생명과학회지 (Journal of Life Science)

  • 제28권3호
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  • Pages.339-344
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  • 2018
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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오미자 박, schizandrin 및 gomisin A에 의한 RAW264.7 세포주에서 lipopolysaccharide로 유도된 염증 반응의 억제

Effects of Pomace of Schizandra chinensis, Schizandrin, and Gomisin A on LPS-induced Inflammatory Responses in RAW264.7 Cells

  • 서유미 (국립안동대학교 생명과학과) ;
  • 김현지 (국립안동대학교 생명과학과) ;
  • 이은주 (국립안동대학교 생명과학과) ;
  • 정정욱 (국립안동대학교 생명과학과) ;
  • 성화정 (국립안동대학교 식품영양학과) ;
  • 손호용 (국립안동대학교 식품영양학과) ;
  • 박종이 (경북바이오산업 연구원) ;
  • 김종식 (국립안동대학교 생명과학과)
  • Seo, Yu-Mi (Department of Biological Sciences, Andong National University) ;
  • Kim, Hyun-Ji (Department of Biological Sciences, Andong National University) ;
  • Lee, Eun-Joo (Department of Biological Sciences, Andong National University) ;
  • Chung, Chungwook (Department of Biological Sciences, Andong National University) ;
  • Sung, Hwa-Jung (Department of Food and Nutrition, Andong National University) ;
  • Sohn, Ho-Yong (Department of Food and Nutrition, Andong National University) ;
  • Park, Jong-Yi (Gyeongbuk Institute for Bio Industry) ;
  • Kim, Jong-Sik (Department of Biological Sciences, Andong National University)
  • 투고 : 2017.10.16
  • 심사 : 2017.12.04
  • 발행 : 2018.03.30
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초록

오미자는 전통적인 한약재로서 schizandrin과 gomisin A와 같은 다양한 생리활성물질을 함유하고 있는 것으로 알려져 있다. 본 연구에서는 오미자 박의 에탄올 추출물(PSC)과 schizandrin (SZ) 및 gomisin A (GA)에 의한 항염증 활성 및 그들의 작용기전 연구를 수행하였다. 먼저, PSC는 LPS에 의해 염증이 유도된 RAW264.7 세포에서 세포생존율에는 영향을 미치지 않고 농도의존적으로 nitric oxide (NO) 생성을 감소시켰다. PSC는 염증유발유전자인 iNOS와 COX-2의 발현을 감소시켰으나, $TNF-{\alpha}$의 발현에는 영향을 주지 않았다. 또한 오미자 박의 에탄올 추출물은 p38, ERK1/2 및 JNK의 총 단백질의 발현에는 영향을 주지 않으면서, 그들의 인산화를 감소시켰다. 이러한 결과는 PSC가 MAPK 신호를 저해함으로써 염증 반응을 조절할 수 있음을 시사한다. 또한 SZ와 GA도 LPS에 의해 염증이 유도된 RAW264.7 세포에서 세포 생존율에 영향을 미치지 않으면서 NO 생성을 감소시켰다. SZ은 iNOS 유전자의 발현만을 감소시킨 반면, GA는 iNOS와 COX-2 두 유전자의 발현을 모두 감소시켰다. 종합적으로 이러한 연구결과는 오미자 박 추출물, schizandrin 및 gomisin A에 의해 중재되는 항염증 활성 및 작용기전을 이해하는데 도움을 줄 것이다

Schizandra chinensis has been used as a traditional Chinese medicine and is known to have various bioactive components, including schizandrin and gomisin A. In the current study, we investigated the anti-inflammatory activities and their working mechanisms of ethanol extracts of pomace of Schizandra chinensis (PSC), schizandrin (SZ), and gomisin A (GA). First, we analyzed the effects of PSC on nitric oxide (NO) production and cell viabilities in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The results indicated that PSC dramatically reduced NO production in LPS-activated RAW264.7 cells in a dose-dependent manner without affecting cell viabilities. PSC also decreased the expression of pro-inflammatory genes iNOS and COX-2, whereas the expression of TNF-${\alpha}$ was not affected by PSC. In addition, PSC inhibited phosphorylation of p38, ERK1/2, and JNK but did not change the expression of their total protein. The results indicate that PSC can regulate LPS-induced inflammatory responses by suppressing MAPK (mitogen-activated protein kinase) signaling. We also analyzed the effects of SZ and GA on NO production and cell viabilities in RAW264.7 cells. The results showed that SZ and GA also decreased NO production in a dose-dependent manner in LPS-activated RAW 264.7 cells without affecting cell viabilities. SZ reduced the expression of iNOS, whereas GA downregulated iNOS and COX-2. Overall, these findings clarify the molecular mechanisms of the anti-inflammatory effects mediated by PSC, SZ, and GA.

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