한국균학회지 (The Korean Journal of Mycology)

  • 제46권4호
  • /
  • Pages.405-414
  • /
  • 2018
  • /
  • 0253-651X(pISSN)
  • /
  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
권호 표지
DOI QR코드

DOI QR Code

제주도에 서식하는 식물 잎에서 분리된 6종의 국내 미기록 내생균 보고

Characterization of Six Novel Endophytic Fungi Isolated from Leaves of Plants Inhabiting Jeju Island

  • 박혁 (한국교원대학교 생물교육과) ;
  • 최영준 (군산대학교 생물학과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Park, Hyeok (Department of Biology Education, Korea National University of Education) ;
  • Choi, Young-Joon (Department of Biology, Kunsan National University) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 투고 : 2018.11.15
  • 심사 : 2018.11.19
  • 발행 : 2018.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

제주도에 서식하는 다양한 식물의 잎에서 내생균을 분리하였다. 분리된 균주들은 형태적 특성 및 internal transcribed spacer, large subunit rDNA 영역 및 beta-tubulin rDNA 유전자의 염기서열의 계통 분석을 통해 종을 동정하였다. 그 결과 6종의 국내 미기록 내생균을 확인하였고, 확인된 종은 각각 Diaporthe goulteri, Diaporthe vaccini, Rhizosphaera pini, Valsa friesii, Xylaria primorskensis, Zalerion arboricola 이다. 확인된 6종의 미기록 내생균 균주의 형태적 특성 및 염기서열 계통분석의 결과에 대해 기술하였다.

Endophytic fungi were isolated from the leaves of diverse plants inhabiting Jeju Island, Korea. The fungal isolates were identified through phylogenetic analyses incorporating nucleotide sequences derived from the internal transcribed spacer region, large subunit region of ribosomal DNA, and beta-tubulin gene. Our results identified six endophytic fungi previously unknown in Korea namely, Diaporthe goulteri, Diaporthe vaccini, Rhizosphaera pini, Valsa friesii, Xylaria primorskensis, and Zalerion arboricola were identified. Here, we present their cultural and morphological characteristics and phylogenetic relationship.

키워드

GNHHDL_2018_v46n4_405_f0001.png 이미지

Fig. 1. Colonies of strain P8 (Diaporthe goulteri) grown for 7 days on PDA (A) and MEA (G), conidia (M). Colonies of strain 17E026 (Diaporthe vaccinii) grown for 7 days on PDA (B) and MEA (H). Colonies of strain 16H073 (Rhizosphaera pini) grown for 7 days on PDA (C) and MEA (I), conidiophore and conidium (N, O). Colonies of strain 16H342 (Valsa friesii) grown for 7 days on PDA (D) and MEA (J). Colonies of strain 17E107 (Xylaria primorskensis) grown for 7 days on PDA (E) and MEA (K), conidia (P). Colonies of strain 16H107 (Zalerion arboricola) grown for 7 days on PDA (F) and MEA (L), conidiophore and conidia (Q, R). PDA, potato dextrose agar; MEA, malt extract agar (scale bar = 10 μm).

GNHHDL_2018_v46n4_405_f0002.png 이미지

Fig. 2. Neighbor-joining phylogenetic tree based on a combined alignment of internal transcribed spacer, large subunit rDNA, and beta-tubulin rDNA sequences. Nectria dacryocarpa was used as an outgroup. Numbers on branches indicate bootstrap values (1,000 replicates). Bold letters indicates fungal strains isolated in this study.

GNHHDL_2018_v46n4_405_f0003.png 이미지

Fig. 3. Neighbor-joining phylogenetic tree based on a combined alignment of internal transcribed spacer and large subunit rDNA sequences. Phomopsis tuberivora was used as an outgroup. Numbers on branches indicate bootstrap values (1,000 replicates). Bold letters indicates fungal strains isolated in this study.

GNHHDL_2018_v46n4_405_f0004.png 이미지

Fig. 4. Neighbor-joining phylogenetic tree based on a combined alignment of internal transcribed spacer and large subunit rDNA sequences. Diaporthe acaciarum was used as an outgroup. Numbers on branches indicate bootstrap values (1,000 replicates. Bold letters indicates fungal strains isolated in this study.

GNHHDL_2018_v46n4_405_f0005.png 이미지

Fig. 5. Neighbor-joining phylogenetic tree based on a combined alignment of internal transcribed spacer and large subunit rDNA sequences. Botryosphaeria dothidea was used as an outgroup. Numbers on branches indicate bootstrap values (1,000 replicates). Bold letters indicates fungal strains isolated in this study.

GNHHDL_2018_v46n4_405_f0006.png 이미지

Fig. 6. Neighbor-joining phylogenetic tree based on a combined alignment of internal transcribed spacer and large subunit rDNA sequences. Glonium stellatum was used as an outgroup. Numbers on branches indicate bootstrap values (1,000 replicates). Bold letters indicates fungal strains isolated in this study.

참고문헌

  1. Carroll G. Fungal endophytes in stems and leaves: from latent pathogen to mutualistic symbiont. Ecology 1988;69:2-9. https://doi.org/10.2307/1943154
  2. Saikkonen K, Faeth SH, Helander M, Sullivan TJ. Fungal endophytes: a continuum of interactions with host plants. Annu Rev Ecol Syst 1998;29:319-43. https://doi.org/10.1146/annurev.ecolsys.29.1.319
  3. Strobel GA, Hess WM, Ford E, Sidhu RS, Yang X. Taxol from fungal endophytes and the issue of biodiversity. J Ind Microbiol 1996;17:417-23.
  4. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. San Diego: Academic Press; 1990. p. 315-22.
  5. Moncalvo JM, Lutzoni FM, Rehner SA, Johnson J, Vilgalys R. Phylogenetic relationships of agaric fungi based on nuclear large subunit ribosomal DNA sequences. Syst Biol 2000;49:278-305. https://doi.org/10.1093/sysbio/49.2.278
  6. Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 1995;61:1323-30.
  7. Thompson SM, Tan YP, Shivas RG, Neate SM, Morin L, Bissett A, Aitken EA. Green and brown bridges between weeds and crops reveal novel Diaporthe species in Australia. Persoonia 2015;35:39-49. https://doi.org/10.3767/003158515X687506
  8. Kanematsu S, Kobayashi T, Kudo A, Ohtsu Y. Conidial morphology, pathogenicity and culture characteristics of Phomopsis isolates from peach, Japanese pear and apple in Japan. Annu Phytopathol Soc Jpn 1999;65:264-73. https://doi.org/10.3186/jjphytopath.65.264
  9. Shear CL, Stevens NE, Bain HF. Fungous diseases of the cultivated cranberry. Washington, DC: US Department of Agriculture; 1931.
  10. Tanney JB, McMullin DR, Green BD, Miller JD, Seifert KA. Production of antifungal and antiinsectan metabolites by the Picea endophyte Diaporthe maritima sp. nov. Fungal Biol 2016;120:1448-57. https://doi.org/10.1016/j.funbio.2016.05.007
  11. Dos Santos TT, de Souza Leite T, de Queiroz CB, de Araujo EF, Pereira OL, de Queiroz MV. High genetic variability in endophytic fungi from the genus Diaporthe isolated from common bean (Phaseolus vulgaris L.) in Brazil. J Appl Microbiol 2016;120:388-401. https://doi.org/10.1111/jam.12985
  12. Su H, Kang JC, Cao JJ, Mo L, Hyde KD. Medicinal plant endophytes produce analogous bioactive compounds. Chiang Mai J Sci 2014;41:1-13.
  13. Maublanc MA. Sur la maladie des sapins produite par le Fusicoccum abietinum. Bull Soc Myc France 1907;23:160-73.
  14. Fuckel L. Symbolae mycologicae: Beitrage zur Kenntnis der rheinischen Pilze. Lehre: J. Cramer; 1966.
  15. Fries E. Summa vegetabilium SCANDINAVIAE II. Upsaliae. Uppsala: Typographia Academica; 1849.
  16. Child JJ, Nesbitt LR, Haskins RH. Production of m-Cresol by Valsa friesii. Appl Microbiol 1969;18:515-6.
  17. Ju YM, Hsieh HM, Vasilyeva L, Akulov A. Three new Xylaria species from Russian Far East. Mycologia 2009;101:548-53. https://doi.org/10.3852/08-188
  18. Adnan M, Patel M, Reddy MN, Alshammari E. Formulation, evaluation and bioactive potential of Xylaria primorskensis terpenoid nanoparticles from its major compound xylaranic acid. Sci Rep 2018;8:1740. https://doi.org/10.1038/s41598-018-20237-z
  19. Bugzacki ST. Zalerion arboricola, a new helicosporous hyphomycete from conifer stems. Trans Br Mycol Soc 1972;59:159-61, IN22. https://doi.org/10.1016/S0007-1536(72)80056-1