Journal of Naturopathy (한국자연치유학회지)

Koean Soceity for Naturopathy (한국자연치유학회)
권호 표지

Antioxidant and Anti-aging Activities of Ethanol Extracts from Defatted Perilla frutescens

들깨박 추출물의 항산화 및 항노화 효과

  • Kang, Hyun (Department of Medical Laboratory Science, College of Health Science, Dankook University) ;
  • Lee, Sung-Gyu (Department of Medical Laboratory Science, College of Health Science, Dankook University) ;
  • Song, Gap-Jung (NPRL Inc.) ;
  • Jung, Mon-Seok (Medwell Inc.)
  • 강현 (단국대학교 보건과학대학 임상병리학과) ;
  • 이성규 (단국대학교 보건과학대학 임상병리학과) ;
  • 송갑정 (주식회사 엔피알엘) ;
  • 정문석 (메드웰)
  • Received : 2018.09.18
  • Accepted : 2018.09.21
  • Published : 2018.09.30
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Abstract

Purpose: This study was conducted to investigate the antioxidative, collagenase and elastase activities of defatted perilla ethanol extracts. In addition, the possibility of its use as a functional cosmetic material has been studied. Methods: In order to investigate the antioxidant function of the perilla extract, electron spin resonance (ESR) was measured with a spectrometer by analyzing the scavenging ability of diphenyl-β-picrylhydrazyl (DPPH) free radicals. Results: The antioxidant activity of DPPH free radical scavenging activity of the perilla extract was about 68% at 85 ㎍/ml, 85% at 20 ㎍/ml, 90% at 40-100 ㎍/ml, and showed antioxidant ability. The inhibitory activity of collagenase was increased to 4.1% at 20 mg/ml, 12% at 40 mg/ml and 26.7% at 100 mg/ml. The inhibitory activity increased in proportion to the concentration. The inhibitory activity of elastase was increased to 3.8% at 20 mg/ml, 15% at 40 mg/ml, 28% at 80 mg/ml and 35.7% at 100 mg/ml. Conclusions: These results suggest that the ethanol extract of perilla may inhibit the antioxidant activity and the activity of collagenase and elastase to improve the skin aging and wrinkles.

목적: 본 연구는 들깨 부산물의 이용가치를 높이는 방법으로 들깨박 에탄올 추출물의 항산화 작용, collagenase와 elastase 활성의 저해작용을 통하여 항노화 활성이 있는지를 연구하였다. 그 외에 기능성 화장품 소재로서의 이용가능성을 연구도하였다. 방법: 들깨박 추출물의 항산화 기능을 연구하기 위하여 DPPH 자유기의 소거능력을 분석하여 electron spin resonance (ESR)를 광도계로 측정하였다. 결과: 들깨박의 에탄올 추출물의 diphenyl-β-picrylhydrazyl (DPPH) 자유기 소거의 항산화 기능은 추출물 농도 10 ㎍/ml에서는 약 68%, 20 ㎍/ml에서는 85%, 40-100 ㎍/ml에서는 90% 이상의 자유기의 소거 활성을 보여서 항산화능이 있음을 나타냈다. collagenase의 저해활성은 에탄올 추출물 농도 20 mg/ml에서는 4.1%, 40 mg/ml에서는 12%, 100 mg/ml에서는 26.7%로 증가하였다. 저해활성이 농도에 비례하여 증가하였다. elastase의 저해활성은 에탄올 추출물 농도 20 mg/ml에서는 3.8%, 40 mg/ml에서는 15%, 80 mg/ml에서는 28%, 100 mg/ml에서는 35.7%로 증가하였다. 결론: 이상의 연구결과에서 들깨박의 에탄올 추출물이 항산화효과, collagenase와 elastase의 활성을 저해하여 피부의 노화와 주름의 개선효과를 나타낼 수 있다고 본다.

Keywords

Acknowledgement

본 연구는 중소벤처기업부의 창업성장기술개발사업의 일환으로 수행하였음(S2539864, 국내 다유들깨 부산물로부터 광노화 소재 발굴 및 스킨디펜스 화장품개발).

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