14010006
  29. Singh VK, Mehrotra S, Agarwal SS. The paradigm of Th1 and Th2 cytokines. Immunologic research. 1999;20(3):147-61. https://doi.org/10.1007/BF02786470
  30. Church MK, Levi-Schaffer F. The human mast cell. Journal of Allergy and Clinical Immunology. 1997;99(2):155-60. https://doi.org/10.1016/S0091-6749(97)70089-7
  31. Serhan CN, Chiang N, Van Dyke TE. Resolving inflammation: dual anti-inflammatory and pro-resolution lipid mediators. Nature Reviews Immunology. 2008;8(5):349-61. https://doi.org/10.1038/nri2294
  32. Lee JY, Lee YJ, Park WS. Anti-inflammatory effects of fermented Houttuyniae herba water extract on LPS-induced mouse macrophage. The Korea Journal of Herbology. 2010;25(3):27-34.
  33. Zhang Y, Ramos BF, Jakschik BA. from Mast Cells in Immune Complex Peritonitis. Science. 1992;258:1957. https://doi.org/10.1126/science.1470922
  34. Nickoloff BJ, Karabin GD, Barker JN, Griffiths CE, Sarma V, Mitra RS, Elder JT, Kunkel SL, Dixit VM. Cellular localization of interleukin-8 and its inducer, tumor necrosis factor-alpha in psoriasis. The American journal of pathology. 1991;138(1):129.
  35. Walsh LJ, Trinchieri G, Waldorf HA, Whitaker D, Murphy GF. Human dermal mast cells contain and release tumor necrosis factor alpha, which induces endothelial leukocyte adhesion molecule 1. Proceedings of the National Academy of Sciences. 1991;88(10):4220-4. https://doi.org/10.1073/pnas.88.10.4220
  36. Abbas AK, Lichtman AH. Cellular and Molecular Immunology. Bum Moon Sa. Korea. 2004. p. 180-6, 243-74.
  37. Jeong HJ, Chung HS, An HJ, Kim JB, Lee EM, et al. Immune-enhancement effect of the herbal combination Allergina. Clinica chimica acta. 2003;337(1):77-84. https://doi.org/10.1016/j.cccn.2003.07.001

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