생명과학회지 (Journal of Life Science)

  • 제28권4호
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  • Pages.454-464
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  • 2018
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

HaCaT 인간 피부 각질세포에서 과산화수소 유도 산화 손상에 대한 소청자 및 소총2호의 항산화 및 세포보호 효능

Antioxidant and Cytoprotective Effects of Socheongja and Socheong 2, Korean Black Seed Coat Soybean Varieties, against Hydrogen Peroxide-induced Oxidative Damage in HaCaT Human Skin Keratinocytes

  • 최은옥 (동의대학교 한의과대학 생화학교실 및 근.골격계 질환제어 융합연구실) ;
  • 권다혜 (동의대학교 항노화연구소) ;
  • 황혜진 (동의대학교 의료.보건.생활대학 식품영양학과) ;
  • 김국진 (제노마인(주) 첨단생명공학연구소) ;
  • 이동희 (제노마인(주) 첨단생명공학연구소) ;
  • 최영현 (동의대학교 한의과대학 생화학교실 및 근.골격계 질환제어 융합연구실)
  • Choi, Eun Ok (Open Laboratory for Muscular & Skeletal Disease Control and Department of Biochemistry, Dongeui University College of Korean Medicine) ;
  • Kwon, Da Hye (Anti-Aging Research Center, Dongeui University) ;
  • Hwang, Hye-Jin (Department of Food and Nutrition, College of Natural Sciences and Human Ecology, Dongeui University) ;
  • Kim, Kook Jin (Genomine Advanced Biotechnology Research Institute, Genomine Inc.) ;
  • Lee, Dong Hee (Genomine Advanced Biotechnology Research Institute, Genomine Inc.) ;
  • Choi, Yung Hyun (Open Laboratory for Muscular & Skeletal Disease Control and Department of Biochemistry, Dongeui University College of Korean Medicine)
  • 투고 : 2017.10.31
  • 심사 : 2017.11.18
  • 발행 : 2018.04.30
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초록

식품 원료로도 널리 애용되는 검은콩은 풍부한 천연 페놀 화합물을 함유하고 있기 때문에 기능성 소재로서의 개발에도 매우 유용한 자원이다. 본 연구에서는 3가지 검은콩 품종[소청자(SCJ), 소청2호(SC2) 및 청자2호(CJ2)]을 대상으로 TPCs과 항산화 능을 조사하였다. 그 중에서도 TPCs는 CJ2 $H_2O_2$ 처리에 의한 HaCaT 세포의 생존력 감소를 현저히 억제하여 산화적 스트레스에 대한 보호 효과가 있음을 알 수 있었다. SCJ와 SC2 전처리는 또한 HaCaT 세포에서 mitochondrial dysfunction의 차단과 pro-apoptotic Bax의 발현 변화의 정상화를 통해 $H_2O_2$에 의하여 유도된 apoptosis를 효과적으로 억제하였으며, DNA 손상에 대한 보호 효과와 연관성이 있었다. 또한 SCJ와 SC2는 Nrf2와 연관된 TrxR1의 발현을 효과적으로 유도하였으나, 산화적 스트레스에 대한 SCJ와 SC2의 보호 효과는 TrxR 억제제에 의하여 상쇄되었다. 이러한 결과는 SCJ와 SC2가 Nrf2 신호전달 경로 활성을 통하여 산화적 스트레스와 관련된 세포 손상을 차단함으로써 세포 보호 활성을 갖는다는 것을 의미한다. 결론적으로, SCJ와 SC2는 산화스트레스로 인한 피부 질환의 치료와 예방을 위한 응용 가능성이 높음을 보여주었다.

Black soybeans are used as food sources as well as for traditional medicines because they contain an abundance of natural phenolic compounds. In this study, total phenolic contents (TPCs) of Korean black seed coat soybean varieties Socheongja (SCJ), Socheong 2 (SC2) and Cheongja 2 (CJ2) as well as their antioxidant capacities were investigated. Among them, TPCs were abundantly present in the order of CJ2$H_2O_2$-stimulated HaCaT human keratinocytes. Our results revealed that treatment with SCJ and SC2 prior to $H_2O_2$ exposure significantly increases the viability of HaCaT cells, indicating that the exposure of HaCaT cells to SCJ and SC2 conferred a protective effect against oxidative stress. SCJ and SC2 also effectively inhibited $H_2O_2$-induced apoptotic cell death through the blocking of mitochondrial dysfunction. SCJ and SC2 also attenuated the phosphorylation of Histone H2AX. Furthermore, they effectively induced the levels of thioredoxin reductase (TrxR) 1, a potent antioxidant enzyme, which is associated with the induction of nuclear transcription factor erythroid-2-like factor 2 (Nrf2); however, the protective effects of SCJ and SC2 were significantly reversed by Auranofin, a TrxR inhibitor. These results indicate that they have protective activity through the blocking of cellular damage related to oxidative stress via the Nrf2 signaling pathway. In conclusion, our study indicated that SCJ and SC2 might potentially serve as novel agents for the treatment and prevention of skin disorders caused by oxidative stress.

키워드

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