Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Anti-wrinkle and Whitening Effects of Essential Oil from Abies koreana

Abies koreana 유래 정유의 항주름 및 미백 효과

  • Song, Byeong-Wook (Department of Convergence Science, College of Medicine, Catholic Kwandong University) ;
  • Song, Min-Jeong (Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University) ;
  • Park, Mi-Jin (Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science) ;
  • Choi, Don-Ha (Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science) ;
  • Lee, Sung-Suk (Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science) ;
  • Kim, Myungkil (Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science) ;
  • Hwang, Ki-Chul (Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University) ;
  • Kim, Il-Kwon (Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University)
  • 송병욱 (가톨릭관동대학교 의과대학 융합과학교실) ;
  • 송민정 (가톨릭관동대학교 의과대학 바이오융합연구원) ;
  • 박미진 (국립산림과학원 임산공학부 화학미생물과) ;
  • 최돈하 (국립산림과학원 임산공학부 화학미생물과) ;
  • 이성숙 (국립산림과학원 임산공학부 화학미생물과) ;
  • 김명길 (국립산림과학원 임산공학부 화학미생물과) ;
  • 황기철 (가톨릭관동대학교 의과대학 바이오융합연구원) ;
  • 김일권 (가톨릭관동대학교 의과대학 바이오융합연구원)
  • Received : 2018.01.03
  • Accepted : 2018.02.23
  • Published : 2018.05.30
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Abstract

The essential oil from Abies koreana E.H. Wilson had been developed, however, its efficacy has not yet been studied especially in terms of skin care research. The aim of this study is to investigate the effects of Abies koreana extracts (AKE) on melanogenesis and wrinkle formation in B16F10 melanoma cells (B16F10) and human dermal fibroblast cell line (HDF). The essential oil was extracted by hydrodistillation method and purified by anhydrous sodium sulfate. At a concentration of $10^{-5}$-fold, viability in these cells had been defined by cytotoxicity assays. Anti-melanogenic effects on B16F10 were evaluated using tyrosinase inhibition assay, and real-time PCR for verifying gene expression of tyrosinase, tyrosinase related protein-1 and -2 (TRP-1 and -2). AKEs reduced about 5-fold of tyrosinase inhibitory activity compared to ${\alpha}$-melanocyte-stimulating hormone (${\alpha}$-MSH)-induced group and about 30% reduction compared to Arbutin induced group. The mRNA levels of three melanin-related factors were increased, separately. To investigate the effects of anti-wrinkle, procollagen type I c peptide synthesis assay (PIP) and Western blot were performed. At AKE-treated group, PIP was up-regulated and the expression of collagen type 1 and matrix metalloproteinase (MMP)-1 were improved. Furthermore, AKE presented anti-wrinkle effects by increasing UVB-inhibited collagen type 1 expression, and reducing UVB-induced MMP-1 production at $60mJ/cm^2$ of UVB radiation. Therefore, Abies koreana extracts has potentials as a safe and an effective skin ingredient for whitening and anti-wrinkle.

구상나무로부터 추출된 정유가 개발되어 있지만, 피부와 연관된 연구 관점에서 효능이 아직까지 밝혀지지 않았다. 본 연구의 목적은 B16F10 흑색종 세포(B16F10)와 인간 피부아세포주(HDF)에서 멜라닌과 주름 형성 상의 구상나무 추출물 효과를 알아보는데 있다. 정유는 수첨 증류법으로 추출하였고 무수 황산나트륨으로 정제 하였다. $10^{-5}$배의 정유농도에서, 두 세포의 생존능은 세포독성 평가에 의해 확인 되었다. B18F10의 항멜라닌 효과는 티로시나아제 억제 분석과 티로시나아제, 티로시나아제 연관 단백질 -1과 -2(TRP-1, TRP-2)의 유전자 발현 검증을 통해 이루어졌다. 정유는 알파-멜라닌세포 자극 호르몬 유도 그룹과 비교하여 티로시나아제 억제 활성이 5배, 아르부틴 유도 그룹과 비교하였을 때 30% 정도 감소되었다. 3가지 멜라닌 유래 인자들의 mRNA 수준도 각각 증가하였다. 항주름 효과를 분석하기 위해, 프로콜라겐 타입 I c 펩티드 합성(PIP) 분석과 웨스턴 블록을 수행하였다. 정유를 처리한 그룹에서 PIP가 증가되었고 콜라겐 타입 1과 기질 금속 단백질 분해 효소가 개선 되었다. 또한 정유는 $60mJ/cm^2$의 자외선 조건에서, 자외선-억제 콜라겐 타입 1의 증가와 기질 금속 단백질 분해 효소 생성 억제를 통해 항주름 효과를 보여줬다. 따라서 구상나무 추출물은 미백과 항주름을 위한 안전하고 효과적인 피부 제재로서 가능성을 가지고 있음을 암시한다.

Keywords

References

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