생명과학회지 (Journal of Life Science)

  • 제28권5호
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  • Pages.540-546
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  • 2018
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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그라비올라 잎 추출물의 이화학적 특성 및 항산화 활성

Antioxidative Effects and Chemical Characteristics of Annona muricata Leaf Extracts

  • 투고 : 2018.02.01
  • 심사 : 2018.03.05
  • 발행 : 2018.05.30
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초록

그라비올라(Annona muricata) 잎으로부터 정제수, 에탄올, 메탄올로 추출하여 수율, 총 페놀성 화합물과 이에 속하는 flavonoid 함량, 미네랄 함량을 측정하고 DPPH free radical scavenging 활성, Cu/Fe 환원력, 간 조직의 microsome 생체막의 지질과산화 억제능 및 linoleic acid를 이용한 지질과산화, ${\beta}$-Carotene 탈색화 정도를 비교검토하였다. 그라비올라 잎의 수율은 수용성 추출물이 가장 높았으며(1.76%), 총 페놀성 화합물 함량은 그라비올라의 메탄올 추출물에서 가장높았으나 페놀성 화합물에 속하는 폴리페놀 함량은 에탄올 추출물에서 가장 높은 결과를 나타내었고, 미네랄 함량은 Ca, K, Mg, Na, Mn의 순으로 높게 측정되었다. DPPH를 이용한 radical 소거능을 측정한 결과, 그라비올라 잎의 에탄올과 메탄올 추출물에서 대조구인 BHT 0.05%와 가장 근접한 활성을 나타내었다. Fe와 Cu의 환원력은 모든 군에서 농도 의존적으로 증가하였고, 그 중 에탄올과 메탄올 추출물에서 Cu/Fe 환원력 활성이 매우 강하였다. 그러나 간 조직을 이용하여 측정하는 지질과산화 억제활성과 TBA법으로 지질과산화를 측정한 결과, 그라비올라 잎의 에탄올 추출물에서 지질과산화 억제능을 보였다. ${\beta}$-Carotene bleaching에서 높은 lipid peroxyl 라디칼 소거 활성이 있음을 확인할 수 있었다. 따라서, 그라비올라 잎은 기능성 식품의 개발을 위한 천연 항산화제 소재로서 활용 될 수 있을 것으로 판단되어진다.

Annona muricata, generally known as soursop, graviola, or sirsak, is native to the warmest tropical areas of North and South America and is now widely distributed throughout tropical and subtropical parts of the world, including India and Nigeria. This study tested the contents of polyphenolic compounds, flavonoids, and minerals, as well as the antioxidative effects of DPPH radical-scavenging activity, Fe/Cu-reducing power, linoleic-acid peroxidation using thiobarbituric-acid (TBA) methods and peroxidation of rat-hepatocyte microsomes, and ${\beta}$-carotene bleaching assay. These were tested with in-vitro experimental models using water, ethanol, and methanol extracts of the Annona muricata leaf (AMl). Water extracts of AMl showed the highest extraction yield (1.76%). The total polyphenol-compound concentration was the highest in the methanol extract of AMl. However, the flavonoids concentration was the highest in the ethanol extracts of AMl. AMlMl major minerals were Ca, K, and Mg. In DPPH radical-scavenging activity, the contents exhibited a strong scavenging effect on the ethanol and methanol extracts of AMl. Additionally, the Fe/Cu-reducing power was strong in ethanol and methanol extracts of AMl. $Fe^{2+}$/ascorbate-induced linoleic-acid peroxidation using TBA methods and auto-oxidation of rat-hepatic microsomes showed strong antioxidative activities in ethanol extracts of AMl. ${\beta}$-Carotene bleaching was also highest in the ethanol extracts of AMl. These results may provide the basic data to understand the chemical characteristics and antioxidative effects of Annona muricata leaf extract for the development of functional foods.

키워드

참고문헌

  1. A.O.A.C. 1975. Official methods of analysis. 12th ed., Association of official analytical chemists. Washington, D.C., U.S.A.
  2. Saiga, A., Tanabe, S. and Nishimura, T. 2003. Antioxidant activity of peptides obtained from porcine myofibrillar proteins by protease treatment. J. Agric. Food Chem. 51, 3661-3667. https://doi.org/10.1021/jf021156g
  3. Baskar, R., Rajeswari, V. and Kumar, T. S. 2007. In vitro antioxidant studies in leaves of Annona species. Indian J. Exp. Biol. 45, 480-485.
  4. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1204.
  5. Cha, J. Y. and Cho, Y. S. 1999. Effect of potato polyphenolics on lipid peroxidation in rats. J. Kor. Soc. Food Sci. Nutr. 28, 1131-1136.
  6. Cha, J. Y., Ahn, H. Y., Eom, K. E., Park, B. K., Jun, B. S. and Cho, Y. S. 2009. Antioxidative activity of Aralia elata shoot and leaf extracts. J. Life Sci. 19, 652-658. https://doi.org/10.5352/JLS.2009.19.5.652
  7. Choe, S. Y. and Yang, K. H. 1982. Toxicological studies of antioxidants, butylated hydroxytoluene (BHT) and butylated hydroxyanisol (BHA). Kor. J. Food Sci. Technol. 14, 283-288.
  8. Choi, J. I., Kim, Y. J., Kim, J. H., Song, B. H., Yoon, Y. H., Byun, M. W., Kwon, J. H., Chun, S. S. and Lee, J. W. 2009. Antioxidant activities of the extract fractions from Suaeda japonica. J. Kor. Soc. Food Sci. Nutr. 38, 131-135. https://doi.org/10.3746/jkfn.2009.38.2.131
  9. Duncan, D. B. 1959. Multiple range and multiple F test. Biometrics 1, 1-42.
  10. Jia, Z., Tang, M. and Wu, J. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64, 555-559. https://doi.org/10.1016/S0308-8146(98)00102-2
  11. Kim, E. Y., Baik, I. H., Kim, J. H., Kim, S. R. and Rhyu, M. R. 2004. Screening of the antioxidant activity of some medicinal plants. Kor. J. Food Sci. Technol. 36, 333-338.
  12. Kim, J. S., Lee, Y. J., Yang, J. F., Sa, Y. J., Kim, M. O., Park, J. H., Park, D. S., Yu, C. Y. and Kim, M. J. 2013. Biological activity of Sorghum bicolor M. cv. Bulgeunjangmoksusu extracts. Kor. J. Plant Res. 26, 111-118. https://doi.org/10.7732/kjpr.2013.26.1.111
  13. Kim, M. J., Cho, S. Y., Lee, M. K. and Shin, K. H. 2004. Effects of Aralia elata water extracts on activities of hepatic oxygen free radical generating and scavenging enzymes in streptozotocin-induced diabetic rats. J. Kor. Soc. Food Sci. Nutr. 33, 653-658. https://doi.org/10.3746/jkfn.2004.33.4.653
  14. Kim, M. J., Chu, W. M. and Park, E. J. 2012. Antioxidant and antigenotoxic effects of shiitake mushrooms affected by different drying methods. J. Kor. Soc Food Sci. Nutr. 41, 1041-1048. https://doi.org/10.3746/jkfn.2012.41.8.1041
  15. Lee, H. J., Lee, B. J., Lee, D. S. and Seo, Y. W. 2003. DPPH radical scavenging effect and in vitro lipid peroxidation inhibition by Portulaca oleracea. Kor. J. Biotechnol. Bioeng. 3, 165-169.
  16. Luthringer, C., Rayssiguier, Y., Gueux, E. and Berthelot, A. 1988. Effect of moderate magnesium deficiency on serum lipids, blood pressure and cardiovascular reactivity in normotensive rats. Br. J. Nutr. 59, 243-250. https://doi.org/10.1079/BJN19880031
  17. Matsushige, A., Kotake, Y., Matsunami, K., Otsuka, H., Ohta, S. and Takeda, Y. 2012. Annonamine, a new aporphine alkaloid from the leaves of Annona muricata. Chem. Pharm. Bull. 60, 257-259. https://doi.org/10.1248/cpb.60.257
  18. Mattaus, B. 2002. Antionidant activity of extracts obtained from residues of different oilseeds. J. Agric Food Chem. 50, 3444-3452. https://doi.org/10.1021/jf011440s
  19. McLaughlin, J. L. 2008. Paw paw and cancer: Annonaceous acetogenins from discovery to commercial products. J. Nat. Prod. 71, 1311-1321. https://doi.org/10.1021/np800191t
  20. Moghadamtoushi, S. Z., Fadaeinasab, M., Nikzad, S., Mohan, G., Ali, H. M. and Kadir, H. A. 2015. Annona muricata (Annonaceae): a review of its traditional uses, isolated acetogenins and biological activities. Int. J. Mol. Sci. 16, 15625-15658. https://doi.org/10.3390/ijms160715625
  21. Swain, T. and Hillis, W. E. 1959. Phenolic constituents of Prunus domestica. I.-The quantitative analysis of phenolic constituents. J. Sci. Food Agric. 10, 83-88.
  22. Wong, S. F., Holliwell, B., Richimond, R. and Skowroneck, W. R. 1981. The role of superoxide and hydroxyl radical in the degradation of hyaluronic acid induced by metal ions and by ascorbic acid. J. Inorganic Biochem. 14, 127-134. https://doi.org/10.1016/S0162-0134(00)80033-1
  23. Yeo, J. S., Chun, S. S. and Choi, J. H. 2014. Antioxidant activities of solvent extracts from Rosa multiflora. J. Life Sci. 24, 1217-1223. https://doi.org/10.5352/JLS.2014.24.11.1217
  24. Zhu, Q. V., Hackman, R. M., Jodilensunsa, X. X., Holt, R. R. and Keen, C. L. 2002. Antioxidative activities of Oolong tea. J. Agric. Food Chem. 50, 6229-6234.