Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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A New Method of Producing a Natural Antibacterial Peptide by Encapsulated Probiotics Internalized with Inulin Nanoparticles as Prebiotics

  • Cui, Lian-Hua (Department of Animal Science, College of Agriculture Science, Yanbian University) ;
  • Yan, Chang-Guo (Department of Animal Science, College of Agriculture Science, Yanbian University) ;
  • Li, Hui-Shan (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University) ;
  • Kim, Whee-Soo (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University) ;
  • Hong, Liang (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University) ;
  • Kang, Sang-Kee (Institute of Green-Bio Science and Technology, Seoul National University) ;
  • Choi, Yun-Jaie (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University) ;
  • Cho, Chong-Su (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University)
  • Received : 2017.12.06
  • Accepted : 2018.01.19
  • Published : 2018.04.28
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Abstract

Synbiotics are a combination of probiotics and prebiotics, which lead to synergistic benefits in host welfare. Probiotics have been used as an alternative to antibiotics. Among the probiotics, Pediococcus acidilactici (PA) has shown excellent antimicrobial activity against Salmonella Gallinarum (SG) as a major poultry pathogen and has improved the production performances of animals. Inulin is widely used as a prebiotic for the improvement of animal health and growth. The main aim of this study was to investigate the antimicrobial activity of inulin nanoparticle (IN)-internalized PA encapsulated into alginate/chitosan/alginate (ACA) microcapsules (MCs) for future in vivo application. The prepared phthalyl INs (PINs) were characterized by DLS and FE-SEM. The contents of phthal groups in the PINs were estimated by $^1H-NMR$ measurement as 25.1 mol.-%. The sizes of the PINs measured by DLS were approximately 203 nm. Internalization into PA was confirmed by confocal microscopy and flow cytometry. The antimicrobial activity of PIN-internalized probiotics encapsulated into ACA MCs was measured by coculture antimicrobial assays on SG. PIN-internalized probiotics had a higher antimicrobial ability than that of ACA MCs loaded with PA/inulin or PA. Interestingly, when PINs were treated with PA and encapsulated into ACA MCs, as a natural antimicrobial peptide, pediocin was produced much more in the culture medium compared with other groups with inulin-loaded ACA MCs and PA encapsulated into ACA MCs.

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References

  1. Allen HK, Levine UY, Looft T, Bandrick M, Casey TA. 2013. Treatment, promotion, commotion: antibiotic alternatives in food-producing animals. Trends Microbiol. 21: 114-119. https://doi.org/10.1016/j.tim.2012.11.001
  2. Stanton TB. 2013. A call for antibiotic alternatives research. Trends Microbiol. 21: 111-113. https://doi.org/10.1016/j.tim.2012.11.002
  3. Jiang T, Li HS, Han GG, Singh B, Kang SK, Bok JD, et al. 2017. Oral delivery of probiotics in poultry using pH-sensitive tablets. J. Microbiol. Biotechnol. 27: 739-746. https://doi.org/10.4014/jmb.1606.06071
  4. Lee SH, Lillehoj HS, Dalloul RA, Park DW, Hong YH, Lin JJ. 2007. Influence of Pediococcus-based probiotic on coccidiosis in broiler chickens. Poult. Sci. 86: 63-66. https://doi.org/10.1093/ps/86.1.63
  5. Jin LZ, Ho YW, Abdullah N, Jalaludin S. 2000. Digestive and bacterial enzyme activities in broilers fed diets supplemented with Lactobacillus cultures. Poult. Sci. 79: 886-891. https://doi.org/10.1093/ps/79.6.886
  6. Cunningham-Rundles S, Ahrne S, Bengmark S, Johann-Liang R, Marshall F, Metakis L, et al. 2000. Probiotics and immune response. Am. J. Gastroenterol. 95: S22-S25. https://doi.org/10.1016/S0002-9270(99)00813-8
  7. Gibson GR, Roberfroid MB. 1995. Dietary modulation of the human colonic microbiota - introducing the concept of prebiotics. J. Nutr. 125: 1401-1412.
  8. Gourbeyre P, Denery S, Bodinier M. 2011. Probiotics, prebiotics, and synbiotics: impact on the gut immune system and allergic reactions. J. Leukoc. Biol. 89: 685-695. https://doi.org/10.1189/jlb.1109753
  9. Farthing MJG. 2004. Microbial-gut interactions in health and disease - preface. Best Pract. Res. Clin. Gastroenterol 18: 231.
  10. Schwiertz A. 2016. Microbiota of the human body implications in health and disease. Preface. Adv. Exp. Med. Biol. 902: V.
  11. Scott KP, Martin JC, Duncan SH, Flint HJ. 2014. Prebiotic stimulation of human colonic butyrate-producing bacteria and bifidobacteria, in vitro. FEMS Microbiol. Ecol. 87: 30-40. https://doi.org/10.1111/1574-6941.12186
  12. Petros RA, DeSimone JM. 2010. Strategies in the design of nanoparticles for therapeutic applications. Nat. Rev. Drug Discov. 9: 615-627. https://doi.org/10.1038/nrd2591
  13. Elsabahy M, Wooley KL. 2012. Design of polymeric nanoparticles for biomedical delivery applications. Chem. Soc. Rev. 41: 2545-2561. https://doi.org/10.1039/c2cs15327k
  14. Bajracharya P, Islam MA, Jiang T, Kang SK, Choi YJ, Cho CS. 2012. Effect of microencapsulation of Lactobacillus salivarus 29 into alginate/chitosan/alginate microcapsules on viability and cytokine induction. J. Microencapsul. 29: 429-436. https://doi.org/10.3109/02652048.2012.655332
  15. Islam MA, Yun CH, Choi YJ, Cho CS. 2010. Microencapsulation of live probiotic bacteria. J. Microbiol. Biotechnol. 20: 1367-1377. https://doi.org/10.4014/jmb.1003.03020
  16. Islam MA, Bajracharya P, Kang SK, Yun CH, Kim EM, Jeong HJ, et al. 2011. Mucoadhesive alginate/poly (L-lysine)/thiolated alginate microcapsules for oral delivery of Lactobacillus salivarius 29. J. Nanosci. Nanotechnol. 11: 7091-7095. https://doi.org/10.1166/jnn.2011.4858
  17. Kumar S, Kesharwani SS, Kuppast B, Rajput M, Bakkari MA, Tummala H. 2016. Discovery of inulin acetate as a novel immune-active polymer and vaccine adjuvant: synthesis, material characterization, and biological evaluation as a Toll-like receptor-4 agonist. J. Mater. Chem. B 4: 7950-7960.
  18. Jiang T, Singh B, Maharjan S, Li HS, Kang SK, Bok JD, et al. 2014. Oral delivery of probiotic expressing M cell homing peptide conjugated BmpB vaccine encapsulated into alginate/chitosan/alginate microcapsules. Eur. J. Pharm. Biopharm. 88: 768-777. https://doi.org/10.1016/j.ejpb.2014.07.003
  19. Jiang T, Kim YK, Singh B, Kang SK, Choi YJ, Cho CS. 2013. Effect of microencapsulation of Lactobacillus plantarum 25 into alginate/chitosan/alginate microcapsules on viability and cytokine induction. J. Nanosci. Nanotechnol. 13: 5291-5295. https://doi.org/10.1166/jnn.2013.7042
  20. Li XY, Chen XG, Cha DS, Park HJ, Liu CS. 2009. Microencapsulation of a probiotic bacteria with alginate-gelatin and its properties. J. Microencapsul. 26: 315-324. https://doi.org/10.1080/02652040802328685
  21. Ditu LM, Chifiriuc MC, Bezirtzoglou E, Voltsi C, Bleotu C, Pelinescu D, et al. 2011. Modulation of virulence and antibiotic susceptibility of enteropathogenic Escherichia coli strains by Enterococcus faecium probiotic strain culture fractions. Anaerobe 17: 448-451. https://doi.org/10.1016/j.anaerobe.2011.05.019
  22. Altuntas EG, Ayhan K, Peker S, Ayhan B, Demiralp DO. 2014. Purification and mass spectrometry based characterization of a pediocin produced by Pediococcus acidilactici 13. Mol. Biol. Rep. 41: 6879-6885. https://doi.org/10.1007/s11033-014-3573-z
  23. Chiba Y, Shida K, Nagata S, Wada M, Bian L, Wang CX, et al. 2010. Well-controlled proinflammatory cytokine responses of Peyer's patch cells to probiotic Lactobacillus casei. Immunology 130: 352-362. https://doi.org/10.1111/j.1365-2567.2009.03204.x
  24. Kim WS, Lee JY, Singh B, Maharjan S, Hong L, Lee SM, et al. 2017. A new way of producing pediocin in Pediococcus acidilactici through intracellular stimulation by internalized inulin nanoparticles. Scientific Reports In press
  25. Sanyasi S, Majhi RK, Kumar S, Mishra M, Ghosh A, Suar M, et al. 2016. Polysaccharide-capped silver Nanoparticles inhibit biofilm formation and eliminate multi-drug-resistant bacteria by disrupting bacterial cytoskeleton with reduced cytotoxicity towards mammalian cells. Sci. Rep. 6: 24929. https://doi.org/10.1038/srep24929
  26. Hamilton-Miller JM. 2004. Probiotics and prebiotics in the elderly. Postgrad. Med. J. 80: 447-451. https://doi.org/10.1136/pgmj.2003.015339
  27. Hanson RW, Ballard FJ. 1967. The relative significance of acetate and glucose as precursors for lipid synthesis in liver and adipose tissue from ruminants. Biochem. J. 105: 529-536. https://doi.org/10.1042/bj1050529
  28. Zambell KL, Fitch MD, Fleming SE. 2003. Acetate and butyrate are the major substrates for de novo lipogenesis in rat colonic epithelial cells. J. Nutr. 133: 3509-3515. https://doi.org/10.1093/jn/133.11.3509
  29. Fukuda S, Toh H, Hase K, Oshima K, Nakanishi Y, Yoshimura K, et al. 2011. Bifidobacteria can protect from enteropathogenic infection through production of acetate. Nature 469: 543-547. https://doi.org/10.1038/nature09646
  30. Foegeding PM, Thomas AB, Pilkington DH, Klaenhammer TR. 1992. Enhanced control of Listeria monocytogenes by in situ-produced pediocin during dry fermented sausage production. Appl. Environ. Microbiol. 58: 884-890.
  31. Nielsen JW, Dickson JS, Crouse JD. 1990. Use of a bacteriocin produced by Pediococcus acidilactici to inhibit Listeria monocytogenes associated with fresh meat. Appl. Environ. Microbiol. 56: 2142-2145.
  32. Pucci MJ, Vedamuthu ER, Kunka BS, Vandenbergh PA. 1988. Inhibition of Listeria monocytogenes by using bacteriocin Pa-1 produced by Pediococcus acidilactici Pac 1.0. Appl. Environ. Microbiol. 54: 2349-2353.

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