Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Detection of Cryptosporidium spp. in Diarrheic Immunocompetent Patients in Beni-Suef, Egypt: Insight into Epidemiology and Diagnosis

  • Gawad, Samah S. Abdel (Parasitology Department, Faculty of Medicine, Beni-Suef University) ;
  • Ismail, Mousa A.M. (Parasitology Department, Faculty of Medicine, Cairo University) ;
  • Imam, Naglaa F.A. (Parasitology Department, Faculty of Medicine, Cairo University) ;
  • Eassa, Ahmed H.A. (Parasitology Department, Faculty of Medicine, Cairo University) ;
  • abu-Sarea, Enas Yahia (Parasitology Department, Faculty of Medicine, Beni-Suef University)
  • Received : 2017.10.06
  • Accepted : 2018.04.17
  • Published : 2018.04.30
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Abstract

Cryptosporidium species is an important cause of gastrointestinal infections globally. This study aimed to shed light on its role in diarrheic immunocompetent patients in Beni-Suef, Egypt and to compare three diagnostic methods. Two hundred diarrheic patients, $37{\pm}16.8year$ old, were enrolled. Stool samples were examined by light microscopy, using modified Ziehl-Neelsen stain (MZN) for Cryptosporidium spp. oocysts. Coproantigens were detected by sandwich ELISA. DNA molecular diagnosis was done by nested PCR. PCR yielded the highest detection rates (21.0%), compared to ELISA (12.5%) and MZN staining method (9.5%). The higher infection rates were in 20-40 year-old group, followed by 40-60 year-old. Association between epidemiologic factors was statistically not significant; positivity and gender, clinical manifestations, residence, source or water, or contact with animals. Cryptosporidiosis is an important enteric parasitic infection in Beni-Suef and PCR remains the gold standard for diagnosis.

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