Anatomy and Cell Biology

  • 제51권1호
  • /
  • Pages.52-61
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  • 2018
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  • 2093-3665(pISSN)
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  • 2093-3673(eISSN)
대한해부학회 (Korean Association of Anatomists)
권호 표지
DOI QR코드

DOI QR Code

Ameliorative effect of vitamin B12 on seminiferous epithelium of cimetidine-treated rats: a histopathological, immunohistochemical and ultrastructural study

  • Elsaed, Wael M. (Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University) ;
  • Bedeer, Raouf Fekry (Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University) ;
  • Eladl, Mohamed Ahmed (Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University)
  • 투고 : 2017.11.27
  • 심사 : 2018.01.10
  • 발행 : 2018.03.31
⟨ 이전 논문 다음 논문 ⟩

초록

Cimetidine is an H2 receptor antagonist that has an antiandrogenic effect. It intervenes with the conversion of testosterone into estrogen in the Sertoli cells with accompanying testicular structural changes. In the present study, the microscopic and the ultrastructural changes induced by cimetidine and the effect of vitamin B12 as a protective agent on rat testes were studied. Immunoexpression of estrogen receptor ${\beta}$ ($ER{\beta}$) in testes was evaluated. Twenty-four adult male rats were divided into four groups: control, cimetidine-treated, vitamin B12 treated, and combined cimetidine and vitamin B12 treated. The experimental rats were administered with cimetidine and/or vitamin B12 for 52 days. Group II rats showed marked atrophy of the seminiferous tubules with a significant increase in tubular diameter and decrease in the tubular luminal and epithelial areas. Ultrastructure of this group showed irregular Sertoli cells with basal cytoplasmic vacuolation and significantly thickened basement membrane. $ER{\beta}$ immunoexpression was similar to controls. Group III rats showed near normal seminiferous tubular structures with minimal cellular alterations and the immunoreactivity of the testicular sections was very close to normal. However, group IV rats showed markedly immunopositive detached cells, spermatids, and primary spermatocytes. Cimetidine interferes with the control of spermatogenesis as evidenced by microscopic and ultrastructural studies and affection of $ER{\beta}$ receptors and vitamin B12 has a protective action against this harmful effect.

키워드

참고문헌

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피인용 문헌

  1. Effect of Nandrolone decanoate induced-oxidative stress on rat testes, prostate, and seminal vesicle: Biochemical, morphometric and histopathological studies vol.28, pp.1, 2018, https://doi.org/10.1016/j.sjbs.2020.09.039