Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Protective Effects of Flavonoids from the Boehmeria quelpaertense against H2O2-Induced Cytotoxicity in H9c2 Cardiomyoblast Cells

H9c2 심근세포에서 제주모시풀(Boehmeria quelpaertense)로부터 분리된 flavonoids의 H2O2로 유도된 독성 보호 효과

  • Woo, Kyeong-Wan (Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine) ;
  • Sim, Mi-Ok (Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine) ;
  • Bak, Ho (Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine) ;
  • Jung, Ho Kyung (Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine) ;
  • An, Byeongkwan (Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine) ;
  • Ham, Seong-Ho (Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine) ;
  • Park, Jong Hyuk (Luco Co. Ltd) ;
  • Cho, Hyun-Woo (Traditional Korean Medicines Research Team, Naional Development Institute of Korea Medicine)
  • 우경완 (한약진흥재단 한약자원본부 한약재연구팀) ;
  • 심미옥 (한약진흥재단 한약자원본부 한약재연구팀) ;
  • 박호 (한약진흥재단 한약자원본부 한약재연구팀) ;
  • 정호경 (한약진흥재단 한약자원본부 한약재연구팀) ;
  • 안병관 (한약진흥재단 한약자원본부 한약재연구팀) ;
  • 함성호 (한약진흥재단 한약자원본부 한약재연구팀) ;
  • 박종혁 ((주)루코) ;
  • 조현우 (한약진흥재단 한약자원본부 한약재연구팀)
  • Received : 2017.08.07
  • Accepted : 2017.10.16
  • Published : 2018.02.28
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Abstract

As a part of an infrastructure project on medicinal herb-based remedies, we conducted a phytohemical investigation of the 100% MeOH extract from the aerial part of Boehmeria quelpaertense; our findings resulted in the isolation of flavonoids (1-2), isoquercitrin (1) and hyperoside (2). The identification and structural elucidation of these compounds were based on $^1H$-, $^{13}C-NMR$, and LC ESI IT-TOF MS data. All the compounds isolated from this plant were reported for the first time. In this study, we examined the antioxidant activity of the 1 and 2 on the hydrogen peroxide ($H_2O_2$)-induced oxidative stress in a Rat Cardiomyoblast cell line (H9c2). The pretreatment of the flavonoids showed that it protects against $H_2O_2$-mediated cell death in the H9c2 cell line. Also, it decreases the intracellular reactive oxygen species (ROS) levels by the flavonoids in the $H_2O_2$-treated H9c2 cell line. These results showed that the 1 and 2 are a source of antioxidants. As a result, they might be helpful in preventing the progress of various oxidative stress mediated diseases, including myocardial infarction.

제주모시풀에서 각종 column chromatography법을 이용하여 2종의 flavonoid를 분리하여, $^1H$-, $^{13}C-NMR$, LC ESI-IT-TOF MS를 통해 구조동정 할 수 있었다. 분리된 물질이 심장세포에서 높은 항산화 활성을 가지고 있으며, 활성 산소종의 작용기전 연구 및 심근경색 질환의 예방과 치료에 효과적으로 사용할 수 있는 기초자료가 될 것으로 사료된다.

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