Restorative Dentistry and Endodontics

  • 제43권1호
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  • Pages.11.1-11.10
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  • 2018
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  • 2234-7658(pISSN)
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  • 2234-7666(eISSN)
대한치과보존학회 (The Korean Academy of Conservative Dentistry)
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Mineral content analysis of root canal dentin using laser-induced breakdown spectroscopy

  • Eren, Selen Kucukkaya (Department of Endodontics, Faculty of Dentistry, Hacettepe University) ;
  • Uzunoglu, Emel (Department of Endodontics, Faculty of Dentistry, Hacettepe University) ;
  • Sezer, Banu (Department of Food Engineering, Faculty of Engineering, Hacettepe University) ;
  • Yilmaz, Zeliha (Department of Endodontics, Faculty of Dentistry, Hacettepe University) ;
  • Boyaci, Ismail Hakki (Department of Food Engineering, Faculty of Engineering, Hacettepe University)
  • 투고 : 2017.10.16
  • 심사 : 2018.01.18
  • 발행 : 2018.02.02
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초록

Objectives: This study aimed to introduce the use of laser-induced breakdown spectroscopy (LIBS) for evaluation of the mineral content of root canal dentin, and to assess whether a correlation exists between LIBS and scanning electron microscopy/energy dispersive spectroscopy (SEM/EDS) methods by comparing the effects of irrigation solutions on the mineral content change of root canal dentin. Materials and Methods: Forty teeth with a single root canal were decoronated and longitudinally sectioned to expose the canals. The root halves were divided into 4 groups (n = 10) according to the solution applied: group NaOCl, 5.25% sodium hypochlorite (NaOCl) for 1 hour; group EDTA, 17% ethylenediaminetetraacetic acid (EDTA) for 2 minutes; group NaOCl+EDTA, 5.25% NaOCl for 1 hour and 17% EDTA for 2 minutes; a control group. Each root half belonging to the same root was evaluated for mineral content with either LIBS or SEM/EDS methods. The data were analyzed statistically. Results: In groups NaOCl and NaOCl+EDTA, the calcium (Ca)/phosphorus (P) ratio decreased while the sodium (Na) level increased compared with the other groups (p < 0.05). The magnesium (Mg) level changes were not significant among the groups. A significant positive correlation was found between the results of LIBS and SEM/EDS analyses (r = 0.84, p < 0.001). Conclusions: Treatment with NaOCl for 1 hour altered the mineral content of dentin, while EDTA application for 2 minutes had no effect on the elemental composition. The LIBS method proved to be reliable while providing data for the elemental composition of root canal dentin.

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참고문헌

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