Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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miRNA-103a-3p Promotes Human Gastric Cancer Cell Proliferation by Targeting and Suppressing ATF7 in vitro

  • Hu, Xiaoyi (Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University) ;
  • Miao, Jiyu (Key Laboratory of Environment and Genes Related to Diseases, College of Medicine, Xi'an Jiaotong University) ;
  • Zhang, Min (College of Life Science, Yanan University) ;
  • Wang, Xiaofei (Key Laboratory of Environment and Genes Related to Diseases, College of Medicine, Xi'an Jiaotong University) ;
  • Wang, Zhenzhen (Key Laboratory of Environment and Genes Related to Diseases, College of Medicine, Xi'an Jiaotong University) ;
  • Han, Jia (Key Laboratory of Environment and Genes Related to Diseases, College of Medicine, Xi'an Jiaotong University) ;
  • Tong, Dongdong (Key Laboratory of Environment and Genes Related to Diseases, College of Medicine, Xi'an Jiaotong University) ;
  • Huang, Chen (Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University)
  • Received : 2017.05.15
  • Accepted : 2018.03.15
  • Published : 2018.05.31
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Abstract

Studies have revealed that miR-103a-3p contributes to tumor growth in several human cancers, and high miR-103a-3p expression is associated with poor prognosis in advanced gastric cancer (GC) patients. Moreover, bioinformatics analysis has shown that miR-103a-3p is upregulated in The Cancer Genome Atlas (TCGA) stomach cancer cohort. These results suggest that miR-103a-3p may function as an oncogene in GC. The present study aimed to investigate the role of miR-103a-3p in human GC. miR-103a-3p expression levels were increased in 33 clinical GC specimens compared with adjacent nontumor stomach tissues. Gain- and loss-of-function studies were performed to identify the correlation between miR-103a-3p and tumorigenesis in human GC. Inhibiting miR-103a-3p suppressed GC cell proliferation and blocked the S-G2/M transition in MKN-45/SGC-7901 cells, whereas miR-103a-3p overexpression improved GC cell proliferation and promoted the S-G2/M transition in vitro. Bioinformatics and dual-luciferase reporter assays confirmed that ATF7 is a direct target of miR-103a-3p. Analysis of the TCGA stomach cancer cohort further revealed that miR-103a-3p expression was inversely correlated with ATF7 expression. Notably, silencing ATF7 showed similar cellular and molecular effects as miR-103a-3p overexpression, namely, increased GC cell proliferation, improved CDK2 expression and decreased P27 expression. ATF7 overexpression eliminated the effects of miR-103a-3p expression. These findings indicate that miR-103a-3p promotes the proliferation of GC cell by targeting and suppressing ATF7 in vitro.

Keywords

References

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