Clinical and Experimental Reproductive Medicine

  • 제45권4호
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  • Pages.163-169
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  • 2018
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
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Protective effects of saffron against zearalenone-induced alterations in reproductive hormones in female mice (Mus musculus)

  • Ahmad, Bashir (Laboratory of Endocrinology, Department of Bioscience, Barkatullah University) ;
  • Shrivastava, Vinoy K. (Laboratory of Endocrinology, Department of Bioscience, Barkatullah University) ;
  • Saleh, Ramadan (Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Sohag University) ;
  • Henkel, Ralf (Department of Medical Bioscience, University of the Western Cape) ;
  • Agarwal, Ashok (American Center for Reproductive Medicine, Urological Institute, Cleveland Clinic)
  • 투고 : 2018.05.06
  • 심사 : 2018.10.16
  • 발행 : 2018.12.31
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초록

Objective: Zearalenone (ZEA) is a mycotoxin with potent estrogenic effects. Saffron is an herbal product that has antioxidant activities. The objective of this study was to investigate the protective role of saffron against reproductive toxicity induced by ZEA in female mice. Methods: Ninety 8-week-old female mice were randomly allocated into three treatment groups. The first group received an intraperitoneal injection of ZEA (2.5 mg/kg) on alternate days. The second group received ZEA (2.5 mg/kg) on alternate days plus oral saffron daily (50 mg/kg). The third group was treated with a vehicle of 1% dimethyl sulfoxide (DMSO) on alternate days, as a control. Ten mice were euthanized from each group at 30, 60, and 90 days of treatment. Serum levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), estradiol ($E_2$), and progesterone (P) were assessed. The uterus and ovaries were examined for changes in size or morphology. Results: Serum levels of LH, FSH, $E_2$, and P in the female mice treated with ZEA plus saffron were significantly higher than in those treated with ZEA alone, and were not significantly different from those treated with 1% DMSO. The female mice treated with ZEA alone showed a reduction in size of the uterus and abnormal architecture of the ovaries. Conclusion: The administration of saffron to female mice resulted in a significant reduction in ZEA-induced alterations in reproductive hormone levels, the size of the uterus, and the morphology of the ovaries.

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  1. Detoxification Strategies for Zearalenone Using Microorganisms: A Review vol.7, pp.7, 2018, https://doi.org/10.3390/microorganisms7070208
  2. Effects of zearalenone on genital organ development, serum immunoglobulin, antioxidant capacity, sex hormones and liver function of prepubertal gilts vol.189, pp.None, 2018, https://doi.org/10.1016/j.toxicon.2020.11.005
  3. Scutellarin protects mouse ovarian granulosa cells from injury induced by the toxin zearalenone vol.12, pp.3, 2018, https://doi.org/10.1039/d0fo02711a
  4. Impact of Fusarium-Derived Mycoestrogens on Female Reproduction: A Systematic Review vol.13, pp.6, 2018, https://doi.org/10.3390/toxins13060373
  5. The Effects of a Saffron Extract (affron®) on Menopausal Symptoms in Women during Perimenopause: A Randomised, Double-Blind, Placebo-Controlled Study vol.27, pp.2, 2021, https://doi.org/10.6118/jmm.21002
  6. Food Mycotoxins: Dietary Interventions Implicated in the Prevention of Mycotoxicosis vol.1, pp.10, 2018, https://doi.org/10.1021/acsfoodscitech.1c00220
  7. Network pharmacology-based study on the mechanism of scutellarin against zearalenone-induced ovarian granulosa cell injury vol.227, pp.None, 2018, https://doi.org/10.1016/j.ecoenv.2021.112865