The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Antagonistic Assay of Bacillus spp. for Eco-friendly Biological Control of Melon Powdery Mildew

멜론 흰가루병 친환경 생물적 방제를 위한 Bacillus속 균의 길항력 평가

  • Park, Myung Soo (School of Biological Sciences and Institute of Microbiology, Seoul National University) ;
  • Lee, Moon Haeng (Fruit-Vegetable Research Institute, Chungcheongnam-do Agricultural Research and Extension Services) ;
  • Lee, Eun Mo (Fruit-Vegetable Research Institute, Chungcheongnam-do Agricultural Research and Extension Services) ;
  • Yun, Hae-Kuen (Department of Horticulture and Life Science, Yeungnam University) ;
  • Kim, Sung Eok (Sangmyung University) ;
  • Jeon, Nak Beom (Fruit-Vegetable Research Institute, Chungcheongnam-do Agricultural Research and Extension Services)
  • Received : 2017.10.30
  • Accepted : 2018.01.20
  • Published : 2018.03.01
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Abstract

Melon powdery mildew, caused by Podosphaera fusca, is one of the serious diseases of melon plant in Korea. In this study, we evaluated the effect of selected antagonistic bacteria on the inhibition of mycelial growth of various plant pathogens, and control of melon powdery mildew. Based on the 16S rDNA and gyrA gene sequences, the selected antagonistic bacteria, M09, M70, and M99-1, were identified as Bacillus velezensis. These bacteria not only inhibited the mycelial growth of 47~69% in various plant pathogens, but also significantly reduced the incidence of powdery mildew. The three strains selected in this study could be used as potential biological control agents for various plant diseases as well as melon powdery mildew.

Podosphaera fusca에 의한 멜론 흰가루병은 우리나라에서 멜론의 심각한 식물병 중의 하나이다. 본 연구에서는 선발한 길항세균의 다양한 식물병원균에 대한 균사 생육 억제 및 메론 흰가루병 방제에 대한 효과를 평가하였다. 16S rDNA 및 gyrA 유전자의 염기서열을 바탕으로 선발한 길항세균 Bacillus sp. M09, M70 및 M99-1을 동정한 결과 B. velezensis로 동정되었다. B. velezensis M09, M70 및 M99-1는 다양한 식물병원균에 47~69%의 균사생육 억제 효과를 보였을뿐만 아니라 흰가루병균의 발생을 현저히 감소시켰다. 본 연구에서 선발한 3균주는 멜론 흰가루병뿐만 아니라 다양한 식물병에 대한 잠재적인 생물방제제로 활용될 수 있을 것으로 생각되었다.

Keywords

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