Korean Circulation Journal

The Korean Society of Cardiology (대한심장학회)
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β-arrestin2 Affects Cardiac Progenitor Cell Survival through Cell Mobility and Tube Formation in Severe Hypoxia

  • Seo, Seul-ki (Department of Internal Medicine, Chungbuk National University College of Medicine) ;
  • Kim, Nari (Department of Internal Medicine, Chungbuk National University College of Medicine) ;
  • Lee, Ju-Hee (Chungbuk Regional Cardiocerebrovascular Center, Chungbuk National University Hospital) ;
  • Kim, Sang Min (Chungbuk Regional Cardiocerebrovascular Center, Chungbuk National University Hospital) ;
  • Lee, Sang Yeub (Department of Internal Medicine, Chungbuk National University College of Medicine) ;
  • Bae, Jang-Whan (Department of Internal Medicine, Chungbuk National University College of Medicine) ;
  • Hwang, Kyung-Kuk (Department of Internal Medicine, Chungbuk National University College of Medicine) ;
  • Kim, Dong-Woon (Department of Internal Medicine, Chungbuk National University College of Medicine) ;
  • Koch, Walter J. (Center for Translational Medicine, Department of Pharmacology, Temple University Lewis Katz School of Medicine) ;
  • Cho, Myeong-Chan (Department of Internal Medicine, Chungbuk National University College of Medicine)
  • Received : 2017.06.06
  • Accepted : 2018.01.17
  • Published : 2018.04.06
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Abstract

Background and Objectives: ${\beta}$-arrestin2 (${\beta}$-arr2) basically regulates multiple signaling pathways in mammalian cells by desensitization and internalization of G-protein coupled receptors (GPCRs). We investigated impacts of ${\beta}$-arr2 on survival, mobility, and tube formation of cardiac progenitor cells (CPCs) obtained from wild-type (WT) mouse (CPC-WT), and ${\beta}$-arr2 knock-out (KO) mouse (CPC-KO) cultured in presence or absence of serum and oxygen as non-canonical roles in GPCR system. Methods: CPCs were cultured in Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12-based media containing fetal bovine serum and growth factors. Survival of 2 types of CPCs in hypoxia and/or serum deprivation was measured by fluorescence-activated cell sorting. Wound healing ability, and tube formation ability on Matrigel of 2 kinds of CPCs were compared in normoxic and hypoxic cultures. Protein expression related to survival and mobility were measured with the Western blot for each culture conditions. Result: CPC-KO showed significantly worse mobility in the wound healing assay and in tube formation on Matrigel especially in hypoxic culture than did the CPC-WT. Also, CPC-KO showed significantly higher apoptosis fraction in both normoxic and hypoxic cultures than did the CPC-WT. Expression of proteins associated with cell survival and mobility, e.g., protein kinase B (Akt), ${\beta}$-catenin, and glycogen synthase kinase-$3{\beta}$ (GSK-$3{\beta}$) was significantly worse in CPC-KO. Conclusions: The CPC-KO had significantly worse cell mobility, tube formation ability, and survival than the CPC-WT, especially in the hypoxic cultures. Apparently, ${\beta}$-arr2 is important on CPC survival by means of mobility and tube formation in myocardial ischemia.

Keywords

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