Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Collagen-Induced Arthritis Analysis in Rhbdf2 Knockout Mouse

  • Lee, Min-Young (Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kang, Ju-Seong (Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Go, Ryeo-Eun (Laboratory of Biochemistry and Immunology, College of Veterinary Medicine, Chungbuk National University) ;
  • Byun, Yong-Sub (Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Wi, Young Jin (Department of Life Science, College of Natureal Sciences, Research Institute of Natural Sciences, Hanyang University) ;
  • Hwang, Kyung-A (Laboratory of Biochemistry and Immunology, College of Veterinary Medicine, Chungbuk National University) ;
  • Choi, Jae-Hoon (Department of Life Science, College of Natureal Sciences, Research Institute of Natural Sciences, Hanyang University) ;
  • Kim, Hyoung-Chin (Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Choi, Kyung-Chul (Laboratory of Biochemistry and Immunology, College of Veterinary Medicine, Chungbuk National University) ;
  • Nam, Ki-Hoan (Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology)
  • Received : 2017.05.07
  • Accepted : 2017.07.24
  • Published : 2018.05.01
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Abstract

Rhomboid family member 2 gene (Rhbdf2) is an inactive homologue lacking essential catalytic residues of rhomboid intramembrane serine proteases. The protein is necessary for maturation of tumor necrosis factor-alpha ($TNF-{\alpha}$) converting enzyme, which is the molecule responsible for the release of $TNF-{\alpha}$. In this study, Rhbdf2 knockout (KO) mice were produced by CRISPR/CAS9. To see the effects of the failure of $TNF-{\alpha}$ release induced by Rhbdf2 gene KO, collagen-induced arthritis (CIA), which is the representative $TNF-{\alpha}$ related disease, was induced in the Rhbdf2 mutant mouse using chicken collagen type II. The severity of the CIA was measured by traditional clinical scores and histopathological analysis of hind limb joints. A rota-rod test and grip strength test were employed to evaluate the severity of CIA based on losses of physical functions. The results indicated that Rhbdf2 mutant mice showed clear alleviation of the clinical severity of CIA as demonstrated by the significantly lower severity indexes. Moreover, a grip strength test was shown to be useful for the evaluation of physical functional losses by CIA. Overall, the results showed that the Rhbdf2 gene has a significant effect on the induction of CIA, which is related to $TNF-{\alpha}$.

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References

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