DOI QR코드

DOI QR Code

Development of Single-layer Glucose Sensor Using GDH-FAD (Glucose Dehydrogenase Flavin Adenine Dinucleotide)

  • Kye, Ji-Won (REDICARE CO., LTD) ;
  • Lee, Young-Tae (Dept. of Bio-ICT Engineering, Graduate School, Andong National Unversity)
  • 투고 : 2018.05.19
  • 심사 : 2018.05.30
  • 발행 : 2018.05.31

초록

We developed a glucose sensor using glucose dehydrogenase flavin adenine dinucleotide (GDH-FAD). The structure of the three-layer glucose sensor was simplified, in which a single-layer design was used to lower the unit cost, and GDH-FAD was used to increase the measurement reliability. GDH-FAD has less impact on the 20 interfering substances that affect blood glucose measurement, such as galactose and maltose compared to glucose oxidase (GOD), and is not affected by the oxygen saturation; therefore, it is possible to measure both arterial or venous blood and thus less susceptibility to hematocrit. In this study, we developed a single-layer glucose sensor strip with low hematocrit effect using the GDH-FAD enzyme, and measured and evaluated the performance.

키워드

참고문헌

  1. Diabetes Fact Sheet in Korea 2016, Korean Diabetes Association, 2016.
  2. Diabetes Fact Sheet in Korea 2018, Korean Diabetes Association, 2018.
  3. Y. T. Lee, and M. S. Kwon, "Development of single-layer-structured glucose sensor", Journal of the Korea Sensors Society, Vol. 24(2), pp.83-87, 2015.
  4. Y. T. Lee, and S. R. Lee, "Development of the disposable glucose sensor using Cu/Ni/Au electrode", Journal of the Korea Sensors Society, Vol. 15(5), pp.352-356, 2006.
  5. H. Bilen, A. Kilicaslan, G. Akcay, and F. Albayrak, "Performance of glucose dehydrogenase(GDH) based and glucose oxidase(GOX) based blood glucose meter systems", Journal of Medical Engineering and Technology, Vol. 31(2), pp.152-156, 2007. https://doi.org/10.1080/03091900600861590
  6. Joseph Wang, "Electrochemical glucose biosensors", Chemical Reviews, Vol.108(2), pp814-825, 2008. https://doi.org/10.1021/cr068123a
  7. J. R. Mor, and R. Guarnaccia, "Assay of glucose using an electrochemical enzymatic sensor", Analytical Biochemistry, Vol. 79, pp.319-328, 1977. https://doi.org/10.1016/0003-2697(77)90407-9
  8. Z. Tang, J. H. Lee, R. F. Louie, "Effects of different hematocrit levels on glucose measurements with handheld meters for point-of care testing", Arch Pathol Lab Med, Vol.124, pp.1135-1140, 2000.