The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Isolation of Wild Yeasts from Humus-rich Soil in City Park of Daejeon Metropolitan City, Korea, and Characterization of the Unrecorded Wild Yeasts

대전광역시 도시 자연공원 부엽토와 주변 산림토양들로부터 야생효모의 분리, 동정 및 국내 미기록 야생효모들의 특성

  • Han, Sang-Min (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Lee, Sang-Yeop (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology, Paichai University)
  • 한상민 (배재대학교 바이오.의생명공학과) ;
  • 이상엽 (배재대학교 바이오.의생명공학과) ;
  • 이종수 (배재대학교 바이오.의생명공학과)
  • Received : 2018.02.08
  • Accepted : 2018.02.21
  • Published : 2018.03.01
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Abstract

Totally 91 strains of wild yeasts were isolated from the humus rich soil in the Bogyong city park of Daejeon city, Korea. Majority of the strains belonged to Cryptococcus spp., which included 11 strains of Cryptococcus aureus. Among them, Kwoniella mangroviensis JSS0500, Candida corydalis JSS0501, Candida bombi JSS0503, Candida multigemmis JSS0504, Cryptococcus dimennae JSS0506, Cryptococcus saitoi JSS0507, Cryptococcus victoriae JSS0508, Metschnikowia pulcherrima JSS0502, Papiliotrema aurea JSS0505, Debaryomyces vanrijia JSS0509, Occultifur externus JSS0510, Filobasidium floriforme JSS0511 and Yamadazyma scolyti JSS0512 represented newly recorded yeast strains in Korea, and their microbiological characteristics were investigated. All of these unrecorded yeasts showed oval shape and also formed ascospores and pseudomycelia, except for Kwoniella mangroviensis JSS0500, Candida bombi JSS0503, and Metschnikowia pulcherrima JSS0502. Seven strains including Candida corydalis JSS0501 grew in vitamin-free medium, and 4 of the wild yeasts including Cryptococcus victoriae JSS0508 were halotolerant, i.e., capable of growing in 10% NaCl-containing yeast extract-peptone-dextrose (YPD) broth. Debaryomyces vanrijia JSS0509 was found to be a thermophilic yeast that grew at $37^{\circ}C$.

대전광역시 유성구 복용 자연공원 부엽토와 주변 토양 85점을 채취하여 야생효모들을 분리, 동정한 결과, 야생효모 49종 91균주들이 분리, 동정되었다. 가장 많이 분리된 야생효모는 Cryptococcus속 균이었고 Cryptococcus aureus이 11균주로 가장 많았다. 또한 분리균 중 Kwoniella mangroviensis JSS0500, Candida corydali JSS0501, Candida bombi JSS0503, Candida multigemmis JSS0504, Cryptococcus dimennae JSS0506, Cryptococcus saitoi JSS0507, Cryptococcus victoriae JSS0508, Metschnikowia pulcherrima JSS0502, Papiliotrema aurea JSS0505, Debaryomyces vanrijia JSS0509, Occultifur externus JSS0510, Filobasidium floriforme JSS0511과 Yamadazyma scolyti JSS0512 등 13균주들이 국내 미기록 효모 균주들로 선별되었고 이들 야생효모들은 구형으로 출아법으로 영양증식하였다. Candida corydali JSS0501 등 7균주들이 vitamin을 함유하지 않은 YPD 배지에서도 잘 생육하였고 Cryptococcus victoriae JSS0508 등은 10% NaCl를 함유한 YPD 배지에서 생육하는 호염성 효모들이었다.

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References

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