The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Comparison of Anti-inflammatory effects between Artemisia capillaris and Artemisia iwayomogi by extraction solvents

인진호(茵蔯蒿)와 한인진(韓茵蔯)의 추출용매별 항염증 효능 비교

  • Noh, Dongjin (Department of Life and Nanopharmaceutical Science, Graduate School, Kyung Hee University) ;
  • Choi, Jin Gyu (BK21 PLUS Integrated Education and Research Center for Nature-inspired Drug Development Targeting Healthy Aging, Kyung Hee University) ;
  • Hong, Soon-Sun (Department of Biomedical Sciences, College of Medicine, Inha University) ;
  • Oh, Myung Sook (Department of Life and Nanopharmaceutical Science, Graduate School, Kyung Hee University)
  • 노동진 (경희대학교 일반대학원 나노의약생명과학과) ;
  • 최진규 (경희대학교 BK21 플러스 고령사회 대비 천연물-바이오 융합 신약개발 창의인재양성 사업단) ;
  • 홍순선 (인하대학교 의과대학 의생명학과) ;
  • 오명숙 (경희대학교 일반대학원 나노의약생명과학과)
  • Received : 2018.04.11
  • Accepted : 2018.05.25
  • Published : 2018.05.30
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Abstract

Objectives : Artemisia capillaris Thunberg (AC) and Artemisia iwayomogi Kitamura (AI) have been used without distinguishment since ancient times due to similar appearance. In this study, we compared the inhibitory effects of AC and AI on the expression of inflammatory cytokines induced by lipopolysaccharide (LPS) in murine macrophages. Methods : AC and AI were extracted by reflux with distilled water (DW) and 70% ethanol (EtOH). We investigated the inhibitory effects of AC and AI on the expression of nitric oxide (NO), inducible NO synthase (iNOS) and tumor necrosis $factor-{\alpha}$($TNF-{\alpha}$) induced by LPS in macrophages. Results : Firstly, yield of the samples was higher in order of Artemisia iwayomogi DW Extract (AID), Artemisia iwayomogi 70% EtOH Extract (AIE), Artemisia capillaris DW Extract (ACD) and Artemisia capillaris 70% EtOH Extract (ACE). All of the samples were not toxic in macrophages. The inhibitory effect of the samples on LPS-induced NO expression was stronger in the order of AIE, ACE, AID and ACD. The inhibitory effect of the samples on LPS-induced inducible iNOS expression was stronger in the order of AIE, ACE and AID. Effect of ACD was same with that of AID. In addition, inhibitory effect of the samples on LPS induced $TNF-{\alpha}$expression wes stronger in the order of AIE, ACE, AID and ACD. Conclusion: These results showed that AI would be more effective than AC and 70% EtOH would be more effective than DW as an extraction solvent in inflammatory diseases.

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