한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제26권2호
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  • Pages.127-133
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  • 2018
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
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감마선 조사 처리에 의한 결명자 줄기의 전처리와 효소가수분해 효과

Effect of Gamma Ray Irradiation on the Pretreatment and Enzymatic Hydrolysis of Senna tora Stalk

  • 김조은 (전남대학교 산림자원학부) ;
  • 공성호 (전남대학교 산림자원학부) ;
  • 정진태 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이옥란 (전남대학교 식물생명공학부) ;
  • 이재원 (전남대학교 산림자원학부)
  • Kim, Jo Eun (Department of Forest Resources, Chonnam National University) ;
  • Gong, Sung Ho (Department of Forest Resources, Chonnam National University) ;
  • Jung, Jin Tae (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Ok Ran (Department of Plant Biotechnology, Chonnam National University) ;
  • Lee, Jae Won (Department of Forest Resources, Chonnam National University)
  • 투고 : 2017.08.28
  • 심사 : 2017.09.18
  • 발행 : 2018.04.30
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초록

Background: The demand of recycling renewable agricultural by-products is increasing. Radiation breeding is a method used to improve saccharification efficiency. Thus, we investigated the effect of gamma ray irradiation on the pretreatment and enzymatic hydrolysis of the stalks of Senna tora, an important medicinal plants. Methods and Results: S. tora seeds were irradiated with gamma ray at doses of 100, 200, 300, and 400 Gy. In the pretreated biomass, glucan and lignin content were higher in the M1 ($1^{st}$ generations of irradiation) S. tora stalks than in the M2 ($2^{nd}$ generations of irradiation) stalks, this can be explained by the higher degradation rate in M1. After oxalic acid pretreatment, the concentration of total phenolic compounds (TPCs) in the hydrolysate increased in the gamma ray treated seeds. The highest relative increase rate in crystallinity in the pretreated biomass was observed in M1-400 Gy and M2-100 Gy. The cellulose conversion rate was higher in M1 than in M2, except for 200 Gy. Conclusions: Gamma ray irradiation at an appropriate dose can be used to improve the efficiency of pretreatment and enzymatic hydrolysis, thereby increasing biomass availability.

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