생명과학회지 (Journal of Life Science)

  • 제28권3호
  • /
  • Pages.320-330
  • /
  • 2018
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

약용식물 5종의 용매별 추출물 및 조성물의 생리활성

Physiological Activity of Five Kinds of Medicinal Plant Extracts with Various Solvents and Their Composites

  • 투고 : 2017.09.29
  • 심사 : 2018.03.08
  • 발행 : 2018.03.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구에서는 5종의 약용식물(황금, 작약, 단삼, 상황버섯, 뽕잎)를 물, 50% 및 100% 주정으로 추출하여 항산화 활성, ACE 저해활성 및 콜레스테롤 흡착활성을 비교하였다. 그 중 상대적으로 활성이 우수한 작약, 상황버섯, 단삼을 0.2~2.0의 비율로 혼합한 조성물 7종의 활성을 검증함으로써 약용식물 조성물을 활용한 음료 개발시 기초 자료로 활용하고자 하였다. 약용식물 5종의 총 페놀화합물 함량은 물 추출물 중에서는 단삼 추출물이 80.27 mg/g으로 가장 높은 함량이었으며, 50% 및 100% 주정 추출물에서는 상황버섯이 각각 280.05 및 306.88 mg/g으로 가장 높았다. 플라보노이드 화합물도 단삼 추출물이 35.83 mg/g으로 물 추출물 중 가장 높았으며, 50% 및 100% 주정 추출물은 황금 추출물이 각각 62.71 및 64.59 mg/g으로 가장 높게 정량되었다. ACE 저해활성은 물 추출물에서만 활성이 있었는데, 황금 추출물에서 45.33%로 가장 활성이 높았다. 콜레스테롤 흡착능은 작약 물 추출물과 황금 100% 주정 추출물이 타 시료에 비해 유의적으로 활성이 높았다. DPPH와 ABTS 라디칼 소거활성 및 FRAP 활성을 통해 항산화 활성을 측정한 결과 물 추출물 중에서는 단삼 추출물의 활성이 가장 높았고, 50% 및 100% 주정 추출물의 경우 DPPH 라디칼 소거활성 및 FRAP는 상황버섯 추출물의 활성이 가장 높았고, ABTS 라디칼 소거활성은 작약 추출물에서 활성이 가장 높았다. 상황버섯, 작약 및 단삼 물 추출물의 혼합 비율을 달리하여 제조한 조성물 7종의 총 페놀 및 플라보노이드 화합물 함량은 각각 50.53~61.96 mg/g 및 16.91~33.84 mg/g의 범위였는데 단삼의 비율이 높은 시료에서 더 함량이 높았고, 콜레스테롤 흡착능은 46.27~70.03%로 작약 첨가 비율이 높은 시료에서 활성이 더 높았다. 항산화 활성도 단삼의 혼합 비율이 높을수록 더 높았는데, 항산화 활성과 콜레스테롤 흡착활성은 동일한 경향을 보이지 않아 조성물 제조시는 목표로 하는 활성에 따라서 조성물의 비율을 달리하는 것이 바람직할 것으로 판단된다.

This study was performed to investigate the antioxidative activity of solvent (water, 50% ethanol, and 100% ethanol) extracts from five kinds of medicinal herbs Cutellaria baicalensis Georgi; SB, Paeonia lactiflora Pall.; PA, Salvia miltiorrhiza Bunge; SM, Phellinus linteus; PH, Morus alba L.; MA). The total content of phenolic compounds was highest in the 50% ethanol extract from PH (280.05 mg/g), the 100% ethanol extract from PH (308.88 mg/g), and the water extract from SM (80.27 mg/g). The total content of flavonoids was highest in the 50% ethanol extract from SB (62.71 mg/ml), the 100% ethanol extract from SB (64.59 mg/ml), and the water extract from SM (35.85 mg/ml). ACE inhibitory activity only occurred in the water extracts, and it was highest in the water extract from SB (45.33%). Cholesterol adsorption activity was higher in the SB and PA extracts than in the other extracts. In water extracts, SM showed the highest antioxidative activity. Among the 50% and 100% ethanol extracts, DPPH radical scavenging activity and FRAP were highest in the PH extract, and ABTS radical scavenging activity was significantly higher in the PA extracts. Seven types of compositions were prepared with different mixing ratios of 0.2 to 2.0 from relatively high-activity medicinal herbs, such as PH, SM and PA. The total phenolic and flavonoid compound contents of the compositions were 50.53-61.96 and 16.91-33.81 mg/ml, respectively. Cholesterol adsorption activity was 46.27-70.03%.

키워드

참고문헌

  1. Ahn, I. S., Park, K. Y. and Do, M. S. 2007. Weight control mechanisms and antiobesity fundional agents. J. Kor. Soc. Food Sci. Nutr. 36, 503-513. https://doi.org/10.3746/jkfn.2007.36.4.503
  2. Bae, H. K., Hwang, I. W., Hong, H. D. and Shin, K. C. 2015. Antioxidant capacities and ${\beta}$-glucan content of ethanol extract from Phellinus baumii. Kor. J. Food Preserv. 22, 721-726.
  3. Benzie, I. F. F. and Strain, J. J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power". The FRAP assay. Anal. Biochem. 239, 70-76. https://doi.org/10.1006/abio.1996.0292
  4. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 181, 1199-1200. https://doi.org/10.1038/1811199a0
  5. Choi, K. S., Kim, Y. H. and Shin, K. O. 2016. Effect of mulberry extract on the lipid profile and liver function in mice fed a high fat diet. Kor. J. Food Nutr. 29, 411-419. https://doi.org/10.9799/ksfan.2016.29.3.411
  6. Choi, M. H., Kang, J. R., Sim, H. J., Kang, M. J., Seo, W. T., Bea, W. Y. and Shin, J. H. 2015. Physicochemical characteristics and antioxidant activity of Sumaeyaksuk depending on harvest times and processing methods. Kor. J. Food Preserv. 22, 399-407. https://doi.org/10.11002/kjfp.2015.22.3.399
  7. Choi, M. R., Lee, J. S. and Lim, H. S. 2007. Change in physiological activities of Scutellariae baicalensis by heating. J. Life Sci. 17, 1381-1386. https://doi.org/10.5352/JLS.2007.17.10.1381
  8. Choi, I. S., Cha, E. J., Lee, Y. R. and Kim, J. K. 2012. Antioxidant and anticancer activities of yak-sun tea prepared by oriental medicinal herbs. Kor. J. Food Nutr. 25, 447-453. https://doi.org/10.9799/ksfan.2012.25.3.447
  9. Cushman, D. W. and Cheung, I. S. 1917. Spectrophotometric assay and properties of the angiotensing-converting enzyme of rabbit lung. Biochem. Pharmacol. 20, 1637-1648.
  10. Do, J. R., Kim, K. J., Jo, J. H., Kim, Y. M., Kim, B. S., Kim, H. K., Lim, S. D. and Lee, S. W. 2005. Antimicrobial, antihypertensive and anticancer activities of medicinal herbs. Kor. J. Food Sci. Technol. 37, 206-213.
  11. Gutfinger, T. 1958. Polyphenols in oilve oils. JAOCS. 58, 966-968.
  12. Heo, H. J., Kim, Y. J., Chung, D. and Kim, D. O. 2007. Antioxidant capacities of individual and combined phenolics in a model system. Food Chem. 104, 87-92. https://doi.org/10.1016/j.foodchem.2006.11.002
  13. Jo, E. K., Gal, S. W. and Choi, Y. J. 2010. Antioxidative activity and angiotensin converting enzyme inhibitory activity of fermented medical plants (DeulBit) and its modulatory effects of nitric oxide production. J. Appl. Biol. Chem. 53, 91-98. https://doi.org/10.3839/jabc.2010.017
  14. Ju, J. C., Shin, J. H., Lee, S. J., Cho, H. S. and Sung, N. J. 2006. Antioxidative activity of hot water extracts from medicinal plants. J. Kor. Soc. Food Sci. Nutr. 35, 7-14. https://doi.org/10.3746/jkfn.2006.35.1.007
  15. Joo, S. Y. 2013. Antioxidant activities of medicinal plant extracts. J. Kor. Soc Food Sci. Nutr. 42, 512-519. https://doi.org/10.3746/jkfn.2013.42.4.512
  16. Jung, M. H., Lee, S. S., Park, S. H. and Hwang, H. J. 2013. The antioxidative effect of ethanol extracts from Lithospermum erythrorhizon Siebold & Zucc., Xanthium strumarium Linn, and Lonicera japonica. J. Life Sci. 23, 643-649. https://doi.org/10.5352/JLS.2013.23.5.643
  17. Kang, J. R., Kang, M. J., Choi, M. H., Byun, H. U. and Shin, J. H. 2017. Physicochemical characteristics of ethanol extract form Artemisia Argyi H. Using different preparation methods. J. Life Sci. 27, 23-31. https://doi.org/10.5352/JLS.2017.27.1.23
  18. Kang, M. J., Hwang, C. R., Lee, S. J. and Shin, J. H. 2015. Antioxidant and anti-inflammatory activity of medicinal herbs composites. J. Agric. Life Sci. 49, 279-292.
  19. Kang, Y. M., Kim, S. H., Lee, Y. C., Kim, H. K. and Kim, D. S. 2014. Synergistic combination effect of anti-obesity in the extracts of Phyllostachys pubescence Mael and Scutellaria baicalensis Georgi. Kor. J. Herbology 29, 7-13.
  20. Kim, E. J., Choi, J. Y., Yu, M. R., Kim, M. Y., Lee, S. H. and Lee, B. H. 2012. Total polyphenols, total flavonoid contents, and antioxidant activity of Korean natural and medicinal plants. Kor. J. Food Sci. Technol. 44, 337-342. https://doi.org/10.9721/KJFST.2012.44.3.337
  21. Kim, S. J., Park, J. H., Choi, S. Y. and Kim, K. Y. 2006. Change of phenolic compounds affected by different drying method in leaves and stems of peony (Paeonia lactiflora Pall.). Kor. J. Crop Sci. 51, 251-254.
  22. Kim, M. H., Kim, M. C., Park, J. S., Kim, J. W. and Lee, J. O. 2001. The antioxidative effects of the water-soluble extracts of plants used as tea materials. Kor. J. Food Sci. Technol. 33, 12-18.
  23. Ko, J. B. 2006. Effects of Cheonggukjang added Phellinus linteus on lipid metabolism in hyperlipidemic rats. J. Kor. Soc. Food Sci. Nutr. 35, 410-415. https://doi.org/10.3746/jkfn.2006.35.4.410
  24. Kwon, E. K., Kim, Y. E., Lee, C. H. and Kim, H. Y. 2006. Screening of nine herbs with biological activities on ACE inhibiton, HMG-CoA reductase inhibition, and fibrinolysis. Kor. J. Food Sci. Technol. 38, 691-698.
  25. Kwon, Y. S., Lee, H. G., Shin, H. K. and Yang, C. B. 2000. Purification and identification of angiotensin I converting enzyme inhibitory peptide from small red bean protein hydrolyzate. Food Sci. Biotechol. 9, 292-296.
  26. Lee, G. M. and Lee, K. S. 2004. Effects of CPs (combined preparations) of Radix Salvia Miltiorrhiza, Radix Notoginseng, and Borneolum on hyperlipidemia. J. Kor. Oriental Med. 25, 22-32.
  27. Lee, J. M., Choi, S. W., Cho, S. H. and Rhee, S. J. 2003. Effect of seeds extract of Paeonia Lactiflara on antioxidative system and lipid peroxidation of liver in rats fed high-cholesterol diet. Kor. J. Nutr. 36, 793-800.
  28. Lee, M. J., Oh, J. S., Park, J. W., Kim, J, K., Choi, D. Y. and Kim, C. H. 2000. Antioxidant activity of extract from Scutellaria baicalensis Georgi. J. Life Sci. 10, 236-240.
  29. Lee, S. E., Lee, J. H., Kim, J. K., Kim, G. S., Kim, Y. O., Soe, J. S., Choi, J. H., Lee, E. S., Noh, H. J. and Kim, S. Y. 2011. Anti-inflammatory activity of medicinal plant extracts. Kor. J. Med. Crop Sci. 19, 217-226. https://doi.org/10.7783/KJMCS.2011.19.4.217
  30. Lee, S. E., Seong, N. S., Bang, J. K., Park, C. G., Sung, J. S. and Song, J. 2003. Antioxidative activities of Korean medicinal plants. Kor. J. Medicinal Crop Sci. 11, 127-134.
  31. Lee, S. G., Jeong, H. J., Lee, E. J., Kim, J. B. and Choi, S. W. 2011. Antioxidant and anti-inflammatory activities of ethanol extracts from medicinal herb mixtures. Kor. J. Food Sci. Technol. 43, 200-205. https://doi.org/10.9721/KJFST.2011.43.2.200
  32. Lee, S. J., Shin, J. H., Lee, H. J., Tak, H. M., Kang, M. J. and Sung, N. J. 2013. Antioxidant and anti-inflammatory activities of functional plant Materials. J. Life Sci. 23, 869-878. https://doi.org/10.5352/JLS.2013.23.7.869
  33. Moreno, M. I. N., Isla, M. I., Sampietro, A. R. and Vattuone, M. A. 2000. Comparison of the free radical scavenging activity of propolis from several region of Argentina. J. Ethnopharmacol. 71, 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  34. Park, J. G., Lee, J. M. and Jun, W. J. 2013. Radical scavenging and anti-obesity effects of various extracts from turmeric (Curcuma longa L.). J. Kor. Soc. Food Sci. Nutr. 42, 1908-1914. https://doi.org/10.3746/jkfn.2013.42.12.1908
  35. Park, Y. S. 2002. Antioxidative activities and contents of polyphenolic compound of medicinal herb extracts. J. East Asian Soc. Dietary Life. 12, 23-31.
  36. Seo, S. H., Lee, H. R., Rhee, S. J., Choi, S. W. and Cho, S. H. 2003. Effects of peonia seed extracts and resveratrol on lipid metabolism in rats fed high cholesterol diets. J. Kor. Soc. Food Sci. Nutr. 32, 1102-1107. https://doi.org/10.3746/jkfn.2003.32.7.1102
  37. Soh, H. S., Kim, C. S. and Lee, S. P. 2003. A new in vitro assay of cholesterol adsorption by food and microbial polysaccharides. J. Med. Food 6, 225-230. https://doi.org/10.1089/10966200360716643
  38. Song, W. Y. and Choi, J. H. 2017. Total phenols, flavonoid contents, and antioxidant activity of Spirodela polyrhiza extracts. J. Life Sci. 27, 180-186. https://doi.org/10.5352/JLS.2017.27.2.180
  39. Song, W. Y., Kim, Y. N., Chun, S. S., Ku, K. H. and Choi, J. H. 2011. Effects of ethanol extracts from red pepper (Capsicum annuum L.) seeds on cholesterol adsorption capacity and UDP-glucuronyl transferase activity. J. Life Sci. 21, 829-837. https://doi.org/10.5352/JLS.2011.21.6.829
  40. Yang, S. A., Im, N. K. and Lee, I. S. 2007. Effects of methanolic extract from Salvia miltiorrhiza Bunge on in vitro antithrombotic and antioxidative activities. Kor. J. Food Sci. Technol. 39, 83-87.
  41. Yoon, H. J. and Park, Y. S. 2010. Effects of Scutellaria baicalensis water extract on lipid metabolism and antioxidant defense system in rats fed high fat diet. J. Kor. Soc. Food Sci. Nutr. 39, 219-226. https://doi.org/10.3746/jkfn.2010.39.2.219