Restorative Dentistry and Endodontics

  • 제43권1호
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  • Pages.1.1-1.8
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  • 2018
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  • 2234-7658(pISSN)
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  • 2234-7666(eISSN)
대한치과보존학회 (The Korean Academy of Conservative Dentistry)
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Root canal irrigants influence the hydrophobicity and adherence of Staphylococcus epidermidis to root canal dentin: an in vitro study

  • Nagendrababu, Venkateshbabu (Division of Clinical Dentistry, School of Dentistry, International Medical University) ;
  • Sultan, Omer Sheriff (Division of Clinical Dentistry, School of Dentistry, International Medical University) ;
  • Kannathasan, Sreedharan (School of Medicine, International Medical University) ;
  • Patel, Amir Shahreza (School of Medicine, International Medical University) ;
  • Chitra, Ebenezer (Division of Applied Biomedical Science and Biotechnology, School of Health Sciences, International Medical University) ;
  • Neelakantan, Prasanna (Discipline of Endodontology, Faculty of Dentistry, The University of Hong Kong) ;
  • Davamani, Fabian (Division of Applied Biomedical Science and Biotechnology, School of Health Sciences, International Medical University)
  • 투고 : 2017.07.31
  • 심사 : 2017.11.06
  • 발행 : 2018.02.02
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초록

Objectives: To determine the effect of root canal irrigants on the hydrophobicity and adherence of Staphylococcus epidermidis (S. epidermidis) to root canal dentin in vitro. Materials and Methods: Root dentin blocks (n = 60) were randomly divided into 4 groups based on the irrigation regimen: group 1, saline; group 2, 5.25% sodium hypochlorite (NaOCl); group 3, 5.25% NaOCl followed by 17% ethylenediaminetetraacetic acid (EDTA); group 4, same as group 3 followed by 2% chlorhexidine (CHX). The hydrophobicity of S. epidermidis to root dentin was calculated by cell surface hydrophobicity while the adherence was observed by fluorescence microscopy, and bacteria were quantified using ImageJ software (National Institutes of Health). Statistical analysis of the data was done using Kruskal-Wallis test and Mann-Whitney U test (p = 0.05). Results: The hydrophobicity and adherence of S. epidermidis to dentin were significantly increased after irrigating with group 3 (NaOCl-EDTA) (p < 0.05), whereas in group 4 (NaOCl-EDTA-CHX) both hydrophobicity and adherence were significantly reduced (p < 0.05). Conclusions: The adherence of S. epidermidis to dentin was influenced differently by root canal irrigants. Final irrigation with CHX reduces the bacterial adherence and may impact biofilm formation.

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참고문헌

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