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배검은별무늬병균과 배붉은별무늬병균에 대한 유기농자재들의 항균활성

Antifungal Activity of Agro-Materials against Pear Scab (Venturia nashicola) and Pear Rust (Gymnosporangium asiaticum) Fungi

  • 송장훈 (농촌진흥청 국립원예특작과학원 배연구소) ;
  • 서호진 (농촌진흥청 국립원예특작과학원 배연구소)
  • Song, Janghoon (Pear Research Institute, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Seo, Ho-Jin (Pear Research Institute, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 투고 : 2017.12.20
  • 심사 : 2018.02.08
  • 발행 : 2018.03.31

초록

본 연구는 무기살균제, 식물추출물, 미생물 등 우리나라에 등록된 병해관리용 유기농자재 중 19종에 대해 배검은별무늬병균과 배붉은별무늬병균을 접종하고 배 잎 조직에서의 항균활성을 확인하고자 수행하였다. 배검은별무늬병균에 대해 황, 구리 등이 함유된 유기농자재 9종 중에서 대부분은 포자발아를 완전히 억제하였고, 일부 발아한 포자의 경우에도 부착기가 전혀 형성되지 않았으나 배붉은별무늬병균에 대해서는 석회황합제, 네오보르도, 흰가리스 등에 한하여 항균활성을 보였다. 식물추출물을 함유하는 유기농자재 중 흰가루자바는 배 검은 별무늬병균의 분생포자 발아를 완전하게 억제하였으며 배 붉은별무늬병균에 대해서도 71.6% 수준으로 비교적 높은 포자발아 억제효과를 보였다. 미생물 함유 제제로써 청고탄(Streptomyces griseus)은 배검은별무늬병균에 대해 88.8%의 포자발아억제율을 보이고 부착기도 형성하지 않으며 세포간에 자재집적이 확인되지 않았다. 탑시드(Paenibacillus polymyxa)는 배검은별무늬병균과 배 붉은별무늬병균에 대해 포자발아억제율이 각각 71.0%와 90.6% 수준이며 부착기형성도 이뤄지지 않았다. 향후 직접적인 접촉으로 인하여 발생되는 보호효과뿐만 아니라 미생물 대사산물과 천연화합물이 지니고 있는 유도저항성 효과도 고려하여 포장조건에서 누적적으로 살포하면서 종합적인 항균활성을 검토할 필요가 있다.

This study was conducted to evaluate the antifungal activity of 19 agro-materials that have been registered for organic cultivation in Korea, after inoculation of pear leaves with Venturia nashicola and Gymnosporangium asiaticum. In V. nashicola, most of the nine agro- materials containing sulfur and copper completely inhibited spore germination, and some of the spores that germinated did not form appressoria. However, in only lime sulfur, Neobordeaux (cupric sulfate), and Wheengaris (sulfur)showed antifungal activity against G. asiaticum. Among the agro-materials containing plant extracts, Wheengarujaba (wood vinegar+spirits+rhubarb) inhibited conidial germination in V. nashicola and G. asiaticum by 100% and 71.6%, respectively. Among the agro-materials containing antifungal microorganisms, Cheongotan (Streptomyces griseus) reduced spore germination rate of V. nashicola to 88.8%; moreover, formation of appressoria or intracellular accumulation was not observed. Application of Topsid (Paenibacillus polymyxa) reduced spore germination rates in V. nashicola and G. asiaticum to 71.0% and 90.6%, respectively, and the formation of appressoria was not observed. Studying the antifungal activity of agro-materials because of cumulative applications under the field conditions is necessary, owing to their contact fungicidal effect and the induced-resistance by microbial metabolites and natural compounds.

키워드

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